Role of enhanced arachidonate availability through phospholipase A2 pathway in mediation of increased prostaglandin synthesis by glomeruli from diabetic rats
Prostaglandin E2 (PGE2) production by superfused glomeruli from rats made diabetic for 2 wk by streptozocin injection is twofold higher than that by glomeruli from normal rats. The higher rates of PGE2 production by glomeruli from diabetic rats are associated with higher levels of labeled free arach...
Saved in:
Published in | Diabetes (New York, N.Y.) Vol. 37; no. 4; pp. 429 - 435 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Alexandria, VA
American Diabetes Association
01.04.1988
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Prostaglandin E2 (PGE2) production by superfused glomeruli from rats made diabetic for 2 wk by streptozocin injection is twofold higher than that by glomeruli from normal rats. The higher rates of PGE2 production by glomeruli from diabetic rats are associated with higher levels of labeled free arachidonate in glomeruli prelabeled with [3H]arachidonate, both basally and after stimulation with Ca2+ ionophore A23187. The difference between release of labeled arachidonate from phospholipids of diabetic versus normal glomeruli is likely underestimated by measurements of arachidonate alone due to more rapid incorporation of released arachidonate into triacylglycerol of diabetic glomeruli. A23187 induced a fall in labeled phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol in glomeruli that had been prelabeled with [3H]arachidonate and also induced a reduction in the mass of these phospholipids. Consistent with the higher levels of labeled arachidonate, the reduction in both labeled phospholipids and phospholipid mass with A23187 was greater in glomeruli from diabetic than normal rats. Furthermore, the reduction in labeled phospholipids and phospholipid mass with A23187 was largely (62-80%) accounted for by a fall in phosphatidylcholine plus phosphatidylethanolamine in glomeruli from both normal and diabetic rats. These results suggest a primary role for phospholipase A2 in A23187 actions on glomerular arachidonate release in normal rats and for the higher levels of arachidonate found in glomeruli from diabetic rats. Nevertheless, A23187 also stimulated the production of inositol phosphates--a measure of cellular phospholipase C activity. |
---|---|
AbstractList | Prostaglandin E2 (PGE2) production by superfused glomeruli from rats made diabetic for 2 wk by streptozocin injection is twofold higher than that by glomeruli from normal rats. The higher rates of PGE2 production by glomeruli from diabetic rats are associated with higher levels of labeled free arachidonate in glomeruli prelabeled with [3H]arachidonate, both basally and after stimulation with Ca2+ ionophore A23187. The difference between release of labeled arachidonate from phospholipids of diabetic versus normal glomeruli is likely underestimated by measurements of arachidonate alone due to more rapid incorporation of released arachidonate into triacylglycerol of diabetic glomeruli. A23187 induced a fall in labeled phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol in glomeruli that had been prelabeled with [3H]arachidonate and also induced a reduction in the mass of these phospholipids. Consistent with the higher levels of labeled arachidonate, the reduction in both labeled phospholipids and phospholipid mass with A23187 was greater in glomeruli from diabetic than normal rats. Furthermore, the reduction in labeled phospholipids and phospholipid mass with A23187 was largely (62-80%) accounted for by a fall in phosphatidylcholine