Detailed protocol for optimised expression and purification of functional monomeric human Heat Shock Factor 1

• Published in: Protein expression and purification Vol. 176; p. 105722
• Main Authors: Polidano, Joseph, Vankadari, Naveen, Price, John T., Wilce, Jacqueline A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2020
• Subjects: Gene Expression; Heat shock factor; Heat Shock Transcription Factors - biosynthesis; Heat Shock Transcription Factors - chemistry; Heat Shock Transcription Factors - genetics; Heat Shock Transcription Factors - isolation & purification; Humans; Oligomerisation; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; Stress response; Transcription factor; Heat shock factor; Transcription factor; Stress response; Oligomerisation

Heat Shock Factor 1 (HSF1) is the master regulator of the heat shock response, a universal survival mechanism throughout eukaryotic species used to buffer potentially lethal proteotoxic conditions. HSF1's function in vivo is regulated by several factors, including post translational modificatio...