COTIP: Cotton TILLING Platform, a Resource for Plant Improvement and Reverse Genetic Studies
Cotton is cultivated worldwide for its white fiber, of which around 90% is tetraploid upland cotton ( L.) carrying both A and D genome. Since centuries, yield increasing efforts for the cotton crop by conventional breeding approaches have caused an extensive erosion of natural genetic variability. M...
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Published in | Frontiers in plant science Vol. 7; p. 1863 |
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Abstract | Cotton is cultivated worldwide for its white fiber, of which around 90% is tetraploid upland cotton (
L.) carrying both A and D genome. Since centuries, yield increasing efforts for the cotton crop by conventional breeding approaches have caused an extensive erosion of natural genetic variability. Mutation based improvement strategies provide an effective way of creating new allelic variations. Targeting Induced Local Lesions IN Genomes (TILLING) provides a mutation based reverse genetic strategy to create and evaluate induced genetic variability at DNA level. Here, we report development and testing of TILLING populations of allotetraploid cotton (
) for functional genomic studies and mutation based enrichment of cotton genetic resources. Seed of two cotton cultivars "PB-899 and PB-900" were mutagenized with 0.3 and 0.2% (v/v) ethyl methanesulfonate, respectively. The phenotyping of M
and M
populations presented numerous mutants regarding the branching pattern, leaf morphology, disease resistance, photosynthetic lesions and flower sterility. Molecular screening for point mutations was performed by TILLING PCR aided CEL1 mismatch cleavage. To estimate the mutation frequency in the mutant genomes, five gene classes were TILLed in 8000 M
plants of each var. "PB-899" and "PB-900." These include actin (
), Pectin Methyl Esterase (
), sucrose synthase (
), resistance gene analog, and defense response gene (
). The var. PB-899 was harboring 47% higher mutation induction rate than PB-900. The highest rate of mutation frequency was identified for NAC-TF5 (EU706348) of DRGs class, ranging from 1/58 kb in PB-899 to 1/105 kb in PB-900. The mutation screening assay revealed the presence of significant proportion of induced mutations in cotton TILLING populations such as 1/153 kb and 1/326 kb in var. "PB-899" and "PB-900," respectively. The establishment of a cotton TILLING platform (COTIP) and data obtained from the resource TILLING population suggest its effectiveness in widening the genetic bases of cotton for improvement and utilizing it for subsequent reverse genetic studies of various genes. |
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AbstractList | Cotton is cultivated worldwide for its white fiber, of which around 90% is tetraploid upland cotton (
L.) carrying both A and D genome. Since centuries, yield increasing efforts for the cotton crop by conventional breeding approaches have caused an extensive erosion of natural genetic variability. Mutation based improvement strategies provide an effective way of creating new allelic variations. Targeting Induced Local Lesions IN Genomes (TILLING) provides a mutation based reverse genetic strategy to create and evaluate induced genetic variability at DNA level. Here, we report development and testing of TILLING populations of allotetraploid cotton (
) for functional genomic studies and mutation based enrichment of cotton genetic resources. Seed of two cotton cultivars "PB-899 and PB-900" were mutagenized with 0.3 and 0.2% (v/v) ethyl methanesulfonate, respectively. The phenotyping of M
and M
