Novel approach of amplification and cloning of bacterial cellulose synthesis (bcs) operon from Gluconoacetobacter hansenii
This study aimed to assess a novel approach for amplifying and molecular cloning of the four genes encoding bacterial cellulose synthase (bcs-ABCD). The four bcs-encoding genes were directly amplified from genomic DNA extracted from the wild type Gluconoacetobacter hansenii NRRL 1034 in two PCR roun...
Saved in:
Published in | Gene reports Vol. 27; p. 101577 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Inc
01.06.2022
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | This study aimed to assess a novel approach for amplifying and molecular cloning of the four genes encoding bacterial cellulose synthase (bcs-ABCD).
The four bcs-encoding genes were directly amplified from genomic DNA extracted from the wild type Gluconoacetobacter hansenii NRRL 1034 in two PCR rounds.
The target genes were successfully amplified at the expected molecular weight and further confirmed by results of blast analysis that revealed 99% homology of both nucleotide and amino acid sequences of bcs amplicons with a previously published sequence of Gluconacetobacter xylinus.
The newly proposed approach proved efficient as proved by successful amplification/cloning of the four genes of BC-encoding operon without any structural changes that might affect their expected functions. This approach overcomes the limitations associated with the absence of vector-compatible restriction sites in the vicinity of the bsc cassette and provides more opportunities for cloning or expression using any commercial vector.
•This report proposes a novel approach for bacterial cellulose synthase (bcs-ABCD) amplification and molecular cloning.•The newly proposed approach proved efficient as proved by successful amplification/cloning of the four genes of BC-encoding operon without any structural changes as revealed by blast results showing 99% homology with bcs-ABCD published sequences.•This method of amplification overcomes the limitations associated with the absence of vector-compatible restriction sites in the vicinity of the bsc cassette and provides more opportunities for cloning or expression using any commercial vector. |
---|---|
AbstractList | This study aimed to assess a novel approach for amplifying and molecular cloning of the four genes encoding bacterial cellulose synthase (bcs-ABCD).
The four bcs-encoding genes were directly amplified from genomic DNA extracted from the wild type Gluconoacetobacter hansenii NRRL 1034 in two PCR rounds.
The target genes were successfully amplified at the expected molecular weight and further confirmed by results of blast analysis that revealed 99% homology of both nucleotide and amino acid sequences of bcs amplicons with a previously published sequence of Gluconacetobacter xylinus.
The newly proposed approach proved efficient as proved by successful amplification/cloning of the four genes of BC-encoding operon without any structural changes that might affect their expected functions. This approach overcomes the limitations associated with the absence of vector-compatible restriction sites in the vicinity of the bsc cassette and provides more opportunities for cloning or expression using any commercial vector.
•This report proposes a novel approach for bacterial cellulose synthase (bcs-ABCD) amplification and molecular cloning.•The newly proposed approach proved efficient as proved by successful amplification/cloning of the four genes of BC-encoding operon without any structural changes as revealed by blast results showing 99% homology with bcs-ABCD published sequences.•This method of amplification overcomes the limitations associated with the absence of vector-compatible restriction sites in the vicinity of the bsc cassette and provides more opportunities for cloning or expression using any commercial vector. |
