Measurement of circulating biliary antigen-antibody immune complex in the patients of primary biliary cirrhosis using ELISA
We purified the hepatic bile duct antigen from the human bile and confirmed that this antigen showed the antigenic cross reactivity with salivary gland duct, pancreatic duct and renal tubulus, but no cross reactivity with other organs. And we prepared the species specific antibody (bile duct antibod...
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| Published in | Kanzo Vol. 27; no. 6; pp. 746 - 752 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
The Japan Society of Hepatology
1986
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| Online Access | Get full text |
| ISSN | 0451-4203 1881-3593 |
| DOI | 10.2957/kanzo.27.746 |
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| Abstract | We purified the hepatic bile duct antigen from the human bile and confirmed that this antigen showed the antigenic cross reactivity with salivary gland duct, pancreatic duct and renal tubulus, but no cross reactivity with other organs. And we prepared the species specific antibody (bile duct antibody) against the bile antigen in rabbits to detect the bile antibody in circulating immune complex from the patients with PBC by using ELISA. In PBC, 20 of 68 cases showed positive results (29.4%). In contrast, bile antibody was detected in none of 3 cases with lupoid hepatitis, 1 of 4 SLE, 1 of 6 rheumatoid arthritis, 1 of 10 Sjogren's syndrome which is often associated with PBC, and 2 of 36 chronic pancreatitis. The present study showed that bile antibody was contained frequently within circulating immune complex from patients with PBC. These findings support that PBC is likely to be a autoimmune disease. |
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| AbstractList | We purified the hepatic bile duct antigen from the human bile and confirmed that this antigen showed the antigenic cross reactivity with salivary gland duct, pancreatic duct and renal tubulus, but no cross reactivity with other organs. And we prepared the species specific antibody (bile duct antibody) against the bile antigen in rabbits to detect the bile antibody in circulating immune complex from the patients with PBC by using ELISA. In PBC, 20 of 68 cases showed positive results (29.4%). In contrast, bile antibody was detected in none of 3 cases with lupoid hepatitis, 1 of 4 SLE, 1 of 6 rheumatoid arthritis, 1 of 10 Sjogren's syndrome which is often associated with PBC, and 2 of 36 chronic pancreatitis. The present study showed that bile antibody was contained frequently within circulating immune complex from patients with PBC. These findings support that PBC is likely to be a autoimmune disease. |
| Author | MIYAZAKI, Masako OKINO, Minoru YAMAMOTO, Yasuro OONISHI, Saburo |
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| References | 4) Amoroso P, Eddleston ALWF, Williams R,: Identification of biliary antigens in circulating immune complexes in primary biliary cirrhosis. Clin Exp Immunol 42: 95-98, 1980 14) Culp KS, Fleming CR, Dickson ER,: Autoimmune associations in primary biliary cirrhosis. Mayo Clin Proc 57: 365-370, 1982 1) McFarlane IG, Eddleston ALWF, Williams R,: Leukocyte migration inhibition in response to biliary cirrhosis, sclerosing cholangitis and other chronic liver diseases. Gastroenterology 76: 1333, 1979 6) 佐々木博:原発性胆汁性肝硬変(PBC)診断基準.厚生省特定疾患難治性の肝炎調査研究班,昭和56年度研究報告,p152 13) 佐々木博,井上恭一,樋口清博,他:原発性胆汁性肝硬変の全国統計.肝胆膵4: 171-178, 1982 7) 大西三朗,山本泰朗:原発性胆汁性肝硬変症における肝内胆管に対する特異抗体の役割と免疫学的調節.「肝と免疫-免疫性肝障害を中心に」,長島秀夫,浪久利彦,山本祐夫編,医歯薬出版,東京,1984,p108-114 11) Roitt IM: Autoimmunity. In: Essential Immunology. 2nd ed, Blackwell Scientific Publications. Oxford 1974, P211-241 16) 木谷恒,澤武紀雄,服部信,他:原発性胆汁性肝硬変における膵病変について.日消誌81: 2544-2551, 1984 8) 伊藤憲一,山本泰朗,大西三朗:肝内胆汁うっ滞の病態成立における免疫の関与.「肝の生化学=箱根シンポジウムI」,箱根シンポジウム記録刊行会編,中外医学社,東京,1985, p162-171 15) Pares A, Rimola A, Rodes J,: Renal tubular acidosis in primary biliary cirrhosis. Gastroenterology 80: 681-686, 1981 12) Sullivan S, Eddleston ALWF, Williams R,: Sicca syndrome and immune response to bile and salivary antigens. In: Immune reactions in liver disease, Edited by ALWF Eddleston, JCP Weber, R Williams, Pitman Medical Publishing Co. Ltd, London, 1979, p144-148 19) Epstein O, Thomas HC, Sherlock S,: Primary biliary cirrhosis is a dry gland syndrom with features of chronic graft-versushost disease. Lancet I: 1166-1168, 1980 21) 佐野萬瑳寿,足立正彦,伊藤憲一,他:胆汁中に存在する胆管上皮抗原の検索.