Quantitative determination of 1,2‐diacylglycerol in thoracic aorta of the rat using latroscan TLC/FID: Effect of norepinephrine

• Published in: Lipids Vol. 24; no. 11; pp. 982 - 984
• Main Authors: Okumura, Kenji, Yamada, Yumiko, Kondo, Junichiro, Kobayashi, Norio, Hashimoto, Hidekazu, Ito, Takayuki
• Format: Journal Article
• Language: English; Japanese
• Published: Berlin/Heidelberg Wiley 01.11.1989; Springer‐Verlag; Springer
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Aorta, Thoracic; Biological and medical sciences; Cholesterol; Chromatography, Gas; Chromatography, Thin Layer; Diglycerides; Flame Ionization; Fundamental and applied biological sciences. Psychology; Glycerides; Lipids; Male; Norepinephrine; Other biological molecules; Rats; Rats, Inbred Strains; Elution; Separation; Rat; Rodentia; Aminoacid derivative hormone; Lipids; Flame ionization detector; Catecholamine; Thin layer chromatography; Vertebrata; Mammalia; Neurotransmitter; Aorta; Norepinephrine; Quantitative analysis

This study was undertaken to evaluate the use of the Iatroscan TLC/FID system for quantitating 1,2‐diacylglycerol (DG) in the aorta. Cholesteryl acetate was chosen as an internal standard. In order to avoid interference of triglyceride and phospholipids with the separation of the internal standard a...