Quantitative determination of 1,2‐diacylglycerol in thoracic aorta of the rat using latroscan TLC/FID: Effect of norepinephrine
This study was undertaken to evaluate the use of the Iatroscan TLC/FID system for quantitating 1,2‐diacylglycerol (DG) in the aorta. Cholesteryl acetate was chosen as an internal standard. In order to avoid interference of triglyceride and phospholipids with the separation of the internal standard a...
Saved in:
| Published in | Lipids Vol. 24; no. 11; pp. 982 - 984 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English Japanese |
| Published |
Berlin/Heidelberg
Wiley
01.11.1989
Springer‐Verlag Springer |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | This study was undertaken to evaluate the use of the Iatroscan TLC/FID system for quantitating 1,2‐diacylglycerol (DG) in the aorta. Cholesteryl acetate was chosen as an internal standard. In order to avoid interference of triglyceride and phospholipids with the separation of the internal standard and 1,2‐DG, a stepwise elution of lipids from the silicic acid column was used. The development of Chromarods was done using two solvent systems and a three‐step developing technique. Assay and recovery of both 1,2‐DG and cholesterol (as compared to cholesteryl acetate) were sufficient to measure changes in the 1,2‐DG content in blood vessels. After exposing the thoracic aorta to 10−5 M norepinephrine for 10 min, the 1,2‐DG content increased nearly two‐fold without significant change in cholesterol content. |
|---|---|
| AbstractList | This study was undertaken to evaluate the use of the Iatroscan TLC/FID system for quantitating 1,2‐diacylglycerol (DG) in the aorta. Cholesteryl acetate was chosen as an internal standard. In order to avoid interference of triglyceride and phospholipids with the separation of the internal standard and 1,2‐DG, a stepwise elution of lipids from the silicic acid column was used. The development of Chromarods was done using two solvent systems and a three‐step developing technique. Assay and recovery of both 1,2‐DG and cholesterol (as compared to cholesteryl acetate) were sufficient to measure changes in the 1,2‐DG content in blood vessels. After exposing the thoracic aorta to 10−5 M norepinephrine for 10 min, the 1,2‐DG content increased nearly two‐fold without significant change in cholesterol content. This study was undertaken to evaluate the use of the Iatroscan TLC/FID system for quantitating 1,2‐diacylglycerol (DG) in the aorta. Cholesteryl acetate was chosen as an internal standard. In order to avoid interference of triglyceride and phospholipids with the separation of the internal standard and 1,2‐DG, a stepwise elution of lipids from the silicic acid column was used. The development of Chromarods was done using two solvent systems and a three‐step developing technique. Assay and recovery of both 1,2‐DG and cholesterol (as compared to cholesteryl acetate) were sufficient to measure changes in the 1,2‐DG content in blood vessels. After exposing the thoracic aorta to 10 −5 M norepinephrine for 10 min, the 1,2‐DG content increased nearly two‐fold without significant change in cholesterol content. |
| Author | Norio Kobayashi Junichiro Kondo Takayuki Ito Hidekazu Hashimoto Kenji Okumura Yumiko Yamada |
