Effect of pectin on epsilon-polylysine purification: Study on preparation, physicochemical property, and bioactivity of pectin-epsilon-polylysine complex

• Published in: Food hydrocolloids Vol. 124; p. 107314
• Main Authors: Chang, Sen-Lin, Zhao, Qing-Sheng, Li, Hang, Wang, Xiao-Dong, Wang, Li-Wei, Zhao, Bing
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.03.2022
• Subjects: Antibacterial performance; Epsilon-polylysine; Pectin; Purification; Pectin; Epsilon-polylysine; Purification; Antibacterial performance
• ISSN: 0268-005X; 1873-7137
• DOI: 10.1016/j.foodhyd.2021.107314

Epsilon-polylysine (ε-PL) is a typical antibacterial peptide with a wide spectrum and high safety. Pectin could form electrostatic complex with ε-PL and affects the antibacterial performance. However, the potential application of pectin on ε-PL purification has not been discussed. In this study, a novel process for preparation antibacterial complex pectin-ε-PL was carried out by adding pectin to the ε-PL fermentation broth and further processing. The results showed that ε-PL could be completely collected when the mass ratio of pectin to ε-PL was 8. After further removing pectin by ethanol precipitation, pectin-ε-PL complex was obtained. The content of ε-PL in pectin-ε-PL complex was 30.59% ± 1.21%, and the recovery rate of ε-PL was 62.63% ± 0.63%. In addition, the physicochemical properties of pectin-ε-PL complex were discussed. The Zeta-potential analysis suggested that the intrinsic pK of pectin-ε-PL complex was 7.74. Furthermore, the cytotoxicity test showed that for Raw264.7 cells pectin-ε-PL was a more compatible and safer product than ε-PL alone. At last, the minimal inhibitory concentration (MIC) of pectin-ε-PL for B. subtilis, and E. coli were 0.25 mg/mL and 0.1 mg/mL respectively, which presented the same activity as high purity ε-PL. Therefore, the separation of ε-PL from fermentation broth by pectin is a novel promising technological process, and the obtained pectin-ε-PL complex is an outstanding product with high stability and antibacterial performance. [Display omitted] •The epsilon-polylysine was obtained from fermentation broth in the form of pectin-epsilon-polylysine complex by a new procedure using pectin.•The formation of Pectin-epsilon-polylysine complex could strengthen the stability of epsilon-polylysine in solution.•Pectin-epsilon-polylysine complex have better antibacterial performance than polylysine itself.