Fluorescent nanoparticles using hyaluronic acid-histidine-based manganese dioxide for hydrogen peroxide imaging in inflammatory macrophages

• Published in: Macromolecular research Vol. 33; no. 3; pp. 271 - 276
• Main Authors: Hong, Ji Yeon, Park, Ji Sun, Park, Kyeongsoon
• Format: Journal Article
• Language: English
• Published: Seoul The Polymer Society of Korea 01.03.2025; Springer Nature B.V; 한국고분자학회
• Subjects: Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Communication; Complex Fluids and Microfluidics; Fluorescence; Histidine; Hyaluronic acid; Hydrogen peroxide; Imaging; Macrophages; Manganese dioxide; Nanochemistry; Nanoparticles; Nanotechnology; Physical Chemistry; Polymer Sciences; Soft and Granular Matter; 고분자공학; Fluorescent nanoparticles; Inflammatory macrophages; ); Hyaluronic acid–histidine conjugates; Manganese dioxide (MnO; Hydrogen peroxide (H; O
• ISSN: 1598-5032; 2092-7673
• DOI: 10.1007/s13233-024-00337-8

The presence of excessive hydrogen peroxide (H 2 O 2 ) in the body plays a key role in inflammatory diseases. Among the various methods available, fluorescent nanoparticles have been used to detect H 2 O 2 in living cells. Herein, the design of CD44-targetable fluorescent nanoparticles is presented for intracellular H 2 O 2 imaging in inflammatory macrophages. Accordingly, CD44-targetable hyaluronic acid-histidine (HA–His) conjugates were sequentially reacted with permanganate and fluorescein (FL) to synthesize HA–His/MnO 2 /FL nanoparticles. These prepared HA–His/MnO 2 /FL nanoparticles exhibited good dispersibility in water and 10% fetal bovine serum solution. Owing to their rapid responsiveness to H 2 O 2 , the HA–His/MnO 2 /FL nanoparticles showed a good linear relationship with H 2 O 2 concentration (0–100 μM). Moreover, the CD44-targetable HA–His/MnO 2 /FL nanoparticles were readily internalized in lipopolysaccharide-induced inflammatory macrophages, allowing successful imaging of intracellular H 2 O 2 in inflammatory macrophages. Graphical Abstract The HA-His/MnO 2 /FL nanoparticles with H 2 O 2 responsiveness were readily internalized into activated macrophages, allowing successful intracellular H 2 O 2 imaging in inflammatory macrophages