A sustainable mouse karyotype created by programmed chromosome fusion

• Published in: Science (American Association for the Advancement of Science) Vol. 377; no. 6609; pp. 967 - 975
• Main Authors: Wang, Li-Bin, Li, Zhi-Kun, Wang, Le-Yun, Xu, Kai, Ji, Tian-Tian, Mao, Yi-Huan, Ma, Si-Nan, Liu, Tao, Tu, Cheng-Fang, Zhao, Qian, Fan, Xu-Ning, Liu, Chao, Wang, Li-Ying, Shu, You-Jia, Yang, Ning, Zhou, Qi, Li, Wei
• Format: Journal Article
• Language: English
• Published: Washington The American Association for the Advancement of Science 26.08.2022
• Subjects: Chromosomes; Deoxyribonucleic acid; DNA; Embryo cells; Gene sequencing; Genetic engineering; Genetic modification; Karyotypes; Nucleotide sequence; Stem cell transplantation; Stem cells; Yeast

Chromosome engineering has been attempted successfully in yeast but remains challenging in higher eukaryotes, including mammals. Here, we report programmed chromosome ligation in mice that resulted in the creation of new karyotypes in the lab. Using haploid embryonic stem cells and gene editing, we...