Topoisomerase II α Gene as a Marker for Prognostic Prediction of Hepatocellular Carcinoma: A Bioinformatics Analysis

• Published in: Chinese medical sciences journal Vol. 37; no. 4; pp. 331 - 339
• Main Authors: Lu, Jin, An, Shaoguang, Ma, Junjie, Yang, Yue, Zhang, Lei, Yu, Peng, Tao, Heng, Chen, Yunfan, Zhang, Haoxuan
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 31.12.2022; Anhui Key Laboratory of Computational Medicine and Intelligent Health,Bengbu Medical College,Bengbu,Anhui 233030,China%Clinical Medical College,Bengbu Medical College,Bengbu,Anhui 233030,China%Department of Otolaryngology,First Affliated Hospital,Bengbu Medical College,Bengbu,Anhui 233030,China%Department of Surgical Oncology,Second Affliated Hospital,Bengbu Medical College,Bengbu,Anhui 233030,China%School of Basic Medicine,Bengbu Medical College,Bengbu,Anhui 233030,China; School of Basic Medicine,Bengbu Medical College,Bengbu,Anhui 233030,China
• Subjects: bioinformatics analysis; Biomarkers, Tumor - genetics; Carcinoma, Hepatocellular - diagnosis; Carcinoma, Hepatocellular - genetics; CD8-Positive T-Lymphocytes; Computational Biology; disease-free survival; DNA Topoisomerases, Type II - genetics; hepatocellular carcinoma; Humans; Liver Neoplasms - diagnosis; Liver Neoplasms - genetics; overall survival; Prognosis; topoisomerase II a; overall survival; disease-free survival; topoisomerase II a; bioinformatics analysis; hepatocellular carcinoma; topoisomeraseⅡα
• DOI: 10.24920/004006
• ISSN: 1001-9294

To investigate the expression of topoisomerase II α (TOP2α) in hepatocellular carcinoma (HCC) and its role in predicting prognosis of HCC patients. We used HCC-related datasets in UALCAN, HCCDB, and cBioPortal databases to analyze the expression and mutation of TOP2α and its co-expressed genes in HCC tissues. GO function and KEGG pathway enrichment of TOP2α and its co-expressed genes were identified. The TIMER database was used to analyze infiltration levels of immune cells in HCC. The impacts of TOP2α and its co-expression genes and the infiltrated immune cells on the survival of HCC patients were assayed by Kaplan-Meier plotter analysis. TOP2α and its co-expression genes were highly expressed in HCC (P < 0.001) and detrimental to overall survival of HCC patients (P < 0.001). TOP2α and its co-expression genes were mainly involved in cell mitosis and proliferation, and cell cycle pathway (ID: hsa04110, P = 0.00194S). TOP2α and its co-expression genes were mutated in HCC and the mutations were significantly detrimental to overall survival (P = 0.0247) and disease-free survival (P = 0.026S) of HCC patients. High TOP2α expression was positively correlated with the infiltration of B cell (r = 0.4S9, P < 0.01), CD8+T cell (r = 0.312, P < 0.01), CD4+T cell (r = 0.370, P < 0.01), macrophage (r = 0.459, P < 0.01), neutrophil (r = 0.405, P < 0.01), and dendritic cell (r = 0.473, P < 0.01) in HCC. The CD8+T cell infiltration significantly prolonged the 3- and 5-year survival of HCC patients (all P < 0.05), and CD4+T cell infiltration significantly shortened the 3-, 5-, and 10-year survival of HCC patients (all P < 0.05) TOP2α may be an oncogene, which was associated with poor prognosis of HCC patients and could be used as a biomarker for the prognostic prediction of HCC.