Development and evaluation of orbital rocking motion-based single-use cell culture system, the CELBIC® system

The importance of a single-use bioreactor (SUB) is continuously increasing in research and industrial fields for biopharmaceutical production. In this study, a newly developed SUB system, the CELBIC ® system, applied with the unique agitation method is introduced, and its physical properties and bio...

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Published inBiotechnology and bioprocess engineering Vol. 29; no. 3; pp. 513 - 519
Main Authors Kim, Hyunwoo, Park, Seohyun, Kim, Rock Ki, Baik, Yeong Ok, Kim, Kyung Nam, Oh, Duk Jae
Format Journal Article
LanguageEnglish
Published Seoul The Korean Society for Biotechnology and Bioengineering 01.06.2024
Springer Nature B.V
한국생물공학회
Subjects
Aeration
Biological properties
Bioreactors
Biotechnology
Cell culture
Cell lines
Chemistry
Chemistry and Materials Science
Industrial and Production Engineering
Mass transfer
Medical research
Pharmaceuticals
Physical properties
Research Paper
생물공학
system
Mammalian cell culture
Single-use bioreactor
Mixing time
CELBIC
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Abstract The importance of a single-use bioreactor (SUB) is continuously increasing in research and industrial fields for biopharmaceutical production. In this study, a newly developed SUB system, the CELBIC ® system, applied with the unique agitation method is introduced, and its physical properties and biological applications are evaluated. The mixing time was in the range of 11.0–129.0 s, and the volumetric mass transfer coefficient, k L a , measured through surface aeration, was in the range of 2.7–15.5 h −1 at a working volume of 1–10 L. Biological evaluations using two Chinese hamster ovary (CHO) cell lines, CHO-DG44 and CHO-S cell, were carried out in the CELBIC ® systems in 1 and 50 L scales, showing similar cell culture performance as that from stirred tank-type bioreactors. These results support the CELBIC ® system as a new SUB system applicable to cell cultures for biopharmaceutical production.
AbstractList The importance of a single-use bioreactor (SUB) is continuously increasing in research and industrial fields for biopharmaceutical production. In this study, a newly developed SUB system, the CELBIC® system, applied with the unique agitation method is introduced, and its physical properties and biological applications are evaluated. The mixing time was in the range of 11.0–129.0 s, and the volumetric mass transfer coefficient, kLa, measured through surface aeration, was in the range of 2.7–15.5 h−1 at a working volume of 1–10 L. Biological evaluations using two Chinese hamster ovary (CHO) cell lines, CHO-DG44 and CHO-S cell, were carried out in the CELBIC® systems in 1 and 50 L scales, showing similar cell culture performance as that from stirred tank-type bioreactors. These results support the CELBIC® system as a new SUB system applicable to cell cultures for biopharmaceutical production.
The importance of a single-use bioreactor (SUB) is continuously increasing in research and industrial fields for biopharmaceutical production. In this study, a newly developed SUB system, the CELBIC ® system, applied with the unique agitation method is introduced, and its physical properties and biological applications are evaluated. The mixing time was in the range of 11.0–129.0 s, and the volumetric mass transfer coefficient, k L a , measured through surface aeration, was in the range of 2.7–15.5 h −1 at a working volume of 1–10 L. Biological evaluations using two Chinese hamster ovary (CHO) cell lines, CHO-DG44 and CHO-S cell, were carried out in the CELBIC ® systems in 1 and 50 L scales, showing similar cell culture performance as that from stirred tank-type bioreactors. These results support the CELBIC ® system as a new SUB system applicable to cell cultures for biopharmaceutical production.
The importance of a single-use bioreactor (SUB) is continuously increasing in research and industrial fields for biopharmaceutical production. In this study, a newly developed SUB system, the CELBIC ® system, applied with the unique agitation method is introduced, and its physical properties and biological applications are evaluated. The mixing time was in the range of 11.0–129.0 s, and the volumetric mass transfer coeffi cient, k L a , measured through surface aeration, was in the range of 2.7–15.5 h −1 at a working volume of 1–10 L. Biological evaluations using two Chinese hamster ovary (CHO) cell lines, CHO-DG44 and CHO-S cell, were carried out in the CELBIC ® systems in 1 and 50 L scales, showing similar cell culture performance as that from stirred tank-type bioreactors. These results support the CELBIC ® system as a new SUB system applicable to cell cultures for biopharmaceutical production. KCI Citation Count: 0
Author Park, Seohyun
Kim, Kyung Nam
Kim, Hyunwoo
Baik, Yeong Ok
Kim, Rock Ki
Oh, Duk Jae
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  surname: Kim
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  surname: Kim
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  givenname: Yeong Ok
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  givenname: Kyung Nam
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  surname: Oh
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  organization: Department of Integrative Bioscience and Biotechnology, Sejong University
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10.1002/bit.27000
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10.1016/j.tibtech.2012.10.004
10.1016/j.bej.2005.08.009
10.1002/cite.201200149
10.1007/10_2008_15
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Mammalian cell culture
Single-use bioreactor
Mixing time
CELBIC
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SubjectTerms Aeration
Biological properties
Bioreactors
Biotechnology
Cell culture
Cell lines
Chemistry
Chemistry and Materials Science
Industrial and Production Engineering
Mass transfer
Medical research
Pharmaceuticals
Physical properties
Research Paper
생물공학
Title Development and evaluation of orbital rocking motion-based single-use cell culture system, the CELBIC® system
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