Fluoride determination by its inhibitory effect on immobilised liver esterase
The inhibition of liver esterase (EC 3.1.1.1.) immobilised on controlled pore glass by fluoride ions was studied in a flow injection system. Ethyl butyrate was used as substrate, ethanol and butyric acid being produced as hydrolysis products. To monitor the reaction a second enzymatic reaction was e...
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| Published in | Analytica chimica acta Vol. 310; no. 1; pp. 173 - 180 |
|---|---|
| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier B.V
25.06.1995
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| Subjects | |
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| Abstract | The inhibition of liver esterase (EC 3.1.1.1.) immobilised on controlled pore glass by fluoride ions was studied in a flow injection system. Ethyl butyrate was used as substrate, ethanol and butyric acid being produced as hydrolysis products. To monitor the reaction a second enzymatic reaction was employed, in which alcohol dehydrogenase, immobilised on controlled pore glass, catalysed the oxidation of ethanol and reduction of the coenzyme, NAD
+, to NADH, the latter being determined spectrophotometrically at 340 nm. Fluoride inhibition of the immobilised liver esterase was fast and reversible, and there was a linear relationship between %inhibition and fluoride concentration over the range 8 × 10
−7−8 × 10
−6
M (15–150
μg 1
−1) fluoride. The relative standard deviation at mid-calibration range is below 4% (
n = 5). The more common inorganic compounds present in water samples do not interfere in the fluoride determination. |
|---|---|
| AbstractList | The inhibition of liver esterase (EC 3.1.1.1.) immobilised on controlled pore glass by fluoride ions was studied in a flow injection system. Ethyl butyrate was used as substrate, ethanol and butyric acid being produced as hydrolysis products. To monitor the reaction a second enzymatic reaction was employed, in which alcohol dehydrogenase, immobilised on controlled pore glass, catalysed the oxidation of ethanol and reduction of the coenzyme, NAD
+, to NADH, the latter being determined spectrophotometrically at 340 nm. Fluoride inhibition of the immobilised liver esterase was fast and reversible, and there was a linear relationship between %inhibition and fluoride concentration over the range 8 × 10
−7−8 × 10
−6
M (15–150
μg 1
−1) fluoride. The relative standard deviation at mid-calibration range is below 4% (
n = 5). The more common inorganic compounds present in water samples do not interfere in the fluoride determination. |
| Author | Marcos, J. Townshend, A. |
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| Cites_doi | 10.1016/0005-2744(81)90087-5 10.1021/ac60156a067 10.1016/0956-5663(90)80035-C 10.1021/ac00273a032 10.1016/S0021-9258(17)45955-2 10.1021/ac50037a030 10.1016/S0003-2670(00)80438-9 10.1016/S0003-2670(01)80043-X 10.1016/0003-2670(93)85254-H 10.1042/bj0570692 10.1016/S0021-9258(18)70394-3 10.1016/0003-2697(71)90159-X 10.1016/0003-2670(94)80234-3 10.1021/ac60218a037 10.1016/S0021-9673(01)95359-2 10.1016/0003-2670(93)80215-7 10.1021/bi00833a037 10.1021/ac50052a046 |
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| Keywords | Fluoride Inhibition Liver esterase Ethyl butyrate Flow injection |
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| Title | Fluoride determination by its inhibitory effect on immobilised liver esterase |
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