Optimization of Melanin Extraction from the Wood Ear Medicinal Mushroom, Auricularia auricula-judae (Agaricomycetes), by Response Surface Methodology and Its Antioxidant Activities In Vitro

• Published in: International journal of medicinal mushrooms Vol. 20; no. 11; p. 1087
• Main Authors: Ma, Yin-Peng, Bao, Yi-Hong, Kong, Xiang-Hui, Tian, Jiao-Jiao, Han, Bing, Zhang, Jie-Chi, Chen, Xi-Jun, Zhang, Pi-Qi, Wang, Hui, Dai, Xiao-Dong, Liu, Jia-Ning, Han, Zeng-Hua, Ma, Qing-Fang
• Format: Journal Article
• Language: English
• Published: United States 2018
• ISSN: 1940-4344
• DOI: 10.1615/IntJMedMushrooms.2018028694

The optimal conditions for melanin extraction from Auricularia auricula-judae (Hei 29) fruiting bodies was determined on the basis of the extract yield of melanin, calculated by using a single-factor experiment and response surface methodology. Its antioxidant activities were also studied in vitro. Various optimal process conditions for melanin extraction were determined by using Design-Expert software: incubation temperature, 69.11°C; incubation time, 58.66 minutes; and incubation pH, 12.81. Under these conditions, the melanin yield was 2.59%. We found that the antioxidant activities of A. auricula-judae melanin in vitro were strong against DPPH radicals and superoxide anions. The rate of DPPH radical scavenging was 63.04% when the A. auricula-judae melanin concentration was 0.36 mg/mL; the rate of superoxide anion scavenging reached 39.79% when the concentration was 0.375 mg/mL. However, the antioxidant activity against hydroxyl radicals was somewhat weak; the rate of scavenging reached only 7.47% when the A. auricula-judae melanin concentration was 0.06 mg/mL.