Biochemical and molecular study of long non-coding RNAs (HOTTIP, ZEB-AS1 and MEG-3) in hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is a main cause of cancer death worldwide. The high mortality of HCC patients is linked to delayed diagnosis. Long non-coding RNAs (lncRNAs) have been demonstrated as significant controllers of various human cancers. This study aimed to clarify the potential diagnostic...

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Published inMeta Gene Vol. 26; p. 100808
Main Authors Soliman, Shimaa E., ElTorgoman, Abdel Monem A., Assar, Mohamed F.A., El Abd, Naglaa S., Gohar, Suzy F., Girgis, Raouth E.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.12.2020
Subjects
Hepatocellular carcinoma
lncRNAs (HOTTIP, ZEB-AS1, MEG3)
Real-time PCR
Real-time PCR
Hepatocellular carcinoma
lncRNAs (HOTTIP, ZEB-AS1, MEG3)
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Abstract Hepatocellular carcinoma (HCC) is a main cause of cancer death worldwide. The high mortality of HCC patients is linked to delayed diagnosis. Long non-coding RNAs (lncRNAs) have been demonstrated as significant controllers of various human cancers. This study aimed to clarify the potential diagnostic and prognostic value of lncRNAs (HOTTIP, ZEB-AS1 and MEG3) in HCC. This study included 150 subjects (50 HCC patients, 50 cirrhotic patients and 50 healthy subjects). lncRNA gene expression was estimated by real-time PCR. HOTTIP and ZEB-AS1 levels were higher in HCC patients than in cirrhotic patients and healthy individuals (P < 0.001). The level of MEG-3 was significantly lower in the HCC group than in the cirrhotic and control groups (P < 0.001). In HCC patients, higher HOTTIP and ZEB-AS1 were linked with nodal and distant metastases, and lower MEG-3 was correlated with larger tumour size; additionally, higher HOTTIP and lower MEG-3 were associated with lower overall survival. ROC curve analysis revealed cut-off values >10.35 > 15 and < 2.3 for HOTTIP, ZEB-AS1 and MEG-3, respectively. HOTTIP, ZEB-AS1 and MEG-3 could be considered diagnostic biomarkers for HCC, and HOTTIP and MEG-3 may represent prognostic biomarkers for HCC.
AbstractList Hepatocellular carcinoma (HCC) is a main cause of cancer death worldwide. The high mortality of HCC patients is linked to delayed diagnosis. Long non-coding RNAs (lncRNAs) have been demonstrated as significant controllers of various human cancers. This study aimed to clarify the potential diagnostic and prognostic value of lncRNAs (HOTTIP, ZEB-AS1 and MEG3) in HCC. This study included 150 subjects (50 HCC patients, 50 cirrhotic patients and 50 healthy subjects). lncRNA gene expression was estimated by real-time PCR. HOTTIP and ZEB-AS1 levels were higher in HCC patients than in cirrhotic patients and healthy individuals (P < 0.001). The level of MEG-3 was significantly lower in the HCC group than in the cirrhotic and control groups (P < 0.001). In HCC patients, higher HOTTIP and ZEB-AS1 were linked with nodal and distant metastases, and lower MEG-3 was correlated with larger tumour size; additionally, higher HOTTIP and lower MEG-3 were associated with lower overall survival. ROC curve analysis revealed cut-off values >10.35 > 15 and < 2.3 for HOTTIP, ZEB-AS1 and MEG-3, respectively. HOTTIP, ZEB-AS1 and MEG-3 could be considered diagnostic biomarkers for HCC, and HOTTIP and MEG-3 may represent prognostic biomarkers for HCC.
ArticleNumber 100808
Author El Abd, Naglaa S.
ElTorgoman, Abdel Monem A.
Soliman, Shimaa E.
Assar, Mohamed F.A.
Gohar, Suzy F.
Girgis, Raouth E.
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Keywords Real-time PCR
Hepatocellular carcinoma
lncRNAs (HOTTIP, ZEB-AS1, MEG3)
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Snippet Hepatocellular carcinoma (HCC) is a main cause of cancer death worldwide. The high mortality of HCC patients is linked to delayed diagnosis. Long non-coding...
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elsevier
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StartPage 100808
SubjectTerms Hepatocellular carcinoma
lncRNAs (HOTTIP, ZEB-AS1, MEG3)
Real-time PCR
Title Biochemical and molecular study of long non-coding RNAs (HOTTIP, ZEB-AS1 and MEG-3) in hepatocellular carcinoma
URI https://dx.doi.org/10.1016/j.mgene.2020.100808
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