Estimation of [ 32P]deoxyribonucleoside triphosphates in cell extracts using periodate treatment

• Published in: Analytical biochemistry Vol. 82; no. 2; pp. 591 - 595
• Main Authors: Reynolds, Eric C., Finch, Lloyd R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.1977
• Subjects: Cell Line; Chromatography, Thin Layer - methods; Deoxyribonucleotides - analysis; Oxidation-Reduction; Periodic Acid

Radioisotopic labeling of nucleotides in cells before extraction, coupled with two-dimensional chromatography on PEI-cellulose 1 1 Abbreviations: dNTP, deoxyribonucleoside triphosphate(s); rNTP, ribonucleoside triphosphate(s); PEI, polyethyleneimine. thin layers (1,2), has been successfully used in...