plus phosphatidylethanolamine in glomeruli from both normal and diabetic rats. These results suggest a primary role for phospholipase A2 in A23187 actions on glomerular arachidonate release in normal rats and for the higher levels of arachidonate found in glomeruli from diabetic rats. Nevertheless, A23187 also stimulated the production of inositol phosphates--a measure of cellular phospholipase C activity. Prostaglandin E2 (PGE2) production by superfused glomeruli from rats made diabetic for 2 wk by streptozocin injection is twofold higher than that by glomeruli from normal rats. The higher rates of PGE2 production by glomeruli from diabetic rats are associated with higher levels of labeled free arachidonate in glomeruli prelabeled with [3H]arachidonate, both basally and after stimulation with Ca2+ ionophore A23187. The difference between release of labeled arachidonate from phospholipids of diabetic versus normal glomeruli is likely underestimated by measurements of arachidonate alone due to more rapid incorporation of released arachidonate into triacylglycerol of diabetic glomeruli. A23187 induced a fall in labeled phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol in glomeruli that had been prelabeled with [3H]arachidonate and also induced a reduction in the mass of these phospholipids. Consistent with the higher levels of labeled arachidonate, the reduction in both labeled phospholipids and phospholipid mass with A23187 was greater in glomeruli from diabetic than normal rats. Furthermore, the reduction in labeled phospholipids and phospholipid mass with A23187 was largely (62–80%) accounted for by a fall in phosphatidylcholine plus phosphatidylethanolamine in glomeruli from both normal and diabetic rats. These results suggest a primary role for phospholipase A2 in A23187 actions on glomerular arachidonate release in normal rats and for the higher levels of arachidonate found in glomeruli from diabetic rats. Nevertheless, A23187 also stimulated the production of inositol phosphates—a measure of cellular phospholipase C activity. The increases in inositol phosphate production induced by A23187 were greater in glomeruli from diabetic than normal rats. However, the difference in inositol phosphate production between the two groups was abolished by indomethacin, suggesting that they were secondary to the enhanced prostanoid production by the diabetic glomeruli. Studies of phospholipase A2 and C activities in subcellular fractions of glomerular homogenates also supported a role for enhanced phospholipase A2 activity in the mediation of increased availability of arachidonate for prostaglandin synthesis in glomeruli from diabetic versus normal rats. Thus, phospholipase A2 activity was twofold higher in glomeruli from diabetic than normal rats, whereas phospholipase C activity was not different between the two groups. Treatment of diabetic rats with insulin prevented the rise in glomerular phospholipid A2 otherwise seen in these rats. |
Author | DERUBERTIS, F. R CRAVEN, P. A PATTERSON, M. C |
Author_xml | – sequence: 1 givenname: P. A surname: CRAVEN fullname: CRAVEN, P. A organization: Veterans administration medical cent., dep. medicine, Pittsburgh PA 15240, United States – sequence: 2 givenname: M. C surname: PATTERSON fullname: PATTERSON, M. C organization: Veterans administration medical cent., dep. medicine, Pittsburgh PA 15240, United States – sequence: 3 givenname: F. R surname: DERUBERTIS fullname: DERUBERTIS, F. R organization: Veterans administration medical cent., dep. medicine, Pittsburgh PA 15240, United States |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7818339$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/3132411$$D View this record in MEDLINE/PubMed |