ArticleNumber | 101577 |
Author | El-Ghwas, Dina E. Al-Janabi, Saif S. Farrag, Ayman A. El-Waseif, Amr A. Abdelghany, Tarek M. Shawky, Heba |
Author_xml | – sequence: 1 givenname: Saif S. surname: Al-Janabi fullname: Al-Janabi, Saif S. email: saif.saad@uoa.edu.iq organization: Botany and Microbiology Dept., Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt – sequence: 2 givenname: Heba surname: Shawky fullname: Shawky, Heba email: hs.tohami@nrc.sci.eg organization: Therapeutic Chemistry Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, 12622 Cairo, Egypt – sequence: 3 givenname: Amr A. surname: El-Waseif fullname: El-Waseif, Amr A. email: amrelwaseif@azhar.edu.eg organization: Botany and Microbiology Dept., Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt – sequence: 4 givenname: Ayman A. surname: Farrag fullname: Farrag, Ayman A. organization: Botany and Microbiology Dept., Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt – sequence: 5 givenname: Tarek M. surname: Abdelghany fullname: Abdelghany, Tarek M. email: tabdelghany.201@azhar.edu.eg organization: Botany and Microbiology Dept., Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt – sequence: 6 givenname: Dina E. surname: El-Ghwas fullname: El-Ghwas, Dina E. organization: Chemistry of Natural and Microbial products Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, 12622 Cairo, Egypt |
BookMark | eNp9kLFOwzAQhi1UJErpGzB4hCHFdpy4WZBQBQWpgqW75Tjn1pVrR3ZaqTw9icLAxHSnO32_7r5bNPHBA0L3lCwooeXTYbEDH6FdMMLYMCqEuEJTxguWEcr55E9_g-YpHQjpOUErwafo-zOcwWHVtjEovcfBYHVsnTVWq84Gj5VvsHbBW78blrXSHUSrHNbg3MmFBDhdfLeHZBN-qHV6xKGF2JMmhiNeu5MOvo-GLows3iufwFt7h66Ncgnmv3WGtm-v29V7tvlaf6xeNpnOSdlldVVWDRVV3QhjmOEl5RVjvCo5yYHlmtV5v6-pMJo2OVkyQ8ti2f-oiqUqinyG-BirY0gpgpFttEcVL5ISORiUBzkalINBORrssecRg_60s4Uok7bgNTQ2gu5kE-z_AT-ZWn7c |
CitedBy_id | crossref_primary_10_1007_s10924_023_03081_9 crossref_primary_10_52711_0974_360X_2023_00739 crossref_primary_10_4103_epj_epj_143_22 |
Cites_doi | 10.1186/1754-1611-9-2 10.1007/s00449-017-1864-1 10.1073/pnas.1314063110 10.3390/toxins7020621 10.1073/pnas.87.20.8130 10.1002/anie.200460587 10.1093/dnares/6.2.109 10.1002/mbo3.731 10.1128/jb.176.18.5735-5752.1994 10.1016/bs.ampbs.2020.07.002 10.1007/s10570-014-0521-y 10.7150/jca.11779 10.3389/fpls.2014.00252 10.1186/1471-2229-9-99 |
ContentType | Journal Article |
Copyright | 2022 Elsevier Inc. |
Copyright_xml | – notice: 2022 Elsevier Inc. |
DBID | AAYXX CITATION |
DOI | 10.1016/j.genrep.2022.101577 |
DatabaseName | CrossRef |
DatabaseTitle | CrossRef |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 2452-0144 |
ExternalDocumentID | 10_1016_j_genrep_2022_101577 S2452014422000851 |
GroupedDBID | --M 0R~ 457 4G. 7-5 AACTN AAEDT AAEDW AAIAV AAKOC AALRI AAOAW AAXUO ABMAC ABUDA ABYKQ ACDAQ ACGFS ACRLP ADBBV ADEZE AEBSH AEHWI AFKWA AFTJW AFXIZ AGHFR AGUBO AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ AXJTR BKOJK BLXMC EBS EFJIC EFLBG EJD FDB FIRID FYGXN KOM O9- OAUVE ROL SPCBC SSU SSZ T5K ~G- AAQFI AAXKI AAYXX AFJKZ CITATION |
ID | FETCH-LOGICAL-c306t-b969d179bd7ff2f4614922496403e23c2b39d1b17fc1d3082f1658000a58a553 |
IEDL.DBID | AIKHN |
ISSN | 2452-0144 |
IngestDate | Thu Sep 26 16:01:42 EDT 2024 Fri Feb 23 02:39:48 EST 2024 |
IsPeerReviewed | false |
IsScholarly | true |
Keywords | GT-A BC bcs kb Bacterial cellulose Molecular cloning bp M. wt PCR bcs-operon |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c306t-b969d179bd7ff2f4614922496403e23c2b39d1b17fc1d3082f1658000a58a553 |
ParticipantIDs | crossref_primary_10_1016_j_genrep_2022_101577 elsevier_sciencedirect_doi_10_1016_j_genrep_2022_101577 |
PublicationCentury | 2000 |
PublicationDate | June 2022 2022-06-00 |
PublicationDateYYYYMMDD | 2022-06-01 |
PublicationDate_xml | – month: 06 year: 2022 text: June 2022 |
PublicationDecade | 2020 |
PublicationTitle | Gene reports |
