Preparation of cinnamoyl-CoA and analysis of the enzyme activity of recombinant CCR protein from Populus tomentosa
Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44), which catalyzes the reduction of cinnamoyl-CoA esters to their respective cinnamaldehydes, is considered as a key enzyme in lignin formation. The substrates of CCR, cinnamoyl-CoA esters, are products of 4-Coumarate-CoA ligase (4CL, EC 6.2.1.12), which is a...
Saved in:
Published in | Journal of plant biochemistry and biotechnology Vol. 23; no. 4; pp. 348 - 357 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
India
Springer-Verlag
01.10.2014
Springer India |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44), which catalyzes the reduction of cinnamoyl-CoA esters to their respective cinnamaldehydes, is considered as a key enzyme in lignin formation. The substrates of CCR, cinnamoyl-CoA esters, are products of 4-Coumarate-CoA ligase (4CL, EC 6.2.1.12), which is an enzyme upstream of CCR. The PtCCR and Pt4CL were isolated from Populus tomentosa and expressed in E. coli. Results showed that 4CL can catalyze the conversion of hydroxycinnamic acids to cinnamoyl-CoA esters, with high efficiency. The purification of esters using SPE cartridges suggested that 40 % methanol with 0.1 M of acetic acid was the optimal elution buffer for cinnamoyl-CoA esters. The optimization of prokaryotic expression demonstrated that the best expression conditions for recombinant PtCCR was 6 h of 0.4 mM IPTG induction at 37 °C. PtCCR enzyme assay illustrated that the recombinant protein can catalyze the reduction of cinnamoyl-CoA esters. Kinetics analysis showed that feruloyl-CoA has higher affinity to PtCCR with faster reaction speed (Vmax), indicating that feruloyl-CoA was the most favorable substrate for PtCCR catalysis. The recombinant protein was expressed in E. coli, purified through affinity column chromatography, and characterized by SDS-PAGE. SPE cartridges were used to purify the ester products of the Pt4CL reaction. HPLC-MS was used to analyze the structure of esters and evaluate their purity or quantity. Furthermore, the enzyme activity of recombinant CCR to feruloyl-CoA at different pHs indicated that compartmentalization may be an important factor in lignin monomer formation. |
---|---|
AbstractList | Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44), which catalyzes the reduction of cinnamoyl-CoA esters to their respective cinnamaldehydes, is considered as a key enzyme in lignin formation. The substrates of CCR, cinnamoyl-CoA esters, are products of 4-Coumarate-CoA ligase (4CL, EC 6.2.1.12), which is an enzyme upstream of CCR. The PtCCR and Pt4CL were isolated from Populus tomentosa and expressed in
E. coli.
Results showed that 4CL can catalyze the conversion of hydroxycinnamic acids to cinnamoyl-CoA esters, with high efficiency. The purification of esters using SPE cartridges suggested that 40 % methanol with 0.1 M of acetic acid was the optimal elution buffer for cinnamoyl-CoA esters. The optimization of prokaryotic expression demonstrated that the best expression conditions for recombinant PtCCR was 6 h of 0.4 mM IPTG induction at 37 °C. PtCCR enzyme assay illustrated that the recombinant