Gaultherin production from gandapura (Gandapura fragantissima) using photo extractor-UV machine

• Published in: Materials today : proceedings Vol. 63; pp. S183 - S187
• Main Authors: Sutrisno, Yulianto, Mohamad Endy, Ariwibowo, Didik, Nyamiati, Retno Dwi, Rahma, Maudina Yunia, Ainurrofiq, Muhammad
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 2022
• Subjects: Gandapura; Gaultherin; Photo extractor-UV machine; Gaultherin; Photo extractor-UV machine; Gandapura
• ISSN: 2214-7853; 2214-7853
• DOI: 10.1016/j.matpr.2022.02.213

Gandapura extraction process using ethanol as a polar compound has two functions, namely: deactivating the gaultherase enzyme and extracting the active compound gaultherin. This study examines the effect of extraction using UV from the concentration of ethanol in the extraction process of gandapura leaves into gaultherin on the extraction time, with a ratio of solvent and feed of 5:1, the rotational speed of the stirrer and the rotational speed of the chopper blades are 75 and 125 rpm, respectively. Drying agent is added according to the experimental variables; the independent variables includes ethanol concentrations of 85, 90, and 96%, while the extraction times are 10, 30 and 50 min, respectively. Gaultherin concentration was obtained from HPLC Alliance 2695 (Waters) analysis with Photodiode Array Detector 2996 (Waters) at a wavelength of 254 nm. The effect of UV photo extraction on the acquisition of gaultherin compounds has succeeded in deactivating the gaultherase enzyme and opening when it is penetrated with ethanol. In this study, the peak of the dominant gaultherin compound was found at the retention time of 5.8 min, and the highest area was at 90% ethanol concentration variable with an extraction time of 50 min; the area was 22.783% of the total gaultherin area.