populations presented numerous mutants regarding the branching pattern, leaf morphology, disease resistance, photosynthetic lesions and flower sterility. Molecular screening for point mutations was performed by TILLING PCR aided CEL1 mismatch cleavage. To estimate the mutation frequency in the mutant genomes, five gene classes were TILLed in 8000 M
plants of each var. "PB-899" and "PB-900." These include actin (
), Pectin Methyl Esterase (
), sucrose synthase (
), resistance gene analog, and defense response gene (
). The var. PB-899 was harboring 47% higher mutation induction rate than PB-900. The highest rate of mutation frequency was identified for NAC-TF5 (EU706348) of DRGs class, ranging from 1/58 kb in PB-899 to 1/105 kb in PB-900. The mutation screening assay revealed the presence of significant proportion of induced mutations in cotton TILLING populations such as 1/153 kb and 1/326 kb in var. "PB-899" and "PB-900," respectively. The establishment of a cotton TILLING platform (COTIP) and data obtained from the resource TILLING population suggest its effectiveness in widening the genetic bases of cotton for improvement and utilizing it for subsequent reverse genetic studies of various genes. Cotton is cultivated worldwide for its white fiber, of which around 90% is tetraploid upland cotton ( Gossypium hirsutum L.) carrying both A and D genome. Since centuries, yield increasing efforts for the cotton crop by conventional breeding approaches have caused an extensive erosion of natural genetic variability. Mutation based improvement strategies provide an effective way of creating new allelic variations. Targeting Induced Local Lesions IN Genomes (TILLING) provides a mutation based reverse genetic strategy to create and evaluate induced genetic variability at DNA level. Here, we report development and testing of TILLING populations of allotetraploid cotton ( G. hirsutum ) for functional genomic studies and mutation based enrichment of cotton genetic resources. Seed of two cotton cultivars “PB-899 and PB-900” were mutagenized with 0.3 and 0.2% (v/v) ethyl methanesulfonate, respectively. The phenotyping of M 1 and M 2 populations presented numerous mutants regarding the branching pattern, leaf morphology, disease resistance, photosynthetic lesions and flower sterility. Molecular screening for point mutations was performed by TILLING PCR aided CEL1 mismatch cleavage. To estimate the mutation frequency in the mutant genomes, five gene classes were TILLed in 8000 M 2 plants of each var. “PB-899” and “PB-900.” These include actin ( GhACT ), Pectin Methyl Esterase ( GhPME ), sucrose synthase ( GhSUS ), resistance gene analog, and defense response gene ( DRGs ). The var. PB-899 was harboring 47% higher mutation induction rate than PB-900. The highest rate of mutation frequency was identified for NAC-TF5 (EU706348) of DRGs class, ranging from 1/58 kb in PB-899 to 1/105 kb in PB-900. The mutation screening assay revealed the presence of significant proportion of induced mutations in cotton TILLING populations such as 1/153 kb and 1/326 kb in var. “PB-899” and “PB-900,” respectively. The establishment of a cotton TILLING platform (COTIP) and data obtained from the resource TILLING population suggest its effectiveness in widening the genetic bases of cotton for improvement and utilizing it for subsequent reverse genetic studies of various genes. |
Author | Aslam, Usman Cheema, Hafiza M N Khan, Iqrar A Khan, Asif A Ahmad, Sheraz Malik, Waqas |
AuthorAffiliation | 2 Genomics Lab, Department of Plant Breeding and Genetics, Bahauddin Zakariya University Multan, Pakistan 1 Plant Genetic Resources Lab, Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan |
AuthorAffiliation_xml | – name: 2 Genomics Lab, Department of Plant Breeding and Genetics, Bahauddin Zakariya University Multan, Pakistan – name: 1 Plant Genetic Resources Lab, Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan |
