Cloned pigs derived from somatic cell nuclear transfer embryos cultured in vitro at low oxygen tension

• Published in: Chinese science bulletin Vol. 51; no. 7; pp. 839 - 844
• Main Authors: Pan, Dengke, Zhang, Yunhai, Sun, Xiuzhu, Zhang, Jian, Li, Xuyang, Li, Yan, Gu, Zhiliang, Dai, Yunping, Wu, Changxin, Li, Ning
• Format: Journal Article
• Language: English
• Published: College of Animal Science and Technology, China Agricultural University, Beijing 100094, China%State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China%Animal Clinical Hospital, China Agricultural University, Beijing 100094, China%Hebei Minghui Group of Pigs Breeding, Sanhe 065200, China%College of Animal Science and Technology, China Agricultural University, Beijing 100094, China 01.04.2006; State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China
• Subjects: 体外发育; 体细胞克隆; 卵母细胞; 无性繁殖; 迁移晶胚; in vitro maturation of oocyte; low oxygen; nuclear transfer; embryo culture; pig

Pig cloning has great potential to human xenotransplantation. The present study was designed to establish a more efficient system for producing cloned pigs by somatic cell nuclear transfer （SCNT）. Our approach was as follows： SCNT embryos were reconstructed by using fetal fibroblasts of Chinese mini...