High sensitivity PCR assay in plastic micro reactors

• Published in: Lab on a chip Vol. 2; no. 4; p. 179
• Main Authors: Yang, Jianing, Liu, Yingjie, Rauch, Cory B., Stevens, Randall L., Liu, Robin H., Lenigk, Ralf, Grodzinski, Piotr
• Format: Journal Article
• Language: English
• Published: England 01.11.2002
• Subjects: Base Sequence; DNA Primers; Escherichia coli - genetics; Genes, Bacterial; Plastics; Polycarboxylate Cement - chemistry; Polymerase Chain Reaction - instrumentation; Sensitivity and Specificity
• ISSN: 1473-0197; 1473-0189
• DOI: 10.1039/b208405h

Small volume operation and rapid thermal cycling have been subjects of numerous reports in micro reactor chip development. Sensitivity aspects of the micro PCR reactor have not been studied in detail, however, despite the fact that detection of rare targets or trace genomic material from clinical an...