Author_xml | – sequence: 1 givenname: Usman surname: Aslam fullname: Aslam, Usman organization: Plant Genetic Resources Lab, Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan – sequence: 2 givenname: Hafiza M N surname: Cheema fullname: Cheema, Hafiza M N organization: Plant Genetic Resources Lab, Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan – sequence: 3 givenname: Sheraz surname: Ahmad fullname: Ahmad, Sheraz organization: Plant Genetic Resources Lab, Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan – sequence: 4 givenname: Iqrar A surname: Khan fullname: Khan, Iqrar A organization: Plant Genetic Resources Lab, Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan – sequence: 5 givenname: Waqas surname: Malik fullname: Malik, Waqas organization: Genomics Lab, Department of Plant Breeding and Genetics, Bahauddin Zakariya University Multan, Pakistan – sequence: 6 givenname: Asif A surname: Khan fullname: Khan, Asif A organization: Plant Genetic Resources Lab, Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28082993$$D View this record in MEDLINE/PubMed |
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CitedBy_id | crossref_primary_10_2139_ssrn_4108593 crossref_primary_10_1007_s10681_019_2510_6 crossref_primary_10_1007_s00438_018_1482_x crossref_primary_10_1111_tpj_14755 crossref_primary_10_1007_s10681_018_2206_3 crossref_primary_10_3389_fpls_2018_00216 crossref_primary_10_1080_07388551_2023_2231630 crossref_primary_10_3389_fmars_2023_1166370 crossref_primary_10_1007_s42535_022_00385_4 crossref_primary_10_3389_fpls_2021_730718 |
Cites_doi | 10.1101/gr.2673204 10.1007/978-1-4020-6295-7_15 10.1104/pp.123.2.439 10.1111/j.1467-7652.2008.00341.x 10.1038/nbt1043 10.1186/gb-2008-9-2-r43 10.2307/3869192 10.1093/aob/mci078 10.1104/pp.118.2.399 10.1186/1471-2229-7-19 10.1007/BF01276783 10.1186/1756-0500-2-141 10.1186/1471-2164-15-838 10.1093/genetics/164.2.731 10.1186/1471-2229-11-81 10.1002/jsfa.7120 10.1186/1472-6750-7-29 10.1371/journal.pone.0041570 10.1186/1471-2229-10-62 10.1186/1756-0500-3-69 10.1007/s11032-006-9046-x 10.1038/nprot.2006.329 10.1186/1471-2229-4-12 10.2135/cropsci2002.0011 10.1126/science.1086391 10.1186/1746-4811-7-20 10.1105/tpc.010108 10.1186/1471-2229-13-38 10.1038/nrg1711 10.1046/j.1365-313X.2001.01105.x 10.1186/1471-2229-8-9 10.1016/S0065-2113(02)78004-8 10.1007/BF00194429 10.1093/pcp/pcr134 10.1186/1471-2229-9-115 10.1104/pp.102.017384 |
ContentType | Journal Article |
Copyright | Copyright © 2016 Aslam, Cheema, Ahmad, Khan, Malik and Khan. 2016 Aslam, Cheema, Ahmad, Khan, Malik and Khan |
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Keywords | RGAs EMS mutagenesis TILLING Gossypium hirsutum fiber quality point mutation |
Language | English |
License | This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Frikkie C. Botha, Sugar Research Australia, Australia This article was submitted to Plant Biotechnology, a section of the journal Frontiers in Plant Science Reviewed by: Zhanguo Xin, Agricultural Research Service (USDA), USA; Bert Collard, Sugar Research Australia, Australia |
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References | 12807792 - Genetics. 2003 Jun;164(2):731-40 9765525 - Plant Physiol. 1998 Oct;118(2):399-406 23496999 - BMC Plant Biol. 2013 Mar 05;13:38 22844501 - PLoS One. 2012;7(7):e41570 20380715 - BMC Plant Biol. 2010 Apr 09;10:62 12644638 - Plant Physiol. 2003 Mar;131(3):866-71 15701660 - Ann Bot. 2005 Apr;95(5):789-97 17543120 - BMC Biotechnol. 2007 Jun 01;7:29 12893945 - Science. 2003 Aug 1;301(5633):653-7 15256507 - Genome Res. 2004 Aug;14(8):1474-82 25640313 - J Sci Food Agric. 2016 Jan 30;96(2):530-8 24178489 - Planta. 