Over 10 000 Peptide Identifications from the HeLa Proteome by Using Single-Shot Capillary Zone Electrophoresis Combined with Tandem Mass Spectrometry

• Published in: Angewandte Chemie (International ed.) Vol. 53; no. 50; pp. 13931 - 13933
• Main Authors: Sun, Liangliang, Hebert, Alexander S., Yan, Xiaojing, Zhao, Yimeng, Westphall, Michael S., Rush, Matthew J. P., Zhu, Guijie, Champion, Matthew M., Coon, Joshua J., Dovichi, Norman J.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 08.12.2014; WILEY‐VCH Verlag
• Subjects: analytical methods; CZE-MS/MS; electrophoresis; Electrophoresis, Capillary - methods; HeLa Cells; Humans; mass spectrometry; Peptides - chemistry; Proteome; proteomics; Tandem Mass Spectrometry - methods; mass spectrometry; proteomics; electrophoresis; analytical methods; CZE-MS/MS

Capillary zone electrophoresis (CZE)–tandem mass spectrometry (MS/MS) has recently attracted attention as a tool for shotgun proteomics. However, its performance for this analysis has so far fallen far below that of reversed‐phase liquid chromatography (RPLC)–MS/MS. The use of a CZE method with a wi...