Inducing high rates of targeted mutagenesis in zebrafish using zinc finger nucleases (ZFNs)

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 770; p. 505
• Main Authors: McCammon, Jasmine M, Doyon, Yannick, Amacher, Sharon L
• Format: Journal Article
• Language: English
• Published: United States 01.01.2011
• Subjects: Animal Husbandry; Animals; Deoxyribonucleases - chemistry; Deoxyribonucleases - metabolism; Embryo, Nonmammalian - metabolism; Female; Genes, Reporter - genetics; Genetic Loci - genetics; Genetic Techniques; Genotype; Germ Cells - cytology; Germ Cells - metabolism; Male; Microinjections; Mutagenesis; Phenotype; RNA - biosynthesis; Saccharomyces cerevisiae - genetics; Zebrafish - embryology; Zebrafish - genetics; Zinc Fingers

Animal models, including the zebrafish, without a reliable embryonic stem cell system are not easily amenable to targeted mutagenesis for studying gene function. Three recent publications have shown that zinc finger nucleases (ZFNs) have circumvented this shortcoming in zebrafish. Similar to restric...