protein can catalyze the reduction of cinnamoyl-CoA esters. Kinetics analysis showed that feruloyl-CoA has higher affinity to PtCCR with faster reaction speed (Vmax), indicating that feruloyl-CoA was the most favorable substrate for PtCCR catalysis. The recombinant protein was expressed in E. coli, purified through affinity column chromatography, and characterized by SDS-PAGE. SPE cartridges were used to purify the ester products of the Pt4CL reaction. HPLC-MS was used to analyze the structure of esters and evaluate their purity or quantity. Furthermore, the enzyme activity of recombinant CCR to feruloyl-CoA at different pHs indicated that compartmentalization may be an important factor in lignin monomer formation. Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44), which catalyzes the reduction of cinnamoyl-CoA esters to their respective cinnamaldehydes, is considered as a key enzyme in lignin formation. The substrates of CCR, cinnamoyl-CoA esters, are products of 4-Coumarate-CoA ligase (4CL, EC 6.2.1.12), which is an enzyme upstream of CCR. The PtCCR and Pt4CL were isolated from Populus tomentosa and expressed in E. coli. Results showed that 4CL can catalyze the conversion of hydroxycinnamic acids to cinnamoyl-CoA esters, with high efficiency. The purification of esters using SPE cartridges suggested that 40 % methanol with 0.1 M of acetic acid was the optimal elution buffer for cinnamoyl-CoA esters. The optimization of prokaryotic expression demonstrated that the best expression conditions for recombinant PtCCR was 6 h of 0.4 mM IPTG induction at 37 degree C. PtCCR enzyme assay illustrated that the recombinant protein can catalyze the reduction of cinnamoyl-CoA esters. Kinetics analysis showed that feruloyl-CoA has higher affinity to PtCCR with faster reaction speed (Vmax), indicating that feruloyl-CoA was the most favorable substrate for PtCCR catalysis. The recombinant protein was expressed in E. coli, purified through affinity column chromatography, and characterized by SDS-PAGE. SPE cartridges were used to purify the ester products of the Pt4CL reaction. HPLC-MS was used to analyze the structure of esters and evaluate their purity or quantity. Furthermore, the enzyme activity of recombinant CCR to feruloyl-CoA at different pHs indicated that compartmentalization may be an important factor in lignin monomer formation. Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44), which catalyzes the reduction of cinnamoyl-CoA esters to their respective cinnamaldehydes, is considered as a key enzyme in lignin formation. The substrates of CCR, cinnamoyl-CoA esters, are products of 4-Coumarate-CoA ligase (4CL, EC 6.2.1.12), which is an enzyme upstream of CCR. The PtCCR and Pt4CL were isolated from Populus tomentosa and expressed in E. coli. Results showed that 4CL can catalyze the conversion of hydroxycinnamic acids to cinnamoyl-CoA esters, with high efficiency. The purification of esters using SPE cartridges suggested that 40 % methanol with 0.1 M of acetic acid was the optimal elution buffer for cinnamoyl-CoA esters. The optimization of prokaryotic expression demonstrated that the best expression conditions for recombinant PtCCR was 6 h of 0.4 mM