1993 Mar;189(3):329-39 18218134 - BMC Plant Biol. 2008 Jan 24;8:9 12324607 - Plant Cell. 1991 Jun;3(6):637-644 12671090 - Plant Cell. 2003 Apr;15(4):952-64 21965606 - Plant Cell Physiol. 2011 Nov;52(11):1994-2005 17406493 - Nat Protoc. 2006;1(5):2465-77 21718494 - Plant Methods. 2011 Jun 30;7(1):20 15580263 - Nat Biotechnol. 2005 Jan;23(1):75-81 11576441 - Plant J. 2001 Sep;27(6):581-90 20222995 - BMC Res Notes. 2010 Mar 12;3:69 19615043 - BMC Res Notes. 2009 Jul 17;2:141 17428339 - BMC Plant Biol. 2007 Apr 11;7:19 21569438 - BMC Plant Biol. 2011 May 12;11:81 25273845 - BMC Genomics. 2014 Oct 02;15:838 19712486 - BMC Plant Biol. 2009 Aug 28;9:115 10859174 - Plant Physiol. 2000 Jun;123(2):439-42 15282033 - BMC Plant Biol. 2004 Jul 28;4:12 16304599 - Nat Rev Genet. 2005 Nov;6(11):836-46 11756248 - Crop Sci. 2002 Jan;42(1):11-20 18422888 - Plant Biotechnol J. 2008 Jun;6(5):477-85 18302733 - Genome Biol. 2008;9(2):R43 Khan (B13) 2016; 96 Stephenson (B28) 2010; 10 Chen (B3) 2012; 7 Till (B33) 2006; 1 Kumar (B15) 2013; 13 Ruan (B22) 1998; 118 Cooper (B5) 2008; 8 Grover (B11) 2004; 14 Pimkin (B20) 2007; 7 Aslam (B2) 2013; 15 Yan (B37) 2002; 42 Talamè (B29) 2008; 6 Hendrix (B12) 2005; 95 Wendel (B35) 2003; 78 Okabe (B18) 2011; 52 Staiger (B26) 1991; 3 Comai (B4) 2005; 6 Till (B30) 2007a Ruan (B23) 2003; 15 Minoia (B17) 2010; 3 Slade (B25) 2005; 23 Till (B31) 2007b; 7 Alonso (B1) 2003; 301 Uauy (B34) 2009; 9 Knoll (B14) 2011; 11 McCallum (B16) 2000; 123 Greene (B10) 2003; 164 Sabetta (B24) 2011; 7 Wesley (B36) 2001; 27 Dalmais (B6) 2008; 9 Elias (B7) 2009; 2 Till (B32) 2004; 4 Perry (B19) 2003; 131 Raghavan (B21) 2007; 19 Geigenberger (B9) 1993; 189 Fang (B8) 2014; 15 Staiger (B27) 1987; 141 |
References_xml | – volume: 14 start-page: 1474 year: 2004 ident: B11 article-title: Incongruent patterns of local and global genome size evolution in cotton. publication-title: Genome Res. doi: 10.1101/gr.2673204 contributor: fullname: Grover – start-page: 333 year: 2007a ident: B30 publication-title: “TILLING and Ecotilling for Crop Improvement,” in Genomics-Assisted Crop Improvement. doi: 10.1007/978-1-4020-6295-7_15 contributor: fullname: Till – volume: 123 start-page: 439 year: 2000 ident: B16 article-title: Targeting induced local lesions IN genomes (TILLING) for plant functional genomics. publication-title: Plant Physiol. doi: 10.1104/pp.123.2.439 contributor: fullname: McCallum – volume: 6 start-page: 477 year: 2008 ident: B29 article-title: TILLMore, a resource for the discovery of chemically induced mutants in barley. publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2008.00341.x contributor: fullname: Talamè – volume: 23 start-page: 75 year: 2005 ident: B25 article-title: A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING. publication-title: Nat. Biotechnol. doi: 10.1038/nbt1043 contributor: fullname: Slade – volume: 9 issue: R43 year: 2008 ident: B6 article-title: UTILLdb, a Pisum sativum in silico forward and reverse genetics tool. publication-title: Genome Biol. doi: 10.1186/gb-2008-9-2-r43 contributor: fullname: Dalmais – volume: 3 start-page: 637 year: 1991 ident: B26 article-title: Microfilament distribution in maize meiotic mutants correlates with microtubule organization. publication-title: Plant Cell doi: 10.2307/3869192 contributor: fullname: Staiger – volume: 95 start-page: 789 year: 2005 ident: B12 article-title: Estimation of the nuclear DNA content of Gossypium species. publication-title: Ann. Bot. doi: 10.1093/aob/mci078 contributor: fullname: Hendrix – volume: 118 start-page: 399 year: 1998 ident: B22 article-title: A fiberless seed mutation in cotton is associated with lack of fiber cell initiation in ovule epidermis and alterations in sucrose synthase expression and carbon partitioning in developing