A detailed structural characterization of ribonuclease B oligosaccharides by 1H NMR spectroscopy and mass spectrometry

• Published in: Carbohydrate research Vol. 261; no. 2; pp. 173 - 186
• Main Authors: Fu, Daotian, Chen, Ling, O'Neill, Roger A.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 17.08.1994; Elsevier Science
• Subjects: Amidohydrolases; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Carbohydrate Sequence; Cattle; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Glycosylation; Hydrolases; Magnetic Resonance Spectroscopy; Mass Spectrometry; Molecular Sequence Data; NMR spectroscopy; Oligosaccharides; Oligosaccharides - chemistry; Oxidation-Reduction; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; Ribonuclease B; Ribonucleases - chemistry; Oligosaccharides; NMR spectroscopy; Mass spectrometry; Ribonuclease B; Bovine; Enzyme; Oligosaccharide; Esterases; NMR spectrometry; Vertebrata; Mammalia; Hydrolases; Chemical composition; Artiodactyla; Pancreatic ribonuclease; Ungulata; Structural analysis

The structures of ribonuclease B oligosaccharides have previously been shown to be high mannose type by methylation analyses and sequential exoglycosidase digestion. Due to the unique nature of these oligosaccharides, in that all mannosyl residues are attached by α-(1 → 2)-linkages beyond the branch...