IPTG induction at 37 °C. PtCCR enzyme assay illustrated that the recombinant protein can catalyze the reduction of cinnamoyl-CoA esters. Kinetics analysis showed that feruloyl-CoA has higher affinity to PtCCR with faster reaction speed (Vmax), indicating that feruloyl-CoA was the most favorable substrate for PtCCR catalysis. The recombinant protein was expressed in E. coli, purified through affinity column chromatography, and characterized by SDS-PAGE. SPE cartridges were used to purify the ester products of the Pt4CL reaction. HPLC-MS was used to analyze the structure of esters and evaluate their purity or quantity. Furthermore, the enzyme activity of recombinant CCR to feruloyl-CoA at different pHs indicated that compartmentalization may be an important factor in lignin monomer formation. |
Author | Gai, Ying Jiang, Xiangning Liu, Shaoli Han, Botao |
Author_xml | – sequence: 1 fullname: Han, Botao – sequence: 2 fullname: Liu, Shaoli – sequence: 3 fullname: Gai, Ying – sequence: 4 fullname: Jiang, Xiangning |
BookMark | eNp9kM1q3TAQhUVIIb8PkFW1zEbtSLZk32UwSVoINDTJWoxlKVWwpVtJDrhPH9-66y6GGc6cMwzfGTkOMVhCrjh84QDN18wrqQQDXjEQvGXqiJzCrqkZF408_jtz1rScn5CznN8AatlAfUrSY7J7TFh8DDQ6anwIOMVlZF28oRiGtXBcss-HbfllqQ1_lslSNMW_-7Ic5GRNnHofMBTadT_pPsVifaAuxYk-xv08zpmWONlQYsYL8snhmO3lv35OXu5un7tv7OHH_ffu5oEZsWsKazliq2wvhqoBWQmDXAyuh1VoUSqjpAJeD4hGONyBU25X9cid6PsW3SCqc3K93V3f-T3bXPTks7HjiMHGOWuuFNRQVy2sVr5ZTYo5J-v0PvkJ06I56ANfvfHVK1994KvVmhFbJq_e8GqTfotzWmHl_4Y-byGHUeNr8lm_PAngEgCkkrWsPgDXF4rS |
Cites_doi | 10.1111/j.1432-1033.1981.tb05584.x 10.1073/pnas.96.16.8955 10.1073/pnas.95.22.12803 10.1093/pcp/pci120 10.1515/BC.2005.065 10.1016/S0168-9452(00)00417-9 10.1007/s11248-007-9113-z 10.1006/abio.2001.5574 10.1074/jbc.M109.036673 10.1016/S0168-9452(99)00041-2 10.1111/j.1469-8137.2009.03018.x 10.1073/pnas.0509875103 10.1078/0176-1617-01074 10.1105/tpc.13.7.1567 10.1016/j.pbi.2008.03.005 10.1105/tpc.6.10.1427 10.1006/abio.2000.4760 10.1016/S0735-2689(98)00360-8 10.1007/BF03182856 10.1111/j.1432-1033.1984.tb08002.x 10.1023/A:1006060101866 10.1016/S0031-9422(96)00594-8 10.1105/tpc.107.054148 10.1074/jbc.275.9.6537 10.1016/0003-9861(77)90347-2 10.1016/S0176-1617(00)80002-4 10.1023/A:1006071708728 10.1080/10409230391036757 10.1021/bi970025j 10.1078/0176-1617-00719 10.1105/tpc.109.072652 10.1007/s12374-009-9062-6 10.1046/j.1432-1327.2001.02255.x 10.1002/cbf.1131 10.1073/pnas.90.9.4102 10.1105/tpc.7.7.1001 10.1016/S0031-9422(01)00053-X 10.1007/s10265-004-0159-1 10.1073/pnas.96.18.10045 10.1023/A:1006244325250 10.1105/tpc.109.072827 10.1093/jxb/erm064 10.1016/j.plaphy.2005.06.003 10.1104/pp.111.178301 10.1046/j.1365-313X.1997.11030429.x 10.1105/tpc.020297 10.1111/j.1432-1033.1975.tb02418.x 10.1104/pp.119.1.49 10.1093/pcp/pce060 10.1016/0031-9422(93)85102-W 10.1111/j.1432-1033.1976.tb10370.x 10.1515/znc-1975-5-609 10.1007/s00425-003-0987-6 10.1104/pp.106.2.625 10.1007/s11033-012-2312-6 10.1016/S0021-9258(19)49654-3 |
ContentType | Journal Article |
Copyright | Society for Plant Biochemistry and Biotechnology 2013 |
Copyright_xml | – notice: Society for Plant Biochemistry and Biotechnology 2013 |
DBID | FBQ AAYXX CITATION 7QO 8FD FR3 P64 |
DOI | 10.1007/s13562-013-0218-6 |