BookMark | eNo9kUtr3TAQhUVJSW_SLrssaFGy860ediwvQ-gjECiUBLozY2l8rSJLriSn-Mf0v0aXXIIQs5iPw5lzLsiZDx4J-cjZXkjZfjEWhr1s9_W-Ft0bsuOd7Cop2t9nZMcYFxVvu_YduUjpD2Psurxzci65FDXnO_L_V3BIw0jRT-A1GgoR9GRN8JCRwhNYB4N1Nm80TzGsh4kuU0jlO7tAQnoj6AJ5-gcbtZ7OWPxkG_xR03odsTCGLjGkDAcH3hQobT5PmGyiw0YPLswYV2fpGMNMj-dgtppGyOk9eTuCS_jhNC_J47evD7c_qvuf3-9ub-4rLTqVK6kbMNgYhs0gSihcaGZAtGqUqsGCNJ1SoMx4zSSYjg8oUNeSa9A4Agd5Sa5edIvPvyum3M82aXTFL4Y19a0SjeA1K2D1AupyUIo49ku0M8St56w_1tEf_fdl1n2po_CfTsLrUKJ5pU_5l_3n0x6SBjfG0oFNr1iruJKyk8_Mf5nB |
CODEN | DIAEAZ |
CitedBy_id | crossref_primary_10_1038_ki_1992_151 crossref_primary_10_1007_BF02480649 crossref_primary_10_1016_j_rvsc_2011_12_017 crossref_primary_10_1038_ki_1992_152 crossref_primary_10_1016_0014_5793_91_81397_Q crossref_primary_10_1007_BF02552148 crossref_primary_10_1254_jphs_FPJ06010X crossref_primary_10_1016_0026_0495_93_90073_W crossref_primary_10_1152_ajpendo_1992_262_3_E301 crossref_primary_10_1007_BF00405080 crossref_primary_10_1046_j_1523_1755_1999_00590_x crossref_primary_10_1007_BF00400634 crossref_primary_10_1007_BF00573491 crossref_primary_10_1038_sj_ki_5001715 crossref_primary_10_1111_1440_1681_13478 crossref_primary_10_1016_S0272_6386_12_80439_2 crossref_primary_10_1371_journal_pone_0070029 crossref_primary_10_1038_ki_1993_183 crossref_primary_10_1177_1358836X0000500307 crossref_primary_10_1016_0925_4439_95_00116_6 crossref_primary_10_1007_BF00400475 crossref_primary_10_1074_jbc_M301175200 |
ContentType | Journal Article |
Copyright | 1988 INIST-CNRS |
Copyright_xml | – notice: 1988 INIST-CNRS |
DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
DOI | 10.2337/diab.37.4.429 |
DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE MEDLINE - Academic CrossRef |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine |
EISSN | 1939-327X |
EndPage | 435 |
ExternalDocumentID | 10_2337_diab_37_4_429 3132411 7818339 |
Genre | Research Support, U.S. Gov't, P.H.S Journal Article |
GrantInformation_xml | – fundername: NIDDK NIH HHS grantid: 5-R01-DK-34592 |
GroupedDBID | --- .55 .GJ .XZ 08P 08R 0R~ 18M 1CY 29F 2WC 354 3V. 4.4 53G 5GY 5RE 5RS 5VS 6PF 7RV 7X7 88E 88I 8AF 8AO 8C1 8F7 8FE 8FH 8FI 8FJ 8G5 8GL 8R4 8R5 AAKAS AAQQT AAUGY AAWTL AAYEP AAYJJ AAYOK ABOCM ABPTK ABUWG ACGFO ACGOD ACPRK ADBBV ADZCM AEGXH AENEX AERZD AFFNX AFHIN AFKRA AHMBA AI. AIAGR ALMA_UNASSIGNED_HOLDINGS AZQEC BAWUL BBNVY BCR BCU BEC BENPR BES BHPHI BKEYQ BKNYI BLC BPHCQ BTFSW BVXVI C1A CCPQU CS3 DIK DU5 DWQXO E3Z EBS EDB EJD EMOBN EX3 F5P FRP FYUFA GICCO GNUQQ GUQSH GX1 H13 HCIFZ HZ~ H~9 IAG IAO IEA IHR INH INR IOF IPO IQODW ITC J5H K-O K2M K9- KQ8 L7B LK8 M0R M1P M2O M2P M2Q M5~ M7P MVM N4W NAPCQ O5R O5S O9- OB3 OHH OK1 OVD P2P PCD PEA PQQKQ PROAC PSQYO Q2X RHF RHI RPM S0X SJFOW SJN SV3 TDI TEORI TR2 UKHRP VH1 VVN W8F WH7 WOQ WOW X7M XOL YFH YHG YOC YQJ ZA5 ZGI ZXP ZY1 ~KM AIZAD ALIPV CGR CUY CVF ECM EIF HMCUK NPM AAYXX CITATION 7X8 |
ID | FETCH-LOGICAL-c298t-3c5ade5d0e5b223312c0da278f385e2985988a8df603ad91be2ec431cacefa1a3 |
ISSN | 0012-1797 |
IngestDate | Sat Aug 17 00:33:08 EDT 2024 Fri Aug 23 03:32:40 EDT 2024 Sat Sep 28 08:32:46 EDT 2024 Sun Oct 29 17:07:37 EDT 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Keywords | Endocrinopathy Animal model Rat Arachidonic acid derivatives Diabetes mellitus Rodentia Prostaglandin E2 Biosynthesis Phospholipase A Kidney Renal glomerulus Vertebrata Mammalia |
Language | English |
License | CC BY 4.0 |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c298t-3c5ade5d0e5b223312c0da278f385e2985988a8df603ad91be2ec431cacefa1a3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 3132411 |
