Visualizing the HoxD Gene Cluster at the Nanoscale Level

• Published in: Cold Spring Harbor Symposia on Quantitative Biology Vol. 80; p. 9
• Main Authors: Fabre, Pierre J, Benke, Alexander, Manley, Suliana, Duboule, Denis
• Format: Journal Article
• Language: English
• Published: United States 01.01.2015
• Subjects: Animals; Chromatin - metabolism; Embryonic Stem Cells; Extremities - embryology; Gene Expression Regulation, Developmental; Genes, Homeobox - genetics; Microscopy; Multigene Family; Optical Imaging

Transcription of HoxD cluster genes in limbs is coordinated by two topologically associating domains (TADs), neighboring the cluster and containing various enhancers. Here, we use a combination of microscopy approaches and chromosome conformation capture to assess the structural changes occurring in...