Dependence of synchronized bursting activity on medium stirring and the perfusion rate in a cultured network of neurons
A cultured network of neurons coupled with a multi-electrode-array (MEA) recording system has been a useful platform for investigating various issues in neuroscience and engineering. The neural activity supported by the system can be sensitive to environmental fluctuations, for example, in the mediu...
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Published in | Journal of the Korean Physical Society Vol. 68; no. 9; pp. 1041 - 1048 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
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Seoul
The Korean Physical Society
01.05.2016
한국물리학회 |
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Abstract | A cultured network of neurons coupled with a multi-electrode-array (MEA) recording system has been a useful platform for investigating various issues in neuroscience and engineering. The neural activity supported by the system can be sensitive to environmental fluctuations, for example, in the medium’s nutrient composition, ph, and temperature, and to mechanical disturbances, yet this issue has not been the subject. Especially, a normal practice in maintaining neuronal cell cultures involves an intermittent sequence of medium exchanges, typically at a time interval of a few days, and one such sudden medium exchange is unavoidably accompanied by many unintended disturbances. Here, based on a quantitative time-series analysis of synchronized bursting events, we explicitly demonstrate that such a medium exchange can, indeed, bring a huge change in the existing neural activity. Subsequently, we develop a medium perfusion-stirring system and an ideal protocol that can be used in conjunction with a MEA recording system, providing long-term stability. Specifically, we systematically evaluate the effects of medium stirring and perfusion rates. Unexpectedly, even some vigorous mechanical agitations do not have any impacts on neural activity. On the other hand, too much replenishment (
e.g.
, 1.8 ml/day for a 1.8-ml dish) of neurobasal medium results in an excitotoxicity. |
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AbstractList | A cultured network of neurons coupled with a multi-electrode-array (MEA) recording system has been a useful platform for investigating various issues in neuroscience and engineering. The neural activity supported by the system can be sensitive to environmental fluctuations, for example, in the medium’s nutrient composition, ph, and temperature, and to mechanical disturbances, yet this issue has not been the subject. Especially, a normal practice in maintaining neuronal cell cultures involves an intermittent sequence of medium exchanges, typically at a time interval of a few days, and one such sudden medium exchange is unavoidably accompanied by many unintended disturbances.
Here, based on a quantitative time-series analysis of synchronized bursting events, we explicitly demonstrate that such a medium exchange can, indeed, bring a huge change in the existing neural activity. Subsequently, we develop a medium perfusion-stirring system and an ideal protocol that can be used in conjunction with a MEA recording system, providing long-term stability. Specifically, we systematically evaluate the effects of medium stirring and perfusion rates. Unexpectedly, even some vigorous mechanical agitations do not have any impacts on neural activity. On the other hand, too much replenishment (e.g., 1.8 ml/day for a 1.8-ml dish) of neurobasal medium results in an excitotoxicity. KCI Citation Count: 0 A cultured network of neurons coupled with a multi-electrode-array (MEA) recording system has been a useful platform for investigating various issues in neuroscience and engineering. The neural activity supported by the system can be sensitive to environmental fluctuations, for example, in the medium’s nutrient composition, ph, and temperature, and to mechanical disturbances, yet this issue has not been the subject. Especially, a normal practice in maintaining neuronal cell cultures involves an intermittent sequence of medium exchanges, typically at a time interval of a few days, and one such sudden medium exchange is unavoidably accompanied by many unintended disturbances. Here, based on a quantitative time-series analysis of synchronized bursting events, we explicitly demonstrate that such a medium exchange can, indeed, bring a huge change in the existing neural activity. Subsequently, we develop a medium perfusion-stirring system and an ideal protocol that can be used in conjunction with a MEA recording system, providing long-term stability. Specifically, we systematically evaluate the effects of medium stirring and perfusion rates. Unexpectedly, even some vigorous mechanical agitations do not have any impacts on neural activity. On the other hand, too much replenishment ( e.g. , 1.8 ml/day for a 1.8-ml dish) of neurobasal medium results in an excitotoxicity. |
Author | Kim, Hyun Heo, Ryoun Lee, Kyoung J. |
Author_xml | – sequence: 1 givenname: Ryoun surname: Heo fullname: Heo, Ryoun organization: Department of Physics, Korea University – sequence: 2 givenname: Hyun surname: Kim fullname: Kim, Hyun organization: Department of Physics, Korea University – sequence: 3 givenname: Kyoung J. surname: Lee fullname: Lee, Kyoung J. email: kyoung@korea.ac.kr organization: Department of Physics, Korea University |
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Cites_doi | 10.1103/PhysRevLett.108.138103 10.1371/journal.pone.0025633 10.1186/1471-2202-7-11 10.2144/05382RR03 10.1103/PhysRevLett.88.118102 10.1088/1742-5468/2014/04/P04019 10.1016/0165-0270(94)90059-0 10.3389/fphys.2014.00043 10.1109/TBME.2004.827936 10.1074/jbc.M111.253740 10.1016/S0165-022X(01)00163-4 10.1152/jn.2000.83.2.828 10.1098/rsos.150031 10.1103/PhysRevE.92.042701 10.1113/jphysiol.1996.sp021417 10.1016/j.brainres.2006.03.049 10.1016/0896-6273(94)90032-9 10.1016/j.neunet.2008.06.008 10.1038/srep01081 10.1097/WNR.0000000000000343 10.1002/eej.10216 10.1016/j.jneumeth.2006.09.017 10.1523/JNEUROSCI.19-06-02102.1999 10.1016/0165-0270(88)90116-1 10.1016/S0956-5663(03)00092-7 |
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Keywords | Synchronized neural burst Long-term neural recording Perfusion system for neuronal cell culture Multi-electrode array Effect of mechanical stirring on neural activity |
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Title | Dependence of synchronized bursting activity on medium stirring and the perfusion rate in a cultured network of neurons |
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