Multiple analysis of three common genetic alterations associated with thrombophilia

• Published in: Blood coagulation & fibrinolysis Vol. 11; no. 2; p. 183
• Main Authors: Pecheniuk, N M, Marsh, N A, Walsh, T P
• Format: Journal Article
• Language: English
• Published: England 01.03.2000
• Subjects: Adenine - analysis; Australia; Biotechnology; DNA - blood; Factor V - genetics; Genetic Variation; Genotype; Guanine - analysis; Heterozygote; Humans; Methylenetetrahydrofolate Reductase (NADPH2); Oxidoreductases Acting on CH-NH Group Donors - genetics; Point Mutation; Polymerase Chain Reaction; Spectrophotometry; Thrombophilia - genetics; Venous Thrombosis - genetics; Australia

We have previously reported the use of a novel mini-sequencing protocol for detection of the factor V Leiden variant, the first nucleotide change (FNC) technology. This technology is based on a single nucleotide extension of a primer, which is hybridized immediately adjacent to the site of mutation....