DatabaseName | AGRIS CrossRef Biotechnology Research Abstracts Technology Research Database Engineering Research Database Biotechnology and BioEngineering Abstracts |
DatabaseTitle | CrossRef Engineering Research Database Biotechnology Research Abstracts Technology Research Database Biotechnology and BioEngineering Abstracts |
DatabaseTitleList | Engineering Research Database |
Database_xml | – sequence: 1 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
DeliveryMethod | fulltext_linktorsrc |
EISSN | 0974-1275 |
EndPage | 357 |
ExternalDocumentID | 10_1007_s13562_013_0218_6 US201500056545 |
GroupedDBID | -EM 06D 0R~ 0VY 203 29~ 2KG 2KM 2VQ 30V 4.4 406 408 5GY 67N 96X AAAVM AABYN AAFGU AAHNG AAIAL AAJKR AANXM AANZL AARHV AARTL AATNV AATVU AAUYE AAWCG AAYFA AAYIU AAYQN AAYTO AAZMS ABDZT ABECU ABELW ABFGW ABFTV ABJNI ABJOX ABKAS ABKCH ABMQK ABPLI ABQBU ABSXP ABTEG ABTHY ABTKH ABTMW ABULA ABXPI ACBMV ACBRV ACBYP ACGFO ACGFS ACHSB ACIGE ACIPQ ACKNC ACMDZ ACMLO ACOKC ACPRK ACREN ACTTH ACVWB ACWMK ADHHG ADHIR ADINQ ADKNI ADKPE ADMDM ADOAH ADOXG ADRFC ADTPH ADURQ ADYFF ADYOE ADYPR ADZKW AEBTG AEEQQ AEFTE AEGNC AEJHL AEJRE AENEX AEOHA AEPYU AESKC AESTI AETCA AEVLU AEVTX AEXYK AFLOW AFNRJ AFQWF AFRAH AFWTZ AFYQB AFZKB AGAYW AGDGC AGGBP AGJBK AGMZJ AGQMX AGWZB AGYKE AHAVH AHBYD AHKAY AHSBF AHYZX AIAKS AIIXL AILAN AIMYW AITGF AJBLW AJDOV AJRNO AJZVZ AKMHD AKQUC ALFXC ALMA_UNASSIGNED_HOLDINGS AMKLP AMTXH AMXSW AMYLF AMYQR ANMIH AOCGG AOSHJ ASPBG AVWKF AXYYD AZFZN BGNMA CSCUP DDRTE DNIVK DPUIP DYU EBLON EBS EIOEI EJD ESBYG FBQ FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FYJPI GGCAI GGRSB GJIRD GQ6 GQ7 HF~ HMJXF HRMNR HZ~ I0C IKXTQ IWAJR IXD J-C JBSCW JZLTJ KOV LLZTM M4Y NPVJJ NQJWS NU0 O9- O93 O9I O9J OZF P2P PT4 R9I RHV RLLFE RSV S1Z S27 S3A S3B SBL SBY SCLPG SHX SISQX SNE SNPRN SNX SOHCF SOJ SPISZ SRMVM SSLCW SSXJD STPWE T13 TSG U2A U9L UG4 UOJIU UTJUX UZXMN VC2 VFIZW W48 Z7U ZMTXR ZOVNA ~A9 AACDK AAEOY AAEWM AAHBH AAJBT AAQLM AASML AAYXX ABAKF ACAOD ACDTI ACZOJ AEFQL AEMSY AFBBN AGQEE AGRTI AIGIU CITATION H13 ROL SJYHP 7QO 8FD FR3 P64 |
ID | FETCH-LOGICAL-c297t-81aa86eb2d370532ca12dfb0b2d8a56c656014daac2fa90f6f93ba1f2bb8afd23 |
IEDL.DBID | AGYKE |
ISSN | 0971-7811 |
IngestDate | Fri Aug 16 21:12:41 EDT 2024 Thu Sep 12 19:01:26 EDT 2024 Sat Dec 16 11:59:56 EST 2023 Wed Dec 27 19:14:28 EST 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Keywords | SPE Cinnamoyl-CoA reductase Cinnamoyl-CoA esters HPLC-MS |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c297t-81aa86eb2d370532ca12dfb0b2d8a56c656014daac2fa90f6f93ba1f2bb8afd23 |
Notes | http://dx.doi.org/10.1007/s13562-013-0218-6 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PQID | 1660404380 |
PQPubID | 23462 |
PageCount | 10 |
ParticipantIDs | proquest_miscellaneous_1660404380 crossref_primary_10_1007_s13562_013_0218_6 springer_journals_10_1007_s13562_013_0218_6 fao_agris_US201500056545 |
PublicationCentury | 2000 |
PublicationDate | 2014-10-01 |
PublicationDateYYYYMMDD | 2014-10-01 |
PublicationDate_xml | – month: 10 year: 2014 text: 2014-10-01 day: 01 |
PublicationDecade | 2010 |
PublicationPlace | India |
PublicationPlace_xml | – name: India |
PublicationTitle | Journal of plant biochemistry and biotechnology |
