FGF2-induced PI3K/Akt signaling evokes greater proliferation and adipogenic differentiation of human adipose stem cells from breast than from abdomen or thigh
In this study, human adipose stem cells were isolated from subcutaneous fat in the thigh (htASCs), abdomen (haASCs) and breast (hbASCs). Flow cytometry was used to detect cell surface markers, and an enzyme-linked immunosorbent assay was used to detect paracrine activity. Paracrine gene expression i...
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| Published in | Aging (Albany, NY.) Vol. 12; no. 14; pp. 14830 - 14848 |
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| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Impact Journals
24.07.2020
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| Online Access | Get full text |
| ISSN | 1945-4589 1945-4589 |
| DOI | 10.18632/aging.103547 |
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| Abstract | In this study, human adipose stem cells were isolated from subcutaneous fat in the thigh (htASCs), abdomen (haASCs) and breast (hbASCs). Flow cytometry was used to detect cell surface markers, and an enzyme-linked immunosorbent assay was used to detect paracrine activity. Paracrine gene expression in the three cell types was examined using real-time qPCR, and adipogenic ability was assessed using Oil Red O staining. RNA from third-passage haASCs and hbASCs was sequenced. The results showed that the differentiation potential marker markers CD49d and CD54 were similar across hbASCs from 10 subjects. The hbASCs showed higher colony forming ability and expression of fibroblast growth factor-2, tissue inhibitor of metalloproteinase-1 and stromal cell derived factor-1 than htASCs and haASCs. Stimulating hbASCs with FGF2 promoted adipogenic differentiation, while treating the cells with the PI3K inhibitor LY294002 inhibited differentiation. These results suggest that the PI3K/Akt signaling pathway can promote proliferation and adipogenic differentiation of adipose stem cells, and that activation of this pathway by FGF2 may explain why hbASCs show greater proliferation and adipogenic differentiation than haASCs and htASCs. |
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| AbstractList | In this study, human adipose stem cells were isolated from subcutaneous fat in the thigh (htASCs), abdomen (haASCs) and breast (hbASCs). Flow cytometry was used to detect cell surface markers, and an enzyme-linked immunosorbent assay was used to detect paracrine activity. Paracrine gene expression in the three cell types was examined using real-time qPCR, and adipogenic ability was assessed using Oil Red O staining. RNA from third-passage haASCs and hbASCs was sequenced. The results showed that the differentiation potential marker markers CD49d and CD54 were similar across hbASCs from 10 subjects. The hbASCs showed higher colony forming ability and expression of fibroblast growth factor-2, tissue inhibitor of metalloproteinase-1 and stromal cell derived factor-1 than htASCs and haASCs. Stimulating hbASCs with FGF2 promoted adipogenic differentiation, while treating the cells with the PI3K inhibitor LY294002 inhibited differentiation. These results suggest that the PI3K/Akt signaling pathway can promote proliferation and adipogenic differentiation of adipose stem cells, and that activation of this pathway by FGF2 may explain why hbASCs show greater proliferation and adipogenic differentiation than haASCs and htASCs.In this study, human adipose stem cells were isolated from subcutaneous fat in the thigh (htASCs), abdomen (haASCs) and breast (hbASCs). Flow cytometry was used to detect cell surface markers, and an enzyme-linked immunosorbent assay was used to detect paracrine activity. Paracrine gene expression in the three cell types was examined using real-time qPCR, and adipogenic ability was assessed using Oil Red O staining. RNA from third-passage haASCs and hbASCs was sequenced. The results showed that the differentiation potential marker markers CD49d and CD54 were similar across hbASCs from 10 subjects. The hbASCs showed higher colony forming ability and expression of fibroblast growth factor-2, tissue inhibitor of metalloproteinase-1 and stromal cell derived factor-1 than htASCs and haASCs. Stimulating hbASCs with FGF2 promoted adipogenic differentiation, while treating the cells with the PI3K inhibitor LY294002 inhibited differentiation. These results suggest that the PI3K/Akt signaling pathway can promote proliferation and adipogenic differentiation of adipose stem cells, and that activation of this pathway by FGF2 may explain why hbASCs show greater proliferation and adipogenic differentiation than haASCs and