Comparison of isometric and isotonic responses of human small airway smooth muscle in vitro

The difference between isometric and isotonic responses of human small airway smooth muscle to a number of pharmacological agonists was studied. The isotonically measured sensitivity to methacholine was 1.4 times less than the isometrically measured value (p less than 0.05), and similar small discre...

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Bibliographic Details
Published inJournal of pharmacological methods Vol. 17; no. 2; p. 165
Main Authors de Jongste, J C, Mons, H, van Strik, R, Bonta, I L, Kerrebijn, K F
Format Journal Article
LanguageEnglish
Published United States 01.04.1987
Subjects
Bronchi - physiology
Dose-Response Relationship, Drug
Humans
In Vitro Techniques
Methacholine Chloride
Methacholine Compounds - pharmacology
Muscle Contraction - drug effects
Muscle, Smooth - physiology
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Summary:The difference between isometric and isotonic responses of human small airway smooth muscle to a number of pharmacological agonists was studied. The isotonically measured sensitivity to methacholine was 1.4 times less than the isometrically measured value (p less than 0.05), and similar small discrepancies were found for histamine, leukotriene, prostaglandin F2 alpha, isoproterenol, and theophylline. Maximal isometric force and isotonic shortening after methacholine were linearly related (p less than 0.01). The between-methods difference is relatively small. Because the difference was of similar magnitude and in the same direction in all tissues studied, it is of little practical importance for conventional pharmacological experiments.
ISSN:0160-5402
DOI:10.1016/0160-5402(87)90027-1