PublicationTitleAbbrev | J. Plant Biochem. Biotechnol |
PublicationYear | 2014 |
Publisher | Springer-Verlag Springer India |
Publisher_xml | – name: Springer-Verlag – name: Springer India |
References | Goujon, Ferret, Mila, Pollet, Ruel, Burlat, Joseleau, Barriere, Lapierre, Jouanin (CR13) 2003; 217 Hu, Gai, Yin, Wang, Feng, Feng, Li, Jiang, Wang (CR18) 2010; 22 Stockigt, Zenk (CR45) 1975; 30 Koopmann, Logemann, Hahlbrock (CR22) 1999; 119 McInnes, Lidgett, Lynch, Huxley, Jones, Mahoney, Spangenberg (CR35) 2002; 159 Meng, Campbell (CR37) 1998; 38 Teutsch, Hasenfratz, Lesot, Stoltz, Garnier, Jeltsch, Durst, Werck-Reichhart (CR46) 1993; 90 Ye, Kneusel, Matern, Varner (CR56) 1994; 6 Escamilla-Trevino, Shen, Uppalapati, Ray, Tang, Hernandez, Yin, Xu, Dixon (CR11) 2010; 185 Li, Osakabe, Joshi, Chiang (CR28) 1999; 40 Hoffmann, Besseau, Geoffroy, Ritzenthaler, Meyer, Lapierre, Pollet, Legrand (CR17) 2004; 16 Tu, Rochfort, Liu, Ran, Griffith, Badenhorst, Louie, Bowman, Smith, Noel, Mouradov, Spangenberg (CR48) 2010; 22 Yong, Wei, Tianyun, Yuxian (CR57) 2003; 48 Gui, Shen, Li (CR15) 2011; 157 Baltas, Lapeyre, Bedos-Belval, Maturano, Saint-Aguet, Roussel, Duran, Grima-Pettenati (CR3) 2005; 43 Pichon, Courbou, Beckert, Boudet, Grima-Pettenati (CR41) 1998; 38 Vanholme, Morreel, Ralph, Boerjan (CR49) 2008; 11 Abe, Takahashi, Morita, Noguchi (CR1) 2001; 268 Li, Cheng, Leshkevich, Umezawa, Harding, Chiang (CR30) 2001; 13 De Cooman, Everaert, Faché, Casteele, Van Sumere (CR9) 1993; 33 Meng, Campbell (CR36) 1997; 44 Obel, Scheller (CR38) 2000; 286 Ma (CR33) 2007; 58 CR40 Beuerle, Pichersky (CR7) 2002; 302 Thoden, Hegeman, Wesenberg, Chapeau, Frey, Holden (CR47) 1997; 36 Gout, Bligny, Douce (CR14) 1992; 267 Ralph, Hatfield, Piquemal, Yahiaoui, Pean, Lapierre, Boudet (CR42) 1998; 95 Yabuya, Yamaguchi, Fukui, Katoh, Imayama, Ino (CR55) 2001; 160 Kawasaki, Koita, Nakatsubo, Hasegawa, Wakabayashi, Takahashi, Urnemura, Urnezawa, Shimamoto (CR20) 2006; 103 Bernards, Susag, Bedgar, Anterola, Lewis (CR6) 2000; 157 Back, Jang, Lee, Schmidt, Strack, Kim (CR2) 2001; 42 Luderitz, Grisebach (CR32) 1981; 119 Li, Cheng, Lu, Nakatsubo, Umezawa, Chiang (CR31) 2005; 46 Wadenback, von Arnold, Egertsdotter, Walter, Grima-Pettenati, Goffner, Gellerstedt, Gullion, Clapham (CR50) 2008; 17 Lauvergeat, Lacomme, Lacombe, Lasserre, Roby, Grima-Pettenati (CR25) 2001; 57 Leple, Dauwe, Morreel, Storme, Lapierre, Pollet, Naumann, Kang, Kim, Ruel, Lefebvre, Joseleau, Grima-Pettenati, De Rycke, Andersson-Gunneras, Erban, Fehrle, Petit-Conil, Kopka, Polle, Messens, Sundberg, Mansfield, Ralph, Pilate, Boerjan (CR27) 2007; 19 Baucher, Halpin, Petit-Conil, Boerjan (CR5) 2003; 38 Whetten, Sederoff (CR53) 1995; 7 El-Batal (CR10) 2002; 51 Selman-Housein, MaA, Hernández, Civardi, Miranda, Rigau, Puigdomènech (CR44) 1999; 143 Knobloch, Hahlbrock (CR21) 1977; 184 Ma, Tian (CR34) 2005; 386 Hamada, Nishida, Yamauchi, Fukushima, Kondo, Tsutsumi (CR16) 2004; 117 Sarni, Grand, Boudet (CR43) 1984; 139 Wengenmayer, Ebel, Grisebach (CR52) 1976; 65 Baucher, Monties, Van Montagu, Boerjan (CR4) 1998; 17 Goffner, Campbell, Campargue, Clastre, Borderies, Boudet, Boudet (CR12) 1994; 106 Lacombe, Hawkins, Van