The Chemical Synthesis of Relaxin and Related Peptides

• Published in: Annals of the New York Academy of Sciences Vol. 1160; no. 1; pp. 11 - 15
• Main Authors: Wade, John D., Lin, Feng, Hossain, M. Akhter, Shabanpoor, Fazel, Zhang, Suode, Tregear, Geoffrey W.
• Format: Journal Article
• Language: English
• Published: Malden, USA Blackwell Publishing Inc 01.04.2009
• Subjects: Animals; chemical synthesis; Humans; insulin-like peptide; Peptides - chemical synthesis; Peptides - chemistry; regioselective disulfide bond formation; relaxin; Relaxin - analogs & derivatives; Relaxin - chemical synthesis; Relaxin - chemistry; solid-phase peptide synthesis
• ISSN: 0077-8923; 1749-6632
• DOI: 10.1111/j.1749-6632.2009.03951.x

Successful methods for the chemical assembly of insulin‐like peptides allow the detailed study of their structure and function relationships. However, the two‐chain, three‐disulfide bond structure of this family of peptides, which includes relaxin, has long represented a significant challenge with respect to their chemical synthesis. Early efforts involved the random combination of the two synthetic S‐reduced chains under oxidizing conditions to spontaneously form the three disulfide bonds. Such an approach, while generally effective for native sequences, is critically dependent upon the presence of intact secondary structures within the individual chains which guide the subsequent folding and oxidation pathway. This limitation prevents the use of this approach for the preparation of analogs in which these secondary elements are either absent or modified. Nowadays, the use of highly efficient solid‐phase peptide synthesis methodologies together with selective S‐thiol‐protecting groups allows the acquisition of individual chains that can be combined by effective sequential chemically directed formation of each of the three disulfide bonds. These approaches have allowed the high‐yield assembly of an array of insulin‐like peptides which, in turn, has provided considerable and valuable structural and biological information.