Exploring Green Fluorescent Protein Brownian Motion: Temperature and Concentration Dependencies Through Luminescence Thermometry

• Published in: Advanced Physics Research Vol. 3; no. 11
• Main Authors: Guo, Yongwei, Maturi, Fernando E., Brites, Carlos D. S., Carlos, Luís D.
• Format: Journal Article
• Language: English
• Published: Edinburgh John Wiley & Sons, Inc 01.11.2024; Wiley-VCH
• Subjects: ballistic transport; Brownian motion; brownian velocity; Enzyme kinetics; Fluorescence; green fluorescent protein; luminescence thermometry; Nanoparticles; Physiology; Proteins; Spectrum analysis; Star & galaxy formation; Temperature; Temperature dependence; Velocity; Viscosity

Luminescent nanothermometry emerges as a powerful tool for studying protein dynamics. This technique was employed to perform the first measurement of the temperature dependence of protein Brownian velocity, showcasing the illustrative example of enhanced green fluorescent protein (EGFP) across physi...