Determination of cholecalciferol and related substances by calcium phosphate hydroxyapatite and calcium phosphate fluoroapatite high-performance liquid chromatography

• Published in: Journal of Chromatography A Vol. 731; no. 1-2; pp. 153 - 160
• Main Authors: Benmoussa, A., Delaurent, C., Lacout, J.-L., Loiseau, P.R., Mikou, M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 19.04.1996
• Subjects: Maleic anhydride; Provitamin D3; Stationary phases; Tachysterol; Vitamin D3; Provitamin D3; Vitamin D3; Tachysterol; Stationary phases; LC; Maleic anhydride

A qualitative and comparative study by HPLC using two new stationary phases, hydroxy- and fluoroapatites, showed the separation under optimized conditions of vitamin D3 and its related compounds, produced by irradiation and heating of provitamin D3.