Fatty Acid Synthesis of an Eicosapentaenoic Acid-Producing Bacterium: De Novo Synthesis, Chain Elongation, and Desaturation Systems
The fatty acid synthesis systems of a Shewanella sp., strain SCRC-2738, that produces a large amount of eicosapentaenoic acid were investigated. Two kinds of fatty acid synthesis system, de novo synthesis and chain elongation ones, were detected in the cytosol. The de novo synthesis system required...
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Published in | Journal of biochemistry (Tokyo) Vol. 122; no. 2; pp. 467 - 473 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
01.08.1997
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Subjects | |
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Abstract | The fatty acid synthesis systems of a Shewanella sp., strain SCRC-2738, that produces a large amount of eicosapentaenoic acid were investigated. Two kinds of fatty acid synthesis system, de novo synthesis and chain elongation ones, were detected in the cytosol. The de novo synthesis system required an acyl carrier protein, and produced palmitoyl- and palmitoleoyl-acyl carrier proteins as final products. The chain elongation system also required an acyl carrier protein, and produced an acyl-acyl carrier protein as a product, using palmitoyl-, palmitoleoyl-, stearoyl-, and oleoyl-CoAs as primers but not eicosanoyl-or eicosenoyl-CoA. There were an anaerobic pathway and an aerobic desaturation one for the production of unsaturated fatty acids. Eicosapentaenoic acid seemed to be produced through the aerobic desaturation pathway and not through the anaerobic one, since the latter pathway produced n-7 type monoenoic fatty acids, which are different from eicosapentaenoic acid in the position of the double bond. The desaturase utilized an acyl-acyl carrier protein as a substrate, and this activity increased in the presence of ferredoxin and ferredoxin NADP+ reductase. Thus, Shewanella sp., strain SCRC-2738, has novel characteristics as to both fatty acid chain elongation and desaturation systems. |
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AbstractList | The fatty acid synthesis systems of a Shewanella sp., strain SCRC-2738, that produces a large amount of eicosapentaenoic acid were investigated. Two kinds of fatty acid synthesis system, de novo synthesis and chain elongation ones, were detected in the cytosol. The de novo synthesis system required an acyl carrier protein, and produced palmitoyl- and palmitoleoyl-acyl carrier proteins as final products. The chain elongation system also required an acyl carrier protein, and produced an acyl-acyl carrier protein as a product, using palmitoyl-, palmitoleoyl-, stearoyl-, and oleoyl-CoAs as primers but not eicosanoyl- or eicosenoyl-CoA. There were an anaerobic pathway and an aerobic desaturation one for the production of unsaturated fatty acids. Eicosapentaenoic acid seemed to be produced through the aerobic desaturation pathway and not through the anaerobic one, since the latter pathway produced n-7 type monoenoic fatty acids, which are different from eicosapentaenoic acid in the position of the double bond. The desaturase utilized an acyl-acyl carrier protein as a substrate, and this activity increased in the presence of ferredoxin and ferredoxin NADP+ reductase. Thus, Shewanella sp., strain SCRC-2738, has novel characteristics as to both fatty acid chain elongation and desaturation systems. |
Author | Kondo, Kiyosi Watanabe, Kazuo Yazawa, Kazunaga Kawaguchi, Akihiko |
Author_xml | – sequence: 1 givenname: Kazuo surname: Watanabe fullname: Watanabe, Kazuo organization: Sagami Chemical Research Center Nishi-Ohnuma 4-4-1, Sagamihara, Kanagawa 229 – sequence: 2 givenname: Kazunaga surname: Yazawa fullname: Yazawa, Kazunaga organization: Sagami Chemical Research Center Nishi-Ohnuma 4-4-1, Sagamihara, Kanagawa 229 – sequence: 3 givenname: Kiyosi surname: Kondo fullname: Kondo, Kiyosi organization: Sagami Chemical Research Center Nishi-Ohnuma 4-4-1, Sagamihara, Kanagawa 229 – sequence: 4 givenname: Akihiko surname: Kawaguchi fullname: Kawaguchi, Akihiko organization: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo Komaba 3-8-1, Meguro-ku, Tokyo 153 |
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Snippet | The fatty acid synthesis systems of a Shewanella sp., strain SCRC-2738, that produces a large amount of eicosapentaenoic acid were investigated. Two kinds of... |
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SubjectTerms | Acetyl Coenzyme A - metabolism Acyl Carrier Protein - pharmacology Cerulenin - pharmacology de novo fatty acid synthesis eicosapentaenoic acid Eicosapentaenoic Acid - biosynthesis Enzyme Inhibitors - pharmacology fatty acid chain elongation Fatty Acid Desaturases - metabolism fatty acid desaturation Fatty Acids - biosynthesis Fatty Acids - metabolism Gram-Negative Facultatively Anaerobic Rods - metabolism Malonyl Coenzyme A - metabolism marine bacterium Models, Chemical NAD - pharmacology NADP - pharmacology |
Title | Fatty Acid Synthesis of an Eicosapentaenoic Acid-Producing Bacterium: De Novo Synthesis, Chain Elongation, and Desaturation Systems |
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