Stimulation of tobacco shoot regeneration by alternating weak electric field

Nicotiana tabacum morphogenetic callus was used to study the stimulative effects of external alternating electric currents of low level and low frequency on the cytodifferentiation processes. The N. tabacum calli were grown on standard medium for shoot regeneration and subjected to electric currents...

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Published inBioelectrochemistry and Bioenergetics Vol. 44; no. 2; pp. 257 - 260
Main Authors Cogalniceanu, Gina, Radu, M, Fologea, D, Moisoi, Nicoleta, Aurelia Brezeanu
Format Journal Article
LanguageEnglish
Published Elsevier S.A 1998
Subjects
Alternating electric field
Electrostimulation
In vitro shoot regeneration
Tobacco
Tobacco
In vitro shoot regeneration
Electrostimulation
Alternating electric field
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Abstract Nicotiana tabacum morphogenetic callus was used to study the stimulative effects of external alternating electric currents of low level and low frequency on the cytodifferentiation processes. The N. tabacum calli were grown on standard medium for shoot regeneration and subjected to electric currents (50 Hz, 0.1–50 μA) during the whole period of treatment (30 days). The number of shoots, total mass, DNA and protein content were measured for each callus sample at the end of the experiment. The number of shoots increased by up to 300% for the samples stimulated with 50 μA although no significant changes were noted in total mass, DNA and protein content in the stimulated samples compared to control. We suggest that in the presence of the external electric current, the callus cells become more sensitive to chemical signals (hormones and/or ions) in the culture medium. A series of changes in the activity of membrane components, as a consequence of the modulation of membrane potential by the external electric field, could support our hypothesis.
AbstractList Nicotiana tabacum morphogenetic callus was used to study the stimulative effects of external alternating electric currents of low level and low frequency on the cytodifferentiation processes. The N. tabacum calli were grown on standard medium for shoot regeneration and subjected to electric currents (50 Hz, 0.1–50 μA) during the whole period of treatment (30 days). The number of shoots, total mass, DNA and protein content were measured for each callus sample at the end of the experiment. The number of shoots increased by up to 300% for the samples stimulated with 50 μA although no significant changes were noted in total mass, DNA and protein content in the stimulated samples compared to control. We suggest that in the presence of the external electric current, the callus cells become more sensitive to chemical signals (hormones and/or ions) in the culture medium. A series of changes in the activity of membrane components, as a consequence of the modulation of membrane potential by the external electric field, could support our hypothesis.
Author Aurelia Brezeanu
Fologea, D
Radu, M
Cogalniceanu, Gina
Moisoi, Nicoleta
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  organization: Institute of Biology, Romanian Academy of Science, Department of Plant Morphogenesis and Genetic Engineering, Spl. Independentei 296, P.O. Box 56-53, 79651, Bucharest, Romania
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10.3109/15368379209012850
10.1016/0302-4598(93)86102-7
10.1111/j.1399-3054.1962.tb08052.x
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Alternating electric field
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SubjectTerms Alternating electric field
Electrostimulation
In vitro shoot regeneration
Tobacco
Title Stimulation of tobacco shoot regeneration by alternating weak electric field
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