PublicationYear | 2022 |
Publisher | Elsevier Inc |
Publisher_xml | – name: Elsevier Inc |
References | Buldum, Bismarck, Mantalaris (bb0015) 2018; 41 Delmer, Amor (bb0025) 1995; 7 Mehta, Pfeffer, Brown (bb0045) 2015; 22 Matthysse (bb0040) 2014; 5 Sambrook, Russel (bb0060) 2001; Vol.2 Ryngajłło, Kubiak, Jędrzejczak-Krzepkowska, Jacek, Bielecki (bb0055) 2019; 8 Sokollek, Hertel, Hammes (bb0070) 1998; 3 Bielecki, Krystynowicz, Turkiewicz, Kalinowska (bb0005) 2005 Vijayendran, Emmanuel, Ipsita (bb0080) 2020; 77 Yin, Huang, Xu (bb0095) 2009; 9 Saxena, Kudlicka, Okuda, Brown (bb0065) 1994; 176 Klemm, Heublein, Fink, Bohn (bb0035) 2005; 44 Umeda, Hirano, Ishibashi, Akiyama, Onizuka, Ikeuchi, Inoue (bb0075) 1999; 6 Wong, Fear, Calhoon, Eichinger, Mayer, Amikam, Benziman, Gelfand, Meade, Emerick (bb0090) 1990; 87 Bothe, Dutow, Pich, Genth, Klos (bb0010) 2015; 7 Omadjela, Narahari, Strumillo, Mélida, Mazur, Bulone, Zimmer (bb0050) 2013; 110 Hoseini, Sauer (bb0030) 2015; 9 Chawla, Bajaj, Survase, Singhal (bb0020) 2009; 47 Wang, Chen, Zhang, Jiao, Mo, Yang, Hu, Zhang, Wei, Xiang (bb0085) 2015; 6 Saxena (10.1016/j.genrep.2022.101577_bb0065) 1994; 176 Delmer (10.1016/j.genrep.2022.101577_bb0025) 1995; 7 Bielecki (10.1016/j.genrep.2022.101577_bb0005) 2005 Bothe (10.1016/j.genrep.2022.101577_bb0010) 2015; 7 Omadjela (10.1016/j.genrep.2022.101577_bb0050) 2013; 110 Sambrook (10.1016/j.genrep.2022.101577_bb0060) 2001; Vol.2 Klemm (10.1016/j.genrep.2022.101577_bb0035) 2005; 44 Ryngajłło (10.1016/j.genrep.2022.101577_bb0055) 2019; 8 Chawla (10.1016/j.genrep.2022.101577_bb0020) 2009; 47 Matthysse (10.1016/j.genrep.2022.101577_bb0040) 2014; 5 Mehta (10.1016/j.genrep.2022.101577_bb0045) 2015; 22 Buldum (10.1016/j.genrep.2022.101577_bb0015) 2018; 41 Hoseini (10.1016/j.genrep.2022.101577_bb0030) 2015; 9 Sokollek (10.1016/j.genrep.2022.101577_bb0070) 1998; 3 Umeda (10.1016/j.genrep.2022.101577_bb0075) 1999; 6 Wong (10.1016/j.genrep.2022.101577_bb0090) 1990; 87 Vijayendran (10.1016/j.genrep.2022.101577_bb0080) 2020; 77 Wang (10.1016/j.genrep.2022.101577_bb0085) 2015; 6 Yin (10.1016/j.genrep.2022.101577_bb0095) 2009; 9 |
References_xml | – volume: Vol.2 year: 2001 ident: bb0060 article-title: Molecular cloning: a laboratory manual publication-title: Chapter 8: In vitro Amplification of DNA by the Polymerase Chain Reaction contributor: fullname: Russel – volume: 8 year: 2019 ident: bb0055 article-title: Comparative genomics of the komagataeibacter strains-efficient bionanocellulose producers publication-title: MicrobiologyOpen contributor: fullname: Bielecki – volume: 176 start-page: 5735 year: 1994 end-page: 5752 ident: bb0065 article-title: Characterization of genes in the cellulose-synthesizing operon (acs operon) of acetobacter xylinum: implications for cellulose crystallization publication-title: J. Bacteriol. contributor: fullname: Brown – volume: 9 start-page: 99 year: 2009 ident: bb0095 article-title: The cellulose synthase superfamily in fully sequenced plants and algae publication-title: BMC Plant Biol. contributor: fullname: Xu – volume: 22 start-page: 119 year: 2015 end-page: 137 ident: bb0045 article-title: Characterization of an acsD disruption mutant provides additional evidence for the hierarchical cell-directed self-assembly of cellulose in gluconacetobacter xylinus publication-title: Cellulose contributor: fullname: Brown – start-page: 31 year: 2005 end-page: 84 ident: bb0005 publication-title: Bacterial Cellulose. Polysaccharides and Polymers in the Food Industry: Properties, Production, and Patents contributor: fullname: Kalinowska – volume: 7 start-page: 621 year: 2015 end-page: 637 ident: bb0010 article-title: DXD motif-dependent and independent effects of the chlamydia trachomatis cytotoxin CT166 publication-title: Toxins contributor: fullname: Klos – volume: 44 start-page: 3358 year: 2005 end-page: 3393 ident: bb0035 article-title: Cellulose: fascinating biopolymer and sustainable raw material publication-title: Angew. Chem. Int. Ed. contributor: fullname: Bohn – volume: 5 start-page: 252 year: 2014 ident: bb0040 article-title: Attachment of agrobacterium to plant surfaces publication-title: Front. Plant Sci. contributor: fullname: Matthysse – volume: 6 start-page: 632 year: 2015 end-page: 635 ident: bb0085 article-title: Restriction-based multiple-fragment assembly strategy to avoid random mutation during long cDNA cloning publication-title: J. Cancer contributor: fullname: Xiang – volume: 87 start-page: 8130 year: 1990 end-page: 8134 ident: bb0090 article-title: Genetic organization of the cellulose synthase operon in acetobacter xylinum publication-title: Proc. Natl. Acad. Sci. U. S. A. contributor: fullname: Emerick – volume: 6 start-page: 109 year: 1999 end-page: 115 ident: bb0075 article-title: Cloning of cellulose synthase genes from acetobacter xylinum JCM 7664: implication of a novel set of cellulose synthase genes publication-title: DNA Res. contributor: fullname: Inoue – volume: 41 start-page: 265 year: 2018 end-page: 279 ident: bb0015 article-title: Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli publication-title: Bioprocess Biosyst. Eng. contributor: fullname: Mantalaris – volume: 47 start-page: 107 year: 2009 end-page: 124 ident: bb0020 article-title: Microbial cellulose: fermentative production and applications publication-title: Food Technol. Biotechnol. contributor: fullname: Singhal – volume: 110 start-page: 17856 year: 2013 end-page: 17861 ident: bb0050 article-title: BcsA and BcsB form the catalytically active core of bacterial cellulose synthase sufficient for in vitro cellulose synthesis publication-title: Proc. Natl. Acad. Sci. U. S. A. contributor: fullname: Zimmer – volume: 3 start-page: 199 year: 1998 end-page: 206 ident: bb0070 article-title: Cultivation and preservation of vinegar bacteria publication-title: J. Biotech. contributor: fullname: Hammes – volume: 9 start-page: 2 year: 2015 ident: bb0030 article-title: Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering publication-title: J. Biol. Eng. contributor: fullname: Sauer – volume: 77 start-page: 89 year: 2020 end-page: 138 ident: bb0080 article-title: Chapter three - bacterial cellulose: biosynthesis, production, and applications publication-title: Adv. Microb. Physiol. contributor: fullname: Ipsita – volume: 7 start-page: 987 year: 1995 end-page: 1000 ident: bb0025 article-title: Cellulose biosynthesis publication-title: Plant Cell contributor: fullname: Amor – volume: 9 start-page: 2 year: 2015 ident: 10.1016/j.genrep.2022.101577_bb0030 article-title: Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering publication-title: J. Biol. Eng. doi: 10.1186/1754-1611-9-2 contributor: fullname: Hoseini – volume: 41 start-page: 265 issue: 2 year: 2018 ident: 10.1016/j.genrep.2022.101577_bb0015 article-title: Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli publication-title: Bioprocess Biosyst. Eng. doi: 10.1007/s00449-017-1864-1 contributor: fullname: Buldum – volume: 110 start-page: 17856 issue: 44 year: 2013 ident: 10.1016/j.genrep.2022.101577_bb0050 article-title: BcsA and BcsB form the catalytically active core of bacterial cellulose synthase sufficient for in vitro cellulose synthesis publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.1314063110 contributor: fullname: Omadjela – volume: 7 start-page: 621 issue: 2 year: 2015 ident: 10.1016/j.genrep.2022.101577_bb0010 article-title: DXD motif-dependent and independent effects of the chlamydia trachomatis cytotoxin CT166 publication-title: Toxins doi: 10.3390/toxins7020621 contributor: fullname: Bothe – volume: 87 start-page: 8130 issue: 20 year: 1990 ident: 10.1016/j.genrep.2022.101577_bb0090 article-title: Genetic organization of the cellulose synthase