htASCs. In this study, human adipose stem cells were isolated from subcutaneous fat in the thigh (htASCs), abdomen (haASCs) and breast (hbASCs). Flow cytometry was used to detect cell surface markers, and an enzyme-linked immunosorbent assay was used to detect paracrine activity. Paracrine gene expression in the three cell types was examined using real-time qPCR, and adipogenic ability was assessed using Oil Red O staining. RNA from third-passage haASCs and hbASCs was sequenced. The results showed that the differentiation potential marker markers CD49d and CD54 were similar across hbASCs from 10 subjects. The hbASCs showed higher colony forming ability and expression of fibroblast growth factor-2, tissue inhibitor of metalloproteinase-1 and stromal cell derived factor-1 than htASCs and haASCs. Stimulating hbASCs with FGF2 promoted adipogenic differentiation, while treating the cells with the PI3K inhibitor LY294002 inhibited differentiation. These results suggest that the PI3K/Akt signaling pathway can promote proliferation and adipogenic differentiation of adipose stem cells, and that activation of this pathway by FGF2 may explain why hbASCs show greater proliferation and adipogenic differentiation than haASCs and htASCs. |
| Author | Ma, Yan-Fei Luo, Zhi-Zhai Mo, Steven Rong, Yong-Xian Li, Hong-Mian Liang, Zhi-Jie Wu, Fang-Xiao Hunag, Dong-Lin Lu, Guan-Ming Liu, Xin-Heng |
| Author_xml | – sequence: 1 givenname: Guan-Ming surname: Lu fullname: Lu, Guan-Ming organization: Department of Breast and Thyroid Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Guangxi 533000, China – sequence: 2 givenname: Yong-Xian surname: Rong fullname: Rong, Yong-Xian organization: Department of Burn and Plastic Surgery, Guiping People’s Hospital, Guigping 537200, Guangxi, China – sequence: 3 givenname: Zhi-Jie surname: Liang fullname: Liang, Zhi-Jie organization: Department of Plastic and Aesthetic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University and The First People’s Hospital of Nanning, Nanning 530022, Guangxi, China – sequence: 4 givenname: Dong-Lin surname: Hunag fullname: Hunag, Dong-Lin organization: Department of Plastic and Aesthetic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University and The First People’s Hospital of Nanning, Nanning 530022, Guangxi, China – sequence: 5 givenname: Fang-Xiao surname: Wu fullname: Wu, Fang-Xiao organization: Department of Plastic and Aesthetic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University and The First People’s Hospital of Nanning, Nanning 530022, Guangxi, China – sequence: 6 givenname: Yan-Fei surname: Ma fullname: Ma, Yan-Fei organization: Department of Breast and Thyroid Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Guangxi 533000, China – sequence: 7 givenname: Zhi-Zhai surname: Luo fullname: Luo, Zhi-Zhai organization: Department of Breast and Thyroid Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Guangxi 533000, China – sequence: 8 givenname: Xin-Heng surname: Liu fullname: Liu, Xin-Heng organization: Department of Burn and Plastic Surgery, Guiping People’s Hospital, Guigping 537200, Guangxi, China – sequence: 9 givenname: Steven surname: Mo fullname: Mo, Steven organization: Nanning Life-Ontology Biotechnology Co., Ltd., Nanning 530229, Guangxi, China – sequence: 10 givenname: Hong-Mian surname: Li fullname: Li, Hong-Mian organization: Department of Plastic and Aesthetic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University and The First People’s Hospital of Nanning, Nanning 530022, Guangxi, China |
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| Keywords | adipose-derived stem cells molecular signature depot-specific stem cell populations adipogenic differentiation paracrine |
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| Snippet | In this study, human adipose stem cells were isolated from subcutaneous fat in the thigh (htASCs), abdomen (haASCs) and breast (hbASCs). Flow cytometry was... |
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| SubjectTerms | Abdomen - pathology Adipocytes - metabolism Adipogenesis - physiology Breast - pathology Cell Differentiation - physiology Fibroblast Growth Factor 2 - metabolism Humans Mesenchymal Stem Cells - metabolism Paracrine Communication - physiology Phosphatidylinositol 3-Kinases - metabolism Proto-Oncogene Proteins c-akt - metabolism Research Paper Signal Transduction Subcutaneous Fat - cytology Subcutaneous Fat - metabolism Thigh - pathology |
| Title | FGF2-induced PI3K/Akt signaling evokes greater proliferation and adipogenic differentiation of human adipose stem cells from breast than from abdomen or thigh |
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