seeds. publication-title: Plant Physiol. doi: 10.1104/pp.118.2.399 contributor: fullname: Ruan – volume: 7 issue: 19 year: 2007b ident: B31 article-title: Discovery of chemically induced mutations in rice by TILLING. publication-title: BMC Plant Biol. doi: 10.1186/1471-2229-7-19 contributor: fullname: Till – volume: 141 start-page: 1 year: 1987 ident: B27 article-title: Actin localization and function in higher plants. publication-title: Protoplasma doi: 10.1007/BF01276783 contributor: fullname: Staiger – volume: 2 issue: 141 year: 2009 ident: B7 article-title: Optimizing TILLING and Ecotilling techniques for potato (Solanum tuberosum L). publication-title: BMC Res. Notes doi: 10.1186/1756-0500-2-141 contributor: fullname: Elias – volume: 15 issue: 838 year: 2014 ident: B8 article-title: Cotton fiber elongation network revealed by expression profiling of longer fiber lines introgressed with different Gossypium barbadense chromosome segments. publication-title: BMC Genomics doi: 10.1186/1471-2164-15-838 contributor: fullname: Fang – volume: 164 start-page: 731 year: 2003 ident: B10 article-title: Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. publication-title: Genetics doi: 10.1093/genetics/164.2.731 contributor: fullname: Greene – volume: 11 issue: 81 year: 2011 ident: B14 article-title: TILLING for allergen reduction and improvement of quality traits in peanut (Arachis hypogaea L.). publication-title: BMC Plant Biol. doi: 10.1186/1471-2229-11-81 contributor: fullname: Knoll – volume: 96 start-page: 530 year: 2016 ident: B13 article-title: Comparative analysis of resistance gene analogues encoding NBS-LRR domains in cotton. publication-title: J. Sci. Food Agric. doi: 10.1002/jsfa.7120 contributor: fullname: Khan – volume: 7 issue: 29 year: 2007 ident: B20 article-title: Recombinant nucleases CEL I from celery and SP I from spinach for mutation detection. publication-title: BMC Biotechnol. doi: 10.1186/1472-6750-7-29 contributor: fullname: Pimkin – volume: 7 issue: e41570 year: 2012 ident: B3 article-title: Development and characterization of a new TILLING population of common bread wheat (Triticum aestivum L.). publication-title: PLoS ONE doi: 10.1371/journal.pone.0041570 contributor: fullname: Chen – volume: 10 issue: 62 year: 2010 ident: B28 article-title: A rich TILLING resource for studying gene function in Brassica rapa. publication-title: BMC Plant Biol. doi: 10.1186/1471-2229-10-62 contributor: fullname: Stephenson – volume: 15 start-page: 11 year: 2013 ident: B2 article-title: Kill curve analysis and response of ethyl methanesulfonate and γ-rays in diploid and tetraploid cotton. publication-title: Int. J. Agric. Biol. contributor: fullname: Aslam – volume: 3 issue: 69 year: 2010 ident: B17 article-title: A new mutant genetic resource for tomato crop improvement by TILLING technology. publication-title: BMC Res. Notes doi: 10.1186/1756-0500-3-69 contributor: fullname: Minoia – volume: 19 start-page: 87 year: 2007 ident: B21 article-title: Rapid method for detecting SNPs on agarose gels and its application in candidate gene mapping. publication-title: Mol. Breed. doi: 10.1007/s11032-006-9046-x contributor: fullname: Raghavan – volume: 1 start-page: 2465 year: 2006 ident: B33 article-title: A protocol for TILLING and Ecotilling in plants and animals. publication-title: Nat. Protoc. doi: 10.1038/nprot.2006.329 contributor: fullname: Till – volume: 4 issue: 12 year: 2004 ident: B32 article-title: Discovery of induced point mutations in maize genes by TILLING. publication-title: BMC Plant Biol. doi: 10.1186/1471-2229-4-12 contributor: fullname: Till – volume: 42 start-page: 11 year: 2002 ident: B37 article-title: Biplot analysis of test sites and trait relations of soybean in ontario. publication-title: