Identification and genomic characterization of a novel porcine CRESS DNA virus from a pig suffering from diarrhea in China

• Published in: Archives of virology Vol. 167; no. 5; pp. 1355 - 1359
• Main Authors: Liu, Tian-Ning, Liu, Cong-Xi, Liao, Jing-Ying, Xiong, Wei-Jie, Xia, Jun-Yong, Xiao, Chao-Ting
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.05.2022; Springer Nature B.V
• Subjects: Amino acids; Amino Acids - genetics; Animals; Annotated Sequence Record; Biomedical and Life Sciences; Biomedicine; Brassicaceae - genetics; Capsid protein; Deoxyribonucleic acid; Diarrhea; Diarrhea - veterinary; DNA; DNA viruses; DNA Viruses - genetics; DNA, Viral - genetics; Genome, Viral; Genomes; Genomics; Infectious Diseases; Medical Microbiology; Nucleotide sequence; Nucleotides; Open Reading Frames; Phylogeny; Proteins; Rep protein; Swine; Virology; Viruses; Viruses - genetics
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-022-05423-9

The circular replication-associated protein (Rep)-encoding ssDNA (CRESS-DNA) viruses show high diversity and have a very wide range of hosts, including all three domains of cellular life. In the present study, a novel CRESS DNA virus, provisionally named "kirkovirus HNU-XX-2020" was discovered in a growing pig with watery diarrhea. The virus has a circular genome of 2961 nucleotides (nt) and three major putative open reading frames (ORFs), encoding a Rep protein (327 amino acids), a capsid protein (175 amino acids), and one protein (209 amino acids) of unknown function. The genome showed the highest sequence similarity (68.6% identity) to the genome of porcine circo-like virus 51 (JF713719), which was identified in pig faeces, and it showed very limited sequence similarity (less than 40% identity) to other virus genomes. Further phylogenetic analysis suggested that it could be a novel member in the proposed family " Kirkoviridae " .