operon in acetobacter xylinum publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.87.20.8130 contributor: fullname: Wong – volume: 44 start-page: 3358 year: 2005 ident: 10.1016/j.genrep.2022.101577_bb0035 article-title: Cellulose: fascinating biopolymer and sustainable raw material publication-title: Angew. Chem. Int. Ed. doi: 10.1002/anie.200460587 contributor: fullname: Klemm – volume: 6 start-page: 109 issue: 2 year: 1999 ident: 10.1016/j.genrep.2022.101577_bb0075 article-title: Cloning of cellulose synthase genes from acetobacter xylinum JCM 7664: implication of a novel set of cellulose synthase genes publication-title: DNA Res. doi: 10.1093/dnares/6.2.109 contributor: fullname: Umeda – volume: 8 issue: 5 year: 2019 ident: 10.1016/j.genrep.2022.101577_bb0055 article-title: Comparative genomics of the komagataeibacter strains-efficient bionanocellulose producers publication-title: MicrobiologyOpen doi: 10.1002/mbo3.731 contributor: fullname: Ryngajłło – volume: Vol.2 year: 2001 ident: 10.1016/j.genrep.2022.101577_bb0060 article-title: Molecular cloning: a laboratory manual contributor: fullname: Sambrook – volume: 176 start-page: 5735 issue: 18 year: 1994 ident: 10.1016/j.genrep.2022.101577_bb0065 article-title: Characterization of genes in the cellulose-synthesizing operon (acs operon) of acetobacter xylinum: implications for cellulose crystallization publication-title: J. Bacteriol. doi: 10.1128/jb.176.18.5735-5752.1994 contributor: fullname: Saxena – volume: 77 start-page: 89 year: 2020 ident: 10.1016/j.genrep.2022.101577_bb0080 article-title: Chapter three - bacterial cellulose: biosynthesis, production, and applications publication-title: Adv. Microb. Physiol. doi: 10.1016/bs.ampbs.2020.07.002 contributor: fullname: Vijayendran – volume: 22 start-page: 119 year: 2015 ident: 10.1016/j.genrep.2022.101577_bb0045 article-title: Characterization of an acsD disruption mutant provides additional evidence for the hierarchical cell-directed self-assembly of cellulose in gluconacetobacter xylinus publication-title: Cellulose doi: 10.1007/s10570-014-0521-y contributor: fullname: Mehta – volume: 3 start-page: 199 issue: 3 year: 1998 ident: 10.1016/j.genrep.2022.101577_bb0070 article-title: Cultivation and preservation of vinegar bacteria publication-title: J. Biotech. contributor: fullname: Sokollek – volume: 6 start-page: 632 issue: 7 year: 2015 ident: 10.1016/j.genrep.2022.101577_bb0085 article-title: Restriction-based multiple-fragment assembly strategy to avoid random mutation during long cDNA cloning publication-title: J. Cancer doi: 10.7150/jca.11779 contributor: fullname: Wang – volume: 5 start-page: 252 year: 2014 ident: 10.1016/j.genrep.2022.101577_bb0040 article-title: Attachment of agrobacterium to plant surfaces publication-title: Front. Plant Sci. doi: 10.3389/fpls.2014.00252 contributor: fullname: Matthysse – volume: 9 start-page: 99 year: 2009 ident: 10.1016/j.genrep.2022.101577_bb0095 article-title: The cellulose synthase superfamily in fully sequenced plants and algae publication-title: BMC Plant Biol. doi: 10.1186/1471-2229-9-99 contributor: fullname: Yin – volume: 7 start-page: 987 issue: 7 year: 1995 ident: 10.1016/j.genrep.2022.101577_bb0025 article-title: Cellulose biosynthesis publication-title: Plant Cell contributor: fullname: Delmer – volume: 47 start-page: 107 issue: 2 year: 2009 ident: 10.1016/j.genrep.2022.101577_bb0020 article-title: Microbial cellulose: fermentative production and applications publication-title: Food Technol. Biotechnol. contributor: fullname: Chawla – start-page: 31 year: 2005 ident: 10.1016/j.genrep.2022.101577_bb0005 contributor: fullname: Bielecki |
SSID | ssj0001671974 |
Score | 2.2650115 |
Snippet | This study aimed to assess a novel approach for amplifying and molecular cloning of the four genes encoding bacterial cellulose synthase (bcs-ABCD).
