Somatic hybrids of Sinapis alba + Brassica juncea: study of backcross progenies for morphological variations, chromosome constitution and reaction to Alternaria brassicae
The wild relatives of crops represent a rich reservoir of genes for introducing resilience to climate change into cultivated crops. To introgress genes from Sinapis alba , a wild relative of Brassicaceae, into Brassica juncea , a cultivated member of this family, we initially produced somatic hybrid...
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Published in | Euphytica Vol. 216; no. 6 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.06.2020
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The wild relatives of crops represent a rich reservoir of genes for introducing resilience to climate change into cultivated crops. To introgress genes from
Sinapis alba
, a wild relative of Brassicaceae, into
Brassica juncea
, a cultivated member of this family, we initially produced somatic hybrids between the two species and then produced a large number of backcross populations involving the two somatic hybrids (H1 and H2) with
Brassica juncea
. BC
1
progeny were morphologically very similar. However, when they were challenge inoculated with a highly virulent
Alternaria brassicae
(ITCC No. 2542) culture under in vivo and in vitro conditions in two growing seasons, they showed wide variations in their disease reaction. Of the 40 BC
1
lines tested in one season, 36 showed a resistant reaction. BC
1
F
2
progenies derived from these resistant BC
1
plants also showed resistance to
Alternaria brassicae
, indicating stable inheritance of the resistant phenotype. However, BC
1
F
2
progenies showed a wide variation in morphological traits, including plant height, basal branching, leaf thickness, trichome density on leaves and stem. BC
1
plants were examined by genomic in-situ hybridization (GISH) to determine their chromosome constitution. All five plants were found to possess 12 strong hybridization signals upon hybridization with a FITC-labeled
S. alba
-specific probe. GISH studies on BC
1
F
2
plants indicated localized signals in addition to 12 full chromosome hybridization signals, suggesting alien introgressions into
B. juncea
that requires further validation. The BC
2
generation was found to possess half of the haploid set of alien chromosomes. The BC
1
F
2
and BC
2
generations were further screened against
A. Brassiceae
and found to be resistant/tolerant. |
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ISSN: | 0014-2336 1573-5060 |
DOI: | 10.1007/s10681-020-02629-3 |