Enhanced production of human Cytochrome P450 2C9 by Escherichia coli BL21(DE3)pLysS through the novel use of grey relational analysis and Plackett-Burman design

• Published in: World journal of microbiology & biotechnology Vol. 23; no. 1; pp. 71 - 78
• Main Authors: Hao, Da C, Zhu, Pan H, Yang, Sheng L, Yang, Ling
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.01.2007; Springer; Springer Nature B.V
• Subjects: ammonium chloride; ampicillin; Biological and medical sciences; bioprocessing; Biotechnology; chloramphenicol; containers; Content analysis; culture media; Cytochrome; cytochrome P-450; Cytochrome P450 2C9; Design; drugs; E coli; Escherichia coli; Fundamental and applied biological sciences. Psychology; glycerol; Grey relational analysis; Heterologous expression; humans; inoculum density; magnesium sulfate; metabolism; nutrients; optimization; Peptones; Plackett-Burman design; plasmids; recombinant proteins; Statistical analysis; Statistical methods; Studies; thiamin; Trace elements; Human; Grey relational analysis; Plackett-Burman design; Cytochrome P450; Escherichia coli; Production; Bacteria; Cytochrome P450 2C9; Gene expression; Optimization; Enterobacteriaceae; Heterologous expression

Cytochrome P450 (CYP, P450) 2C9 is one of three human microsomal CYPs in subfamily 2C that contribute extensively to the hepatic metabolism of therapeutic drugs. The enhancement of recombinant CYP2C9 expression of a transformed E. coli strain is essential for the development of an efficient large-sc...