Benefits of Intraluminal Injection of Glutamine for Intestinal Mucosa during Ischemia-Reperfusion

• Published in: European surgical research Vol. 35; no. 4; pp. 352 - 356
• Main Authors: de Aguilar-Nascimento, J.E., Gurgel Marques, Cláudia, Carvalho Mariano, Alessandra, Bicudo Salomão, A., de Souza Neves, J.
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.07.2003; S. Karger AG
• Subjects: Animals; Biological and medical sciences; Disease Models, Animal; Gastroenterology. Liver. Pancreas. Abdomen; Glutamine - pharmacology; Ileum - pathology; Intestinal Mucosa - pathology; Jejunum - pathology; Leukocyte Count; Medical sciences; Neutrophils - pathology; Original Paper; Other diseases. Semiology; Rats; Rats, Wistar; Reperfusion Injury - drug therapy; Reperfusion Injury - pathology; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Reperfusion; Ischemia; Small intestine; Mucosa; Neutrophils; Glutamine; Animal model; Rat; Treatment efficiency; Rodentia; Gut; Lamina propria; Cardiovascular disease; Experimental study; Intraluminal; Vertebrata; Mammalia; Cytoprotector; Aminoacid; Animal; Neutrophil; Comparative study
• ISSN: 0014-312X; 1421-9921
• DOI: 10.1159/000070606

Background: We investigated the effect of intraluminal glutamine on the intestinal mucosa in the presence of ischemia-reperfusion injury (IRI). Methods: Six blind sacs were created in the small bowel (3 at the jejunum and 3 at the ileum) in 10 Wistar rats. The lateral sacs of both bowel regions were submitted to IRI (30/30 min), while the medial sacs were left free to receive blood supply. In the lateral sacs, a solution containing either saline plus 4% glutamine or pure saline was injected at the bowel lumen. No fluid was injected in the medial sacs. Results: Both at the jejunum and at the ileum, the score of the mucosal injury was higher in saline-injected sacs than in either glutamine-injected or control sacs. There was a significantly greater number of neutrophils in the sacs treated with saline than in the other two groups of sacs. Conclusion: Glutamine protects the mucosa and diminishes the accumulation of neutrophils at the lamina propria of the small bowel in IRI.