A novel all-in-one target-powered entropy-driven dynamic DNA networks to regulate the activity of CRISPR/AsCas12a for enhanced DNA detection

• Published in: Analytica chimica acta Vol. 1335; p. 343455
• Main Authors: Li, Xingrong, Wang, Cuixiang, Chai, Jiatong, Liu, Hongmao, Jiang, Xinli, Li, Yumei, Li, Zhiqiang, Li, Yirong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.01.2025
• Subjects: Activator strand; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biosensing; Biosensing Techniques - methods; CRISPR-Associated Proteins - chemistry; CRISPR-Associated Proteins - metabolism; CRISPR-Cas Systems - genetics; CRISPR/AsCas12a; DNA; DNA - chemistry; Endodeoxyribonucleases - chemistry; Endodeoxyribonucleases - metabolism; Entropy; Entropy driven dynamic DNA network; Nucleic Acid Amplification Techniques; Nucleic Acid Hybridization; Strand displacement reaction; Entropy driven dynamic DNA network; Activator strand; Strand displacement reaction; CRISPR/AsCas12a; DNA; Biosensing

The non-enzyme autonomous DNA nanodevices have been developed to detect various analytes through the programmability of Watson-Crick base pairing. Nevertheless, by comparison with enzymatic biosensors, the usage of enzyme-free DNA networks to create biosensors for testing low amounts of targets is s...