The four... |
SourceID | crossref elsevier |
SourceType | Aggregation Database Publisher |
StartPage | 101577 |
SubjectTerms | Bacterial cellulose bcs-operon Molecular cloning PCR |
Title | Novel approach of amplification and cloning of bacterial cellulose synthesis (bcs) operon from Gluconoacetobacter hansenii |
URI | https://dx.doi.org/10.1016/j.genrep.2022.101577 |
Volume | 27 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NS8MwFA9zQ_AifuL8IgcPeihr0zRpj2OoU3EXJ-xWmjbBymiH3YT51_te06KCePDYhBfKS99HXn_vF0IufNd4KgP7zpRrHM7SzFHSRdif9GXEFAsVNic_TsT4md_PglmHjNpeGIRVNr7f-vTaWzcjg0abg0WeD57wnyGeBxizicMG6UE44rxLesO7h_Hkq9QipBfVfMwo4qBM20RXI73qnULuSsZwKJDy9yD1LfDc7JDtJmOkQ_tSu6Sjiz2yae-QXO-Tj0n5rue05QanpaEJosRNU4yjSZHRdF5XXXFSWXpmWBFr9qt5WWlarQvIA6u8opcqra5oiezhBcXWE3qLmPYCltZo-ihLXxIsSOf5AZneXE9HY6e5UMFJ4WSwdFQkogwsUGXSGGY4hOYIQngkuOtr5qdM-TCvPGlSL0MeG-NBggIaTIIwCQL_kHSLstBHhAqmuBFhKlI4ISaBChOhOdMexEPDhNF94rQKjBeWNiNu8WSvsVV4jAqPrcL7RLZajn9sfwye_U_J439LnpAtfLK4r1PSXb6t9BlkGEt13nxBn-87z4w |
link.rule.ids | 315,786,790,24144,27957,27958,45714 |
linkProvider | Elsevier |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV07T8MwED6VVggWxFOUpwcGGKI2TmI3Y1VRWvpYKFK3KE5sEVQlFWmRyq_HFycCJMTAGuus6HN8d7589xngxmkrW8R6f8eirSyXRrEleBtpf9zhPhW0I7A5eTJlg2f3ce7Na9CremGQVln6fuPTC29dPmmVaLaWSdJ6wn-GeB6g1CQOW9BwPW67dWh0h6PB9KvUwrjtF3rMaGKhTdVEVzC9ipVC7UpK8ZHH-e9B6lvg6e_DXpkxkq55qQOoyfQQts0dkpsj-Jhm73JBKm1wkikSIktclcU4EqYxiRZF1RUHhZFn1jNizX69yHJJ8k2q88A8ycmtiPI7kqF6eEqw9YQ8IKc91VNL3PpoS15CLEgnyTHM-vez3sAqL1SwIn0yWFnCZ36sd6CIuVJUuTo0-zqE-8xtO5I6ERWOHhc2V5Edo46NsnWCohEMvU7oec4J1NMsladAGBWuYp2IRfqEGHqiEzLpUmnreKgoU7IJVgVgsDSyGUHFJ3sNDOABAh4YwJvAK5SDH8sfaM_-p-XZvy2vYWcwm4yD8XA6OoddHDEcsAuor97W8lJnGytxVX5Nn9h90nw |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Novel+approach+of+amplification+and+cloning+of+bacterial+cellulose+synthesis+%28bcs%29+operon+from+Gluconoacetobacter+hansenii&rft.jtitle=Gene+reports&rft.au=Al-Janabi%2C+Saif+S.&rft.au=Shawky%2C+Heba&rft.au=El-Waseif%2C+Amr+A.&rft.au=Farrag%2C+Ayman+A.&rft.date=2022-06-01&rft.pub=Elsevier+Inc&rft.issn=2452-0144&rft.eissn=2452-0144&rft.volume=27&rft_id=info:doi/10.1016%2Fj.genrep.2022.101577&rft.externalDocID=S2452014422000851 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2452-0144&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2452-0144&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2452-0144&client=summon |