Insect cells of Spodoptera frugiperda support WSSV gene replication but not progeny virion assembly

• Published in: Developmental and comparative immunology Vol. 156; p. 105160
• Main Authors: Jing, Shan-Shan, Liu, Ling-Ke, Liu, Hai-Peng
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.07.2024
• Subjects: Animals; Cell Line; Cell Nucleus - metabolism; Cell Nucleus - virology; DNA Virus Infections - immunology; DNA Virus Infections - virology; Genome, Viral; Nucleocapsid - genetics; Nucleocapsid - metabolism; Progeny assemble; Sf9 cell line; Sf9 Cells; Spodoptera - virology; Viral Envelope Proteins - genetics; Viral Envelope Proteins - metabolism; Viral gene transcription; Viral protein expression; Virion - metabolism; Virus Assembly; Virus Replication; White spot syndrome virus; White spot syndrome virus 1 - physiology; Viral protein expression; Viral gene transcription; White spot syndrome virus; Sf9 cell line; Progeny assemble
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2024.105160

The lacking of stable and susceptible cell lines has hampered research on pathogenic mechanism of crustacean white spot syndrome virus (WSSV). To look for the suitable cell line which can sustain WSSV infection, we performed the studies on WSSV infection in the Spodoptera frugiperda (Sf9) insect cells. In consistent with our previous study in vitro in crayfish hematopoietic tissue cells, the WSSV envelope was detached from nucleocapsid around 2 hpi in Sf9 cells, which was accompanied with the cytoplasmic transport of nucleocapsid toward the cell nucleus within 3 hpi. Furthermore, the expression profile of both gene and protein of WSSV was determined in Sf9 cells after viral infection, in which a viral immediate early gene IE1 and an envelope protein VP28 exhibited gradually increased presence from 3 to 24 hpi. Similarly, the significant increase of WSSV genome replication was found at 3–48 hpi in Sf9 cells after infection with WSSV, indicating that Sf9 cells supported WSSV genome replication. Unfortunately, no assembled progeny virion was observed at 24 and 48 hpi in Sf9 cell nuclei as determined by transmission electron microscope, suggesting that WSSV progeny could not be assembled in Sf9 cell line as the viral structural proteins could not be transported into cell nuclei. Collectively, these findings provide a cell model for comparative analysis of WSSV infection mechanism with crustacean cells. •WSSV completes intracellular trafficking in Sf9 cells.•Both gene replication and proteins synthesis of WSSV occur in Sf9 cell.•No WSSV progeny virion assembles in Sf9 cell nuclei.