| Author_xml | – sequence: 1 givenname: Kenji surname: Okumura fullname: Okumura, Kenji organization: Nagoya University School of Medicine – sequence: 2 givenname: Yumiko surname: Yamada fullname: Yamada, Yumiko organization: Nagoya University School of Medicine – sequence: 3 givenname: Junichiro surname: Kondo fullname: Kondo, Junichiro organization: Nagoya University School of Medicine – sequence: 4 givenname: Norio surname: Kobayashi fullname: Kobayashi, Norio organization: Nagoya University School of Medicine – sequence: 5 givenname: Hidekazu surname: Hashimoto fullname: Hashimoto, Hidekazu organization: Nagoya University School of Medicine – sequence: 6 givenname: Takayuki surname: Ito fullname: Ito, Takayuki organization: Nagoya University School of Medicine |
| BackLink | https://cir.nii.ac.jp/crid/1870865117541365248$$DView record in CiNii http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6692265$$DView record in Pascal Francis |
| BookMark | eNp9kMFO3DAQhi1EJbaUS5_AB06oKWPHTmxudGHblVaCSvQcTRybtRTslWNa7Q3eoM_YJ6m3tCD1wGVGI30z8___W7IfYrCEvGfwkQG0p70DLoWQQu6RGZNSVbqGdp_MALioBAd2QI6myfcgldC1bGFGHr_eY8g-Y_bfLR1stunOhzLFQKOj7AP_9fBz8Gi24-24NTbFkfpA8zomNN5QjCnjjsxrSxNmej_5cEtHzClOBgO9Wc1PF8uLM3rpnDV5h4aY7MYHu1mnUt-RNw7HyR797Yfk2-LyZv6lWl19Xs7PV5WpQfNKF3NWWxRguOG2xr54ZUoq4M5p00s9cKMGBnUv7eBEYdqGSS2QyR4A6kNy_HR3g0XY6BIG46duk_wdpm3XNJrzRhbs5AkzxcCUrHsmGHS7mLtPi38xFxj-g82fKGPICf346soPP9rtK8e71fL6ArTiL8qD9-XBrjLVgmokY20JoW4kF6r-DVpSmrQ |
| CODEN | LPDSAP |
| CitedBy_id | crossref_primary_10_1016_j_chroma_2009_07_061 crossref_primary_10_1039_np9971400163 crossref_primary_10_1016_0003_2697_91_90537_4 |
| Cites_doi | 10.1007/BF02535467 10.1016/S0021-9258(18)49255-1 10.1016/0003-2697(74)90315-7 10.1073/pnas.83.18.6785 10.1073/pnas.76.7.3238 10.1016/S0021-9258(19)75758-5 10.1126/science.3107122 10.1126/science.6147898 10.1016/S0021-9258(18)33114-4 |
| ContentType | Journal Article |
| Copyright | 1989 American Oil Chemists' Society (AOCS) 1990 INIST-CNRS |
| Copyright_xml | – notice: 1989 American Oil Chemists' Society (AOCS) – notice: 1990 INIST-CNRS |
| DBID | RYH AAYXX CITATION IQODW |
| DOI | 10.1007/bf02544545 |
| DatabaseName | CiNii Complete CrossRef Pascal-Francis |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | CrossRef |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology |
| EISSN | 1558-9307 |
| EndPage | 984 |
| ExternalDocumentID | 6692265 10_1007_BF02544545 LIPD0982 |
| Genre | article |
| GroupedDBID | --- -~C -~X .55 .86 .GJ .VR 0R~ 1N0 1OB 1OC 1SB 2.D 203 28- 29L 2JY 2VQ 4.4 40D 53G 5GY 5RE 6NX 875 95. 95~ AAHBH AAIAL AANXM AAYZH ABJNI ABMNI ABQSL ACBXY ACCZN ACGFS ACHXU ACIWK ACPRK ACXQS ADHKG ADKYN ADZMN AEJHL AEKMD AENEX AEUYR AEYWJ AFBBN AFEXP AFLOW AFRAH AGGDS AGQPQ AHKAY AHMBA AKMHD ALIPV ALMA_UNASSIGNED_HOLDINGS AMKLP ASPBG AVWKF AZFZN B-. BBWZM BLYAC CS3 DU5 EBS EJD EMOBN F5P FEDTE H13 HVGLF HZ~ KOV LATKE M4Y N2Q N9A NDZJH NF0 NU0 O9- O93 O9G O9I P19 P2P PT5 R4E R89 R9I RGG RNI ROL RSV RYH RZK S26 S27 S28 SAMSI SAP SDM SOJ T13 T16 W4F WH7 WXSBR X7M ZCG ZGI ~02 ~EX ~KM -4W -58 -5G -BR -Y2 06C 06D 0VY 199 29~ 2J2 2KG 2KM 2LR 2~H 30V 33P 3O- 3V. 408 40E 41~ 5VS 67Z 78A 7X7 88A 88E 8AO 8C1 8CJ 8FE 8FH 8FI 8FJ 8TC 8UJ 95- 96X AAAVM AABHQ AAHHS AAHQN AAJKR AAMNL AANLZ AARHV AARTL AASGY AAWCG AAXRX AAYCA AAYIU AAYQN AAYTO AAZKR ABCUV ABHLI ABJOX ABNWP ABPLI ABTEG ABTHY ABTMW ABUWG ACAHQ ACCFJ ACKNC ACOMO ACPOU ACXBN ADBBV ADHIR ADINQ ADKPE ADOZA ADRFC ADXAS AEBTG AEEZP AEGAL AEGNC AEIGN AEOHA AEPYU AEQDE AETLH AEUYN AFFPM AFGCZ AFKRA AFWTZ AFWVQ AFZKB AGAYW AGJBK AGQMX AGWIL AGWZB AGYKE AHAVH AHBTC AHBYD AHSBF AHYZX AIIXL AITYG AIURR AIWBW AJBDE AJBLW AJRNO ALUQN ALVPJ ALWAN AMYDB ARMRJ BA0 BBNVY BENPR BFHJK BGNMA BHPHI BKSAR BPHCQ BVXVI CAG CCPQU COF CSCUP D1J DCZOG DRFUL DRSTM ESBYG FIGPU FNLPD FRRFC FWDCC FYUFA G-Y G-Z GGCAI GGRSB GQ6 GQ7 HCIFZ HF~ HG5 HG6 HGLYW HMCUK HMJXF HRMNR IJ- ITM IXC IXE IZQ I~X I~Z J-C J0Z JBSCW KDC LEEKS LK8 LUTES LYRES M0L M1P M7P MA- MEWTI NB0 O9J OAM P0- P2W P9N PCBAR PF0 PQQKQ PROAC PSQYO Q2X QOR QOS RIG RPX S16 S1Z S3B SCM SDH SHX SJN SNE SNX SUPJJ SZN TSG TSK TSV TUC U2A U9L UG4 UKHRP VC2 W48 WK8 WOHZO WYJ Y6R Z45 Z7U Z7V Z7W Z7Y Z87 Z8O Z8P Z8Q Z8S Z91 ZOVNA ZZTAW AAYXX ABFSG ACSTC AEZWR AFHIU AGHNM AGYGG AHWEU AIXLP CITATION PHGZM PHGZT AAMMB AEFGJ AGXDD AIDQK AIDYY IQODW PJZUB PPXIY PQGLB |
| ID | FETCH-LOGICAL-c3092-9f02e9ea40c2c2e3ab254185802ff9cb59d2c8d103b5edf4c2e761594a15b0003 |
| ISSN | 0024-4201 |
| IngestDate | Mon Jul 21 09:16:32 EDT 2025 Tue Jul 01 01:07:58 EDT 2025 Thu Apr 24 23:11:52 EDT 2025 Wed Jan 22 16:22:53 EST 2025 Thu Jun 26 22:29:53 EDT 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 11 |
| Keywords | Elution Separation Rat Rodentia Aminoacid derivative hormone Lipids Flame ionization detector Catecholamine Thin layer chromatography Vertebrata Mammalia Neurotransmitter Aorta Norepinephrine Quantitative analysis |
| Language | English Japanese |
| License | http://onlinelibrary.wiley.com/termsAndConditions#vor CC BY 4.0 |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c3092-9f02e9ea40c2c2e3ab254185802ff9cb59d2c8d103b5edf4c2e761594a15b0003 |
| PageCount | 3 |
| ParticipantIDs | pascalfrancis_primary_6692265 crossref_primary_10_1007_BF02544545 crossref_citationtrail_10_1007_BF02544545 wiley_primary_10_1007_BF02544545_LIPD0982 nii_cinii_1870865117541365248 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 1900 |
| PublicationDate | November 1989 |
| PublicationDateYYYYMMDD | 1989-11-01 |
| PublicationDate_xml | – month: 11 year: 1989 text: November 1989 |
| PublicationDecade | 1980 |
| PublicationPlace | Berlin/Heidelberg |
| PublicationPlace_xml | – name: Berlin/Heidelberg – name: Heidelberg |
| PublicationTitle | Lipids |
| PublicationYear | 1989 |
| Publisher | Wiley Springer‐Verlag Springer |
| Publisher_xml | – name: Wiley – name: Springer‐Verlag – name: Springer |
| References | 1987; 262 1987; 240 1974; 59 1988; 23 1982; 258 1986; 83 1979; 76 1987; 236 1984; 225 e_1_2_2_4_1 e_1_2_2_5_1 e_1_2_2_6_1 e_1_2_2_7_1 e_1_2_2_11_1 e_1_2_2_10_1 Chiu A.T. (e_1_2_2_8_1) 1987; 240 e_1_2_2_2_1 e_1_2_2_3_1 e_1_2_2_9_1 |
| References_xml | – volume: 59 start-page: 617 year: 1974 end-page: 627 publication-title: Anal. Biochem. – volume: 83 start-page: 6785 year: 1986 end-page: 6789 publication-title: Proc. Natl. Acad. Sci. USA – volume: 258 start-page: 764 year: 1982 end-page: 769 publication-title: J. Biol. Chem. – volume: 225 start-page: 1365 year: 1984 end-page: 1370 publication-title: Science – volume: 236 start-page: 586 year: 1987 end-page: 589 publication-title: Science – volume: 240 start-page: 123 year: 1987 end-page: 127 publication-title: J. Pharmacol Exp Ther. – volume: 23 start-page: 253 year: 1988 end-page: 255 publication-title: Lipids – volume: 262 start-page: 16305 year: 1987 end-page: 16313 publication-title: J. Biol. Chem. – volume: 76 start-page: 3238 year: 1979 end-page: 3241 