Visualization, Inspection and Interpretation of Shotgun Proteomics Identification Results

Shotgun proteomics is a high throughput technique for protein identification able to identify up to several thousand proteins from a single sample. In order to make sense of this large amount of data, proteomics analysis software is needed, aimed at making the data intuitively accessible to beginner...

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Bibliographic Details
Published inAdvances in experimental medicine and biology Vol. 919; p. 227
Main Authors Lereim, Ragnhild R, Oveland, Eystein, Berven, Frode S, Vaudel, Marc, Barsnes, Harald
Format Journal Article
LanguageEnglish
Published United States 2016
Subjects
Algorithms
Animals
Computational Biology - methods
Data Mining - methods
Databases, Protein
High-Throughput Screening Assays
Humans
Proteins - analysis
Proteome
Proteomics - methods
Reproducibility of Results
Search Engine
Software
User-Computer Interface
Workflow
Validation
Protein identification
Visualization
Protein annotation
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Summary:Shotgun proteomics is a high throughput technique for protein identification able to identify up to several thousand proteins from a single sample. In order to make sense of this large amount of data, proteomics analysis software is needed, aimed at making the data intuitively accessible to beginners as well as experienced scientists. This chapter provides insight on where to start when analyzing shotgun proteomics data, with a focus on explaining the most common pitfalls in protein identification analysis and how to avoid them. Finally, the move to seeing beyond the list of identified proteins and to putting the results into a bigger biological context is discussed.
ISSN:0065-2598
DOI:10.1007/978-3-319-41448-5_11