PQID | 78252140 |
PQPubID | 23479 |
PageCount | 7 |
ParticipantIDs | proquest_miscellaneous_78252140 crossref_primary_10_2337_diab_37_4_429 pubmed_primary_3132411 pascalfrancis_primary_7818339 |
PublicationCentury | 1900 |
PublicationDate | 1988-Apr |
PublicationDateYYYYMMDD | 1988-04-01 |
PublicationDate_xml | – month: 04 year: 1988 text: 1988-Apr |
PublicationDecade | 1980 |
PublicationPlace | Alexandria, VA |
PublicationPlace_xml | – name: Alexandria, VA – name: United States |
PublicationTitle | Diabetes (New York, N.Y.) |
PublicationTitleAlternate | Diabetes |
PublicationYear | 1988 |
Publisher | American Diabetes Association |
Publisher_xml | – name: American Diabetes Association |
SSID | ssj0006060 |
Score | 1.5352106 |
Snippet | Prostaglandin E2 (PGE2) production by superfused glomeruli from rats made diabetic for 2 wk by streptozocin injection is twofold higher than that by glomeruli... |
SourceID | proquest crossref pubmed pascalfrancis |
SourceType | Aggregation Database Index Database |
StartPage | 429 |
SubjectTerms | Animals Arachidonic Acids - metabolism Biological and medical sciences Calcimycin - pharmacology Diabetes Mellitus, Experimental - metabolism Diabetes. Impaired glucose tolerance Diglycerides - metabolism Dinoprostone Endocrine pancreas. Apud cells (diseases) Endocrinopathies Female Inositol Phosphates - metabolism Kidney Glomerulus - metabolism Medical sciences Phospholipases - metabolism Phospholipases A - metabolism Phospholipases A2 Phospholipids - metabolism Prostaglandins E - biosynthesis Rats Tissue Distribution Triglycerides - metabolism Type C Phospholipases - metabolism |
Title | Role of enhanced arachidonate availability through phospholipase A2 pathway in mediation of increased prostaglandin synthesis by glomeruli from diabetic rats |
URI | https://www.ncbi.nlm.nih.gov/pubmed/3132411 https://search.proquest.com/docview/78252140 |
Volume | 37 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1db9MwFLXKEGgSQnxNFBj4AfGCUlLHaZLHaWUaoKFp2qS9RU5sr5G6pFobUPkL_Ab-K_faTppUTAKkNq0S10l7Ttxj-95jQt4mKtaaK5xkldBBSZLcEyCLvTzxZZj5kywKMXf45Ovk-IJ_vgwvB4OfnailepWN8h9_zCv5H1RhH-CKWbL_gGxbKeyA94AvbAFh2P4VxmcuNFCVMzuRj-bLs0LiiLh6L76JYm5tuNftejyLWbWE57xYCLQkYWisOvsuTPafySJpFGRRop5cKrQRqEBCmrU-oNByXYJmRBsTEK5X8-pa3dRzl6ZiB3LRFFpYg6hG9k47I7zba_90xiIOcS0k0wyebsZYT60DqIvy34zqTtVZbaLCDROPXOSjtBl9AOEm7sU1ymOGLqlRt1G2TjCOfLzTwnJ7UdstPwuMdwB-z1EQjfhoqxwAt7g2NECvSu4a-L7Ttjtyh9xlURJifOj005f2bx16ejafyV2tNWzF837onXWX3HMV9aTOA4AV7jptl0u5vT9jdM35I_LQdUjogWXXYzJQ5RNy_8SFXDwlv5BktNK0IRntkox2SUYdyWiPZPSAUUcyWpS0JRnW2ZKM9khGW5LRbE1bklEkGW1IRpFkz8jF0cfzw2PPrenh5SyJV16Qh0KqUPoqzECZBmOW-1KwKNZBHCooEgJDRCz1xA-ETMaZYioHkZuLXGkxFsEe2SmrUj0nVMkkg4diOoq5hp4OVJtpHqHDpwBlPCTvmp8_XVjrlhS6vIhYipeawitPAbEh2e-B05aOQM0GARx_04CVQuOLM2qiVFW9hAIM5C_3h2TPYth-1FHgxW0HXpLdzb3wiuysbmq1D_J2lb02tPsNSs-rSw |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | Flying Publisher |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Role+of+enhanced+arachidonate+availability+through+phospholipase+A2+pathway+in+mediation+of+increased+prostaglandin+synthesis+by+glomeruli+from+diabetic+rats&rft.jtitle=Diabetes+%28New+York%2C+N.Y.%29&rft.au=Craven%2C+P+A&rft.au=Patterson%2C+M+C&rft.au=DeRubertis%2C+F+R&rft.date=1988-04-01&rft.issn=0012-1797&rft.volume=37&rft.issue=4&rft.spage=429&rft_id=info:doi/10.2337%2Fdiab.37.4.429&rft_id=info%3Apmid%2F3132411&rft.externalDocID=3132411 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0012-1797&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0012-1797&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0012-1797&client=summon |