Crop Sci. doi: 10.2135/cropsci2002.0011 contributor: fullname: Yan – volume: 301 start-page: 653 year: 2003 ident: B1 article-title: Genome-wide insertional mutagenesis of Arabidopsis thaliana. publication-title: Science doi: 10.1126/science.1086391 contributor: fullname: Alonso – volume: 7 issue: 20 year: 2011 ident: B24 article-title: sunTILL: a TILLING resource for gene function analysis in sunflower. publication-title: Plant Methods doi: 10.1186/1746-4811-7-20 contributor: fullname: Sabetta – volume: 15 start-page: 952 year: 2003 ident: B23 article-title: Suppression of sucrose synthase gene expression represses cotton fiber cell initiation, elongation, and seed development. publication-title: Plant Cell doi: 10.1105/tpc.010108 contributor: fullname: Ruan – volume: 13 issue: 38 year: 2013 ident: B15 article-title: SMART–sunflower mutant population and reverse genetic tool for crop improvement. publication-title: BMC Plant Biol. doi: 10.1186/1471-2229-13-38 contributor: fullname: Kumar – volume: 6 start-page: 836 year: 2005 ident: B4 article-title: The advantages and disadvantages of being polyploid. publication-title: Nat. Rev. Genet. doi: 10.1038/nrg1711 contributor: fullname: Comai – volume: 27 start-page: 581 year: 2001 ident: B36 article-title: Construct design for efficient, effective and high-throughput gene silencing in plants. publication-title: Plant J. doi: 10.1046/j.1365-313X.2001.01105.x contributor: fullname: Wesley – volume: 8 issue: 9 year: 2008 ident: B5 article-title: TILLING to detect induced mutations in soybean. publication-title: BMC Plant Biol. doi: 10.1186/1471-2229-8-9 contributor: fullname: Cooper – volume: 78 start-page: 139 year: 2003 ident: B35 article-title: Polyploidy and the evolutionary history of cotton. publication-title: Adv. Agron. doi: 10.1016/S0065-2113(02)78004-8 contributor: fullname: Wendel – volume: 189 start-page: 329 year: 1993 ident: B9 article-title: Sucrose synthase catalyses a readily reversible reaction in vivo in developing potato tubers and other plant tissues. publication-title: Planta doi: 10.1007/BF00194429 contributor: fullname: Geigenberger – volume: 52 start-page: 1994 year: 2011 ident: B18 article-title: Tomato TILLING technology: development of a reverse genetics tool for the efficient isolation of mutants from micro-tom mutant libraries. publication-title: Plant Cell Physiol. doi: 10.1093/pcp/pcr134 contributor: fullname: Okabe – volume: 9 issue: 115 year: 2009 ident: B34 article-title: A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat. publication-title: BMC Plant Biol. doi: 10.1186/1471-2229-9-115 contributor: fullname: Uauy – volume: 131 start-page: 866 year: 2003 ident: B19 article-title: A TILLING reverse genetics tool and a web-accessible collection of mutants of the legume Lotus japonicus. publication-title: Plant Physiol. doi: 10.1104/pp.102.017384 contributor: fullname: Perry |
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Snippet | Cotton is cultivated worldwide for its white fiber, of which around 90% is tetraploid upland cotton (
L.) carrying both A and D genome. Since centuries, yield... Cotton is cultivated worldwide for its white fiber, of which around 90% is tetraploid upland cotton ( Gossypium hirsutum L.) carrying both A and D genome.... |
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StartPage | 1863 |
SubjectTerms | Plant Science |
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Title | COTIP: Cotton TILLING Platform, a Resource for Plant Improvement and Reverse Genetic Studies |
URI | https://www.ncbi.nlm.nih.gov/pubmed/28082993 https://search.proquest.com/docview/1861488458 https://pubmed.ncbi.nlm.nih.gov/PMC5183611 |
Volume | 7 |
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