Determination of serum d-lactic and l-lactic acids in normal subjects and diabetic patients by column-switching HPLC with pre-column fluorescence derivatization

• Published in: Analytical and bioanalytical chemistry Vol. 377; no. 5; pp. 886 - 891
• Main Authors: Hasegawa, Hisanori, Fukushima, Takeshi, Lee, Jen-Ai, Tsukamoto, Kazuhisa, Moriya, Kyoji, Ono, Yoshikazu, Imai, Kazuhiro
• Format: Journal Article
• Language: English
• Published: Germany 01.11.2003
• Subjects: Adult; Aged; Chromatography, High Pressure Liquid - methods; Diabetes Mellitus - blood; Fluorescent Dyes - chemistry; Humans; Lactate Dehydrogenases - blood; Lactate Dehydrogenases - chemistry; Lactic Acid - blood; Lactic Acid - chemistry; Middle Aged; Oxadiazoles - chemistry; Piperazines - chemistry; Spectrometry, Fluorescence; Stereoisomerism; Time Factors
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-003-2108-6

d-Lactic and l-lactic acids were simultaneously determined by means of a column-switching high-performance liquid chromatography (HPLC) with fluorescence detection. As a fluorescence reagent, 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) was employed for the fluorescence derivatization of lacti...