肝臓19: 835-839, 1978 17) Epstein O, Chapman RWG, Sherlock S,: The pancreas in primary biliary cirrhosis and primary sclerosing cholangitis. Gastroenterology 83: 1177-1182, 1982 2) 竹内孝男,佐野萬瑳寿,伊藤憲一,他:慢性肝疾患における自己抗原に対する細胞性免疫に関する研究.第2編.原発性胆汁性肝硬変症における胆管上皮抗原に対する白血球遊走阻止試験.日消誌75: 1031-1035, 1978 3) 福田善弘,佐野萬瑳寿,伊藤憲一,他:原発性胆汁性肝硬変症における免疫複合体の検索.肝胆膵4: 613-618, 1982 20) Klöppel G, Sack HJ, Berg PA,: Histopatlological features in mixed types of chronic aggressive hepatitis and primary biliary cirr-hosis. Virchow Arch 373: 143-160. 1977 5) 山本泰朗,岡崎和一,伊藤憲一,他:原発性胆汁性肝硬変症における血中胆管上皮抗原・抗体複合物の検索.「消化器と免疫No.9」,戸部隆吉,斎藤和久,濱島義博,他編,医歯薬出版,東京,1982,p90-93 9) Witebsky E, Rose NR, Paine JR,: Chronic thyroiditis and autoimmunization. JAMA 164: 1439-1447, 1957 18) 山崎潔,佐藤俊一,中沢一臣,他:Sjögren症候群を合併した原発性胆汁性肝硬変症の1例.肝臓25: 1461-1467, 1984 10) Mackay IR: Autoimmune apects of systemic lupus erythematosus and the forbidden-clone hypothesis. In: Lupus erythematosus, Edited by EL Dubois, MacGraw-Hill Inc. New York, 1974, p142-152 |
| References_xml | – reference: 1) McFarlane IG, Eddleston ALWF, Williams R,: Leukocyte migration inhibition in response to biliary cirrhosis, sclerosing cholangitis and other chronic liver diseases. Gastroenterology 76: 1333, 1979 – reference: 17) Epstein O, Chapman RWG, Sherlock S,: The pancreas in primary biliary cirrhosis and primary sclerosing cholangitis. Gastroenterology 83: 1177-1182, 1982 – reference: 21) 佐野萬瑳寿,足立正彦,伊藤憲一,他:胆汁中に存在する胆管上皮抗原の検索.肝臓19: 835-839, 1978 – reference: 8) 伊藤憲一,山本泰朗,大西三朗:肝内胆汁うっ滞の病態成立における免疫の関与.「肝の生化学=箱根シンポジウムI」,箱根シンポジウム記録刊行会編,中外医学社,東京,1985, p162-171 – reference: 7) 大西三朗,山本泰朗:原発性胆汁性肝硬変症における肝内胆管に対する特異抗体の役割と免疫学的調節.「肝と免疫-免疫性肝障害を中心に」,長島秀夫,浪久利彦,山本祐夫編,医歯薬出版,東京,1984,p108-114 – reference: 13) 佐々木博,井上恭一,樋口清博,他:原発性胆汁性肝硬変の全国統計.肝胆膵4: 171-178, 1982 – reference: 10) Mackay IR: Autoimmune apects of systemic lupus erythematosus and the forbidden-clone hypothesis. In: Lupus erythematosus, Edited by EL Dubois, MacGraw-Hill Inc. New York, 1974, p142-152 – reference: 16) 木谷恒,澤武紀雄,服部信,他:原発性胆汁性肝硬変における膵病変について.日消誌81: 2544-2551, 1984 – reference: 15) Pares A, Rimola A, Rodes J,: Renal tubular acidosis in primary biliary cirrhosis. Gastroenterology 80: 681-686, 1981 – reference: 5) 山本泰朗,岡崎和一,伊藤憲一,他:原発性胆汁性肝硬変症における血中胆管上皮抗原・抗体複合物の検索.「消化器と免疫No.9」,戸部隆吉,斎藤和久,濱島義博,他編,医歯薬出版,東京,1982,p90-93 – reference: 18) 山崎潔,佐藤俊一,中沢一臣,他:Sjögren症候群を合併した原発性胆汁性肝硬変症の1例.肝臓25: 1461-1467, 1984 – reference: 12) Sullivan S, Eddleston ALWF, Williams R,: Sicca syndrome and immune response to bile and salivary antigens. In: Immune reactions in liver disease, Edited by ALWF Eddleston, JCP Weber, R Williams, Pitman Medical Publishing Co. Ltd, London, 1979, p144-148 – reference: 9) Witebsky E, Rose NR, Paine JR,: Chronic thyroiditis and autoimmunization. JAMA 164: 1439-1447, 1957 – reference: 20) Klöppel G, Sack HJ, Berg PA,: Histopatlological features in mixed types of chronic aggressive hepatitis and primary biliary cirr-hosis. Virchow Arch 373: 143-160. 1977 – reference: 3) 福田善弘,佐野萬瑳寿,伊藤憲一,他:原発性胆汁性肝硬変症における免疫複合体の検索.肝胆膵4: 613-618, 1982 – reference: 6) 佐々木博:原発性胆汁性肝硬変(PBC)診断基準.厚生省特定疾患難治性の肝炎調査研究班,昭和56年度研究報告,p152 – reference: 11) Roitt IM: Autoimmunity. In: Essential Immunology. 2nd ed, Blackwell Scientific Publications. Oxford 1974, P211-241 – reference: 4) Amoroso P, Eddleston ALWF, Williams R,: Identification of biliary antigens in circulating immune complexes in primary biliary cirrhosis. Clin Exp Immunol 42: 95-98, 1980 – reference: 19) Epstein O, Thomas HC, Sherlock S,: Primary biliary cirrhosis is a dry gland syndrom with features of chronic graft-versushost disease. Lancet I: 1166-1168, 1980 – reference: 14) Culp KS, Fleming CR, Dickson ER,: Autoimmune associations in primary biliary cirrhosis. Mayo Clin Proc 57: 365-370, 1982 – reference: 2) 竹内孝男,佐野萬瑳寿,伊藤憲一,他:慢性肝疾患における自己抗原に対する細胞性免疫に関する研究.第2編.原発性胆汁性肝硬変症における胆管上皮抗原に対する白血球遊走阻止試験.日消誌75: 1031-1035, 1978 |
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| Title | Measurement of circulating biliary antigen-antibody immune complex in the patients of primary biliary cirrhosis using ELISA |
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