Characterization of the NADPH-Dependent Metabolism of 17β-Estradiol to Multiple Metabolites by Human Liver Microsomes and Selectively Expressed Human Cytochrome P450 3A4 and 3A5

• Published in: The Journal of pharmacology and experimental therapeutics Vol. 298; no. 2; pp. 420 - 432
• Main Authors: Lee, Anthony J., Kosh, Joseph W., Conney, Allan H., Zhu, Bao Ting
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.08.2001
• Subjects: NADPH; BSTFA; OH; CYP; TMS; E1; E2; E3; HPLC; GC/MS; TMCS

We characterized the NADPH-dependent metabolism of 17β-estradiol (E2) by liver microsomes from 21 male and 12 female human subjects. A large number of radioactive estrogen metabolite peaks were detected following incubations of [3H]E2 with male or female human liver microsomes in the presence of NAD...