A cell-free system of Drosophila egg explants supporting native mitotic cycles

• Published in: Methods in cell biology Vol. 144; pp. 233 - 257
• Main Authors: de-Carvalho, Jorge, Deshpande, Ojas, Nabais, Catarina, Telley, Ivo A
• Format: Book Chapter Journal Article
• Language: English
• Published: United States Elsevier Science & Technology 2018
• Subjects: Animals; Cell Extracts; Cell-Free System; Cytological Techniques - methods; Drosophila melanogaster - cytology; Drosophila melanogaster - genetics; Female; Image Processing, Computer-Assisted; Microscopy; Mitosis; Ovum - cytology; Time Factors; Mitotic cycling; Cell-free; Extract; Ex vivo; Nuclear division; Drosophila

Mitosis, in a broader sense, is an intracellular mechanical process that is fueled by chemical reactions and regulated by a complex protein interaction network. Research aimed at understanding mitosis in all these aspects is often limited to pharmaceutical treatment or genetic manipulation of single...