Cellulase-free-thermo-alkali-solvent-stable xylanase from Bacillus altitudinis DHN8: Over-production through statistical approach, purification and bio-deinking/ bio-bleaching potential

The present study shows over-production of cellulase-free xylanase using Bacillus altitudinis DHN8 followed by its purification and applications. Response surface methodology, especially Box-Behnken Design was used to improve the enzyme yield by optimizing submerged fermentation conditions. Statisti...

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Published inBiocatalysis and agricultural biotechnology Vol. 12; pp. 220 - 227
Main Authors Adhyaru, Dharmesh N., Bhatt, Nikhil S., Modi, H.A., Divecha, Jyoti
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.10.2017
Subjects
Bio-bleaching
Bio-deinking
Cellulase-free xylanase
Over-production
Purification
Response surface methodology
Cellulase-free xylanase
Purification
Bio-deinking
Response surface methodology
Over-production
Bio-bleaching
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Abstract The present study shows over-production of cellulase-free xylanase using Bacillus altitudinis DHN8 followed by its purification and applications. Response surface methodology, especially Box-Behnken Design was used to improve the enzyme yield by optimizing submerged fermentation conditions. Statistically optimized fermentation conditions to achieve higher xylanase production (492IU/mL) were sorghum straw 1.0%, inoculum size 3.0%, incubation time 48h, temperature 32°C, agitation speed 200rpm and gelatin 0.37%. Under these conditions, the resulted enzyme activity was two-fold high compared to the activity (245IU/mL) resulted after one-factor-at-a-time optimization. The purified xylanase was of high molecular weight (~98kDa) as revealed by SDS-PAGE. It showed optimum activity between 45°C and 55°C and at pH 8.0. Various metals, modulators, and organic solvents further improved the activity of purified enzyme. In this study, the crude xylanase was used for (1) bio-deinking of hand-written office waste papers having blue ink and (2) bio-bleaching of carton pulp. In the first case, 56.3% deinking of waste papers was achieved whereas in the second case, Kappa number of carton pulp was reduced to 10.85. Both results are suggesting the potential of B. altitudinis xylanase for the bio-deinking and bio-bleaching. [Display omitted] •Cellulase-free xylanase was over-produced from Bacillus altitudinis DHN8.•Statistical optimization through BBD has improved the xylanase yield markedly.•Very rare high molecular weight and robust xylanase was purified in active form.•Application opportunities of xylanase in bio-deinking and bio-bleaching.
AbstractList The present study shows over-production of cellulase-free xylanase using Bacillus altitudinis DHN8 followed by its purification and applications. Response surface methodology, especially Box-Behnken Design was used to improve the enzyme yield by optimizing submerged fermentation conditions. Statistically optimized fermentation conditions to achieve higher xylanase production (492IU/mL) were sorghum straw 1.0%, inoculum size 3.0%, incubation time 48h, temperature 32°C, agitation speed 200rpm and gelatin 0.37%. Under these conditions, the resulted enzyme activity was two-fold high compared to the activity (245IU/mL) resulted after one-factor-at-a-time optimization. The purified xylanase was of high molecular weight (~98kDa) as revealed by SDS-PAGE. It showed optimum activity between 45°C and 55°C and at pH 8.0. Various metals, modulators, and organic solvents further improved the activity of purified enzyme. In this study, the crude xylanase was used for (1) bio-deinking of hand-written office waste papers having blue ink and (2) bio-bleaching of carton pulp. In the first case, 56.3% deinking of waste papers was achieved whereas in the second case, Kappa number of carton pulp was reduced to 10.85. Both results are suggesting the potential of B. altitudinis xylanase for the bio-deinking and bio-bleaching. [Display omitted] •Cellulase-free xylanase was over-produced from Bacillus altitudinis DHN8.•Statistical optimization through BBD has improved the xylanase yield markedly.•Very rare high molecular weight and robust xylanase was purified in active form.•Application opportunities of xylanase in bio-deinking and bio-bleaching.
Author Adhyaru, Dharmesh N.
Bhatt, Nikhil S.
Divecha, Jyoti
Modi, H.A.
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Keywords Cellulase-free xylanase
Purification
Bio-deinking
Response surface methodology
Over-production
Bio-bleaching
Language English
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Snippet The present study shows over-production of cellulase-free xylanase using Bacillus altitudinis DHN8 followed by its purification and applications. Response...
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SubjectTerms Bio-bleaching
Bio-deinking
Cellulase-free xylanase
Over-production
Purification
Response surface methodology
Title Cellulase-free-thermo-alkali-solvent-stable xylanase from Bacillus altitudinis DHN8: Over-production through statistical approach, purification and bio-deinking/ bio-bleaching potential
URI https://dx.doi.org/10.1016/j.bcab.2017.10.010
Volume 12
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