Association of the apolipoprotein E ε4 allele in a Serbian population with Alzheimer’s dementia

• Published in: Archives of biological sciences Vol. 75; no. 3; pp. 319 - 325
• Main Authors: Basic, Jelena, Milosevic, Vuk, Djordjevic, Branka, Stefanovic, Nikola, Cvetkovic, Marija, Jevtovic-Stoimenov, Tatjana, Stojanovic, Ivana
• Format: Journal Article
• Language: English
• Published: University of Belgrade, University of Novi Sad 2023
• Subjects: alzheimer’s disease; apolipoprotein e ε4; dementia; rs429358; rs7412
• ISSN: 0354-4664; 1821-4339
• DOI: 10.2298/ABS230614026B

Although single nucleotide polymorphisms rs429358 and rs7412 in the apolipoprotein E gene (APOE) are widely investigated to analyze APOE alleles, there are no data on their association with Alzheimer?s disease (AD) dementia in a Serbian population. This study aimed to investigate the distribution of APOE ?2/?3/?4 alleles in patients with dementia due to AD and cognitively unimpaired subjects and to assess the association of the APOE ?4 allele with disease risk in the Serbian population. A case-control study included patients with dementia due to AD and cognitively unimpaired individuals. APOE rs429358 and rs7412 were analyzed using the Real-Time PCR method with allele-specific TaqMan assays, followed by APOE ?2/?3/?4 allele carrier status analysis. Patients had a significantly higher frequency of the APOE ?4 allele than the control group (P<0.001). The APOE ?4 allele was found to be associated with a 3-fold higher risk of AD dementia compared to the reference ?3 allele (P<0.001). In conclusion, this is the first study to suggest that carriers of the APOE ?4 allele have a higher risk of developing dementia due to AD than those who carry the APOE ?3 allele in the Serbian population.