Direct fermentation of cellulose to ethanol by Saccharomyces cerevisiae displaying a bifunctional cellobiohydrolase gene from Orpinomyces sp. Y102

• Published in: Renewable energy Vol. 159; pp. 1029 - 1035
• Main Authors: Liu, Jeng-Chen, Chang, Wan-Jhu, Hsu, Teng-Chieh, Chen, Hui-Jye, Chen, Yo-Chia
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2020
• Subjects: Anaerobic rumen fungus; Bioethanol; Cellobiohydrolase; Orpinomyces; β-glucosidase; Bioethanol; Orpinomyces; β-glucosidase; Anaerobic rumen fungus; Cellobiohydrolase

A cellobiohydrolase gene, cbhC16, isolated from the cDNA library of a rumen fungus. The recombinant CbhC16 demonstrated a specific activity of 7.0 U mg−1 protein against phosphoric acid-swollen avicel, and cellobiose was the main product in the reaction mixture. Glucose was further released from cel...