Genomic Characterization of 16S rRNA Methyltransferase-Producing Enterobacterales Reveals the Emergence of Klebsiella pneumoniae ST6260 Harboring rmtF, rmtB, blaNDM-5, blaOXA-232 and blaSFO-1 Genes in a Cancer Hospital in Bulgaria

• Published in: Antibiotics (Basel) Vol. 13; no. 10; p. 950
• Main Authors: Sabtcheva, Stefana, Stoikov, Ivan, Georgieva, Sylvia, Donchev, Deyan, Hodzhev, Yordan, Dobreva, Elina, Christova, Iva, Ivanov, Ivan N.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 10.10.2024; MDPI
• Subjects: 16S rRNA methyltransferase; Amides; aminoglycoside resistance; Aminoglycosides; Antibiotic resistance; Antibiotics; ArmA; Bacterial infections; Carbapenemase; Coexistence; Conjugation; Drug resistance; E coli; Enterobacterales; Enzymes; Gene sequencing; Genes; Genomics; Gram-negative bacteria; Health risks; Klebsiella; Multidrug resistance; Plasmids; Public health; Quinolones; RmtB; RmtF; rRNA (adenosine-2'-0'-)-methyltransferase; rRNA 16S; rRNA methyltransferase; Surveillance; Whole genome sequencing; β Lactamase; β-Lactam antibiotics; Bulgaria; Japan

Background: Acquired 16S rRNA methyltransferases (16S-RMTases) confer high-level resistance to aminoglycosides and are often associated with β-lactam and quinolone resistance determinants. Methods: Using PCR, whole-genome sequencing and conjugation experiments, we conducted a retrospective genomic s...