Human factor VIII procoagulant activity and phospholipid interaction

• Published in: Biochimica et biophysica acta Vol. 678; no. 1; pp. 132 - 136
• Main Authors: Lajmanovich, A, Hudry-Clergeon, G, Freyssinet, J M, Marguerie, G
• Format: Journal Article
• Language: English
• Published: Netherlands 18.11.1981
• Subjects: Antigens - metabolism; Centrifugation, Density Gradient; Factor VIII - immunology; Factor VIII - isolation & purification; Factor VIII - metabolism; Humans; Liposomes; Partial Thromboplastin Time; Phosphatidylcholines; Phosphatidylethanolamines; Phospholipids; von Willebrand Factor - metabolism

The interaction between purified human factor VIII and phospholipid vesicles was investigated. The binding of factor VIII to an equimolecular mixture of phosphatidylserine (PS) and phosphatidylcholine (PC) was studied by sucrose gradient ultracentrifugation (10-40% w/v saccharose in 0.01 M Tris-HC1/...