Methods for Measuring the Concentrations of Proteins

• Published in: Cold Spring Harbor protocols Vol. 2020; no. 4; p. pdb.top102277
• Main Authors: Kielkopf, Clara L, Bauer, William, Urbatsch, Ina L
• Format: Journal Article
• Language: English
• Published: United States 01.04.2020

Determining the concentration of protein samples generally is accomplished either by measuring the UV absorbance at 280 nm or by reacting the protein quantitatively with dyes and/or metal ions (Bradford, Lowry, or BCA assays). For purified proteins, UV absorbance remains the most popular method beca...