Antivirulence and antipathogenic activity of Mayan herbal remedies against Pseudomonas aeruginosa

• Published in: Journal of ethnopharmacology Vol. 332; p. 118373
• Main Authors: Espíndola-Rodríguez, Nadine Heidi, Muñoz-Cázares, Naybi, Serralta-Peraza, Lidia Esther del Socorro, Díaz-Nuñez, José Luis, Montoya-Reyes, Francisco, García-Contreras, Rodolfo, Díaz-Guerrero, Miguel, Rivera-Chávez, José Alberto, Gutiérrez, Jorge, Sotelo-Barrera, Mireya, Castillo-Juárez, Israel
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 05.10.2024
• Subjects: Ancientbiotics; Antivirulence; Burns; Mayan herbal remedies; Pseudomonas aeruginosa; Antivirulence; Pseudomonas aeruginosa; Burns; Ancientbiotics; Mayan herbal remedies
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2024.118373

The Yucatan Peninsula has a privileged wealth of vascular plants with which various Mayan herbal formulations have been developed. However, studies on their antipathogenic and antivirulence properties are scarce. Identify antivirulence properties in Mayan herbal remedies and determine their antipathogenic capacity in burn wounds infected with Pseudomonas aeruginosa. An ethnobotanical study was conducted in Mayan communities in central and southern Quintana Roo, Mexico. Furthermore, the antipathogenic capacity of three Mayan herbal remedies was analyzed using an animal model of thermal damage and P. aeruginosa infection. Antivirulence properties were determined by inhibiting phenotypes regulated by quorum sensing (pyocyanin, biofilm, and swarming) and by the secretion of the ExoU toxin. The chemical composition of the most active herbal remedy was analyzed using molecular network analysis. It was found that topical administration of the remedy called “herbal soap” (HS) for eleven days maintained 100% survival of the animals, reduced establishment of the bacteria in the burn and prevented its systemic dispersion. Although no curative effect was recorded on tissue damaged by HS treatment, its herbal composition strongly reduced swarming and ExoU secretion. Through analysis of Molecular Networks, it was possible to carry out a global study of its chemical components, and identify the family of oxindole monoterpenoid alkaloids and carboline and tetrahydropyrididole alkaloids. In addition, flavonols, flavan-3-ols, and quinic acid derivatives were detected. The antipathogenic and antivirulence capacity of ancient Mayan remedies makes them a potential resource for developing new antibacterial therapies to treat burns infected by P. aeruginosa. [Display omitted] •Multidisciplinary research on ancient herbal remedies is a strategy for designing treatments against current infections.•Mayan herb formulations have antibacterial properties with antivirulence-type mechanisms of action.•The Mayan “herbal soap” formulation exhibits antipathogenic capacity and effectively treats burns infected with P. aeruginosa.