publication-title: Proc. Natl. Acad. Sci. USA – volume: 262 start-page: 1116 year: 1987 end-page: 1121 publication-title: J. Biol. Chem. – ident: e_1_2_2_7_1 doi: 10.1007/BF02535467 – ident: e_1_2_2_3_1 doi: 10.1016/S0021-9258(18)49255-1 – ident: e_1_2_2_6_1 doi: 10.1016/0003-2697(74)90315-7 – ident: e_1_2_2_9_1 doi: 10.1073/pnas.83.18.6785 – ident: e_1_2_2_4_1 doi: 10.1073/pnas.76.7.3238 – ident: e_1_2_2_11_1 doi: 10.1016/S0021-9258(19)75758-5 – ident: e_1_2_2_10_1 doi: 10.1126/science.3107122 – volume: 240 start-page: 123 year: 1987 ident: e_1_2_2_8_1 publication-title: J. Pharmacol Exp Ther. – ident: e_1_2_2_2_1 doi: 10.1126/science.6147898 – ident: e_1_2_2_5_1 doi: 10.1016/S0021-9258(18)33114-4 |
| SSID | ssib058493570 ssj0021495 ssib054405773 ssib010622863 |
| Score | 1.3680867 |
| Snippet | This study was undertaken to evaluate the use of the Iatroscan TLC/FID system for quantitating 1,2‐diacylglycerol (DG) in the aorta. Cholesteryl acetate was... |
| SourceID | pascalfrancis crossref wiley nii |
| SourceType | Index Database Enrichment Source Publisher |
| StartPage | 982 |
| SubjectTerms | Analytical, structural and metabolic biochemistry Animals Aorta, Thoracic Biological and medical sciences Cholesterol Chromatography, Gas Chromatography, Thin Layer Diglycerides Flame Ionization Fundamental and applied biological sciences. Psychology Glycerides Lipids Male Norepinephrine Other biological molecules Rats Rats, Inbred Strains |
| Title | Quantitative determination of 1,2‐diacylglycerol in thoracic aorta of the rat using latroscan TLC/FID: Effect of norepinephrine |
| URI | https://cir.nii.ac.jp/crid/1870865117541365248 https://onlinelibrary.wiley.com/doi/abs/10.1007%2FBF02544545 |
| Volume | 24 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3LbtNAFB3RdsMGUQqihaKRQEhVMNjjsePpLkmJ0lIKSKnELrLHY3CbxlGIF-kK_oBv5Es4M37EFhWvjRU51-PI9-Q-xufeS8gzV8RdeJrQikQQW9xNPEv4ibJCOFuPR8pXji4Ufnvmj875yUev8aLdVJcso5fy-sa6kv_RKs5Br7pK9h80Wy-KE_gM_eIIDeP4Vzr-kIczUySm6T9xRWypYkDYqAGryQzAgVxNP01XUmlquqE3Qv1S92vVIXhFFgAiOrnZQDC1Ql-0lRyfDvAjh8dHev-g7HesCSDZQs0RpQIP1dv5qrI6nadxHa2P0lhdhtd5Z6QnNwEbWcPMX6Sdd5f5Vb6o_cOJLlj5nC6yzhs9a6Ters4WqT4VhSu9zHq7wvCxnNZ2hbF1TYPMuMVZKaBKG-zBBrvFMNzKSDPesLKimFdUOmxRzJj7xRcU9I8oKbqwcW-DbDHkEjCGW71hv39WmR0kxYwF6y5kkEYQu47qEKIJ18z6K_N4nVQWzKHitze63q7v1opzNmZpqlm3IZQ2TYqJKe1syIQz47vkTpmH0F4Bqm1y6yK8R3Z6QE92taLPqWEGm1cuO-RbE2e0hTOaJdR5wX58_d7GF01ntMIXNfjSksAXBb6owRet8UWBr1dA1yEtsKVF29i6T86Hr8eDkVWO7rCkawtmCTwHJVTIbckkU24Y4bEgNAxsliRCRp6ImQxix3YjT8UJh0wXsbXgoeOZPP0B2ZxlM_WQUI4ctislXA9XPAnsUF-Ai4VUIuJde5ccVA96Isu-9nq8ynRSdeTuDyul7JKntey86OZyo9Q-9IXF9NGBQwt8T3e05ZoSyniA71uarJfyfYFsBtcfGM3-5haT0-P3RzZwvPeHez0it9f_osdkc7nI1T7C4GX0pITxT5SlqtU |
| linkProvider | Library Specific Holdings |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Quantitative+determination+of+1%2C2%E2%80%90diacylglycerol+in+thoracic+aorta+of+the+rat+using+latroscan+TLC%2FFID%3A+Effect+of+norepinephrine&rft.jtitle=Lipids&rft.au=Hidekazu+Hashimoto&rft.au=Kenji+Okumura&rft.au=Junichiro+Kondo&rft.au=Norio+Kobayashi&rft.date=1989-11-01&rft.pub=Wiley&rft.issn=0024-4201&rft.eissn=1558-9307&rft.volume=24&rft.spage=982&rft.epage=984&rft_id=info:doi/10.1007%2Fbf02544545 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0024-4201&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0024-4201&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0024-4201&client=summon |