Doorsselaere, Piquemal, Goffner, Poeydomenge, Boudet, Grima-Pettenati (CR23) 1997; 11 Wyrambik, Grisebach (CR54) 1975; 59 Lazar (CR26) 2005; 23 Larsen (CR24) 2004; 161 Osakabe, Tsao, Li, Popko, Umezawa, Carraway, Smeltzer, Joshi, Chiang (CR39) 1999; 96 Humphreys, Hemm, Chapple (CR19) 1999; 96 Li, Popko, Umezawa, Chiang (CR29) 2000; 275 Wang, Bai, Hai, Xiang-Ning (CR51) 2009; 52 Bhuiya, Liu (CR8) 2010; 285 H Meng (218_CR37) 1998; 38 T Lazar (218_CR26) 2005; 23 K Larsen (218_CR24) 2004; 161 R Vanholme (218_CR49) 2008; 11 L Li (218_CR28) 1999; 40 K Osakabe (218_CR39) 1999; 96 Y Tu (218_CR48) 2010; 22 F Sarni (218_CR43) 1984; 139 M Pichon (218_CR41) 1998; 38 L Li (218_CR31) 2005; 46 T Yabuya (218_CR55) 2001; 160 R Whetten (218_CR53) 1995; 7 T Luderitz (218_CR32) 1981; 119 E Gout (218_CR14) 1992; 267 QH Ma (218_CR33) 2007; 58 K Hamada (218_CR16) 2004; 117 M Baltas (218_CR3) 2005; 43 T Kawasaki (218_CR20) 2006; 103 MW Bhuiya (218_CR8) 2010; 285 218_CR40 K-H Knobloch (218_CR21) 1977; 184 J Ralph (218_CR42) 1998; 95 J Wadenback (218_CR50) 2008; 17 M Baucher (218_CR5) 2003; 38 K Back (218_CR2) 2001; 42 L Cooman De (218_CR9) 1993; 33 D Goffner (218_CR12) 1994; 106 T Beuerle (218_CR7) 2002; 302 H Wengenmayer (218_CR52) 1976; 65 Y Hu (218_CR18) 2010; 22 E Koopmann (218_CR22) 1999; 119 JB Thoden (218_CR47) 1997; 36 JM Humphreys (218_CR19) 1999; 96 QH Ma (218_CR34) 2005; 386 L Hoffmann (218_CR17) 2004; 16 V Lauvergeat (218_CR25) 2001; 57 T Goujon (218_CR13) 2003; 217 J Stockigt (218_CR45) 1975; 30 M Baucher (218_CR4) 1998; 17 N Obel (218_CR38) 2000; 286 D-D Wang (218_CR51) 2009; 52 AI El-Batal (218_CR10) 2002; 51 MA Bernards (218_CR6) 2000; 157 L Li (218_CR29) 2000; 275 HG Teutsch (218_CR46) 1993; 90 LL Escamilla-Trevino (218_CR11) 2010; 185 L Li (218_CR30) 2001; 13 ZH Ye (218_CR56) 1994; 6 G Selman-Housein (218_CR44) 1999; 143 D Wyrambik (218_CR54) 1975; 59 E Lacombe (218_CR23) 1997; 11 BAI Yong (218_CR57) 2003; 48 H Meng (218_CR36) 1997; 44 JC Leple (218_CR27) 2007; 19 I Abe (218_CR1) 2001; 268 J Gui (218_CR15) 2011; 157 R McInnes (218_CR35) 2002; 159 |
References_xml | – volume: 119 start-page: 115 issue: 1 year: 1981 end-page: 124 ident: CR32 article-title: Enzymic synthesis of lignin precursors. Comparison of cinnamoyl-CoA reductase and cinnamyl alcohol:NADP + dehydrogenase from spruce (Picea abies L.) and soybean (Glycine max L.) publication-title: Eur J Biochem doi: 10.1111/j.1432-1033.1981.tb05584.x contributor: fullname: Grisebach – volume: 96 start-page: 8955 issue: 16 year: 1999 end-page: 8960 ident: CR39 article-title: Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.96.16.8955 contributor: fullname: Chiang – volume: 95 start-page: 12803 issue: 22 year: 1998 end-page: 12808 ident: CR42 article-title: NMR characterization of altered lignins extracted from tobacco plants down-regulated for lignification enzymes cinnamylalcohol dehydrogenase and cinnamoyl-CoA reductase publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.95.22.12803 contributor: fullname: Boudet – volume: 46 start-page: 1073 issue: 7 year: 2005 end-page: 1082 ident: CR31 article-title: Clarification of cinnamoyl co-enzyme A reductase catalysis in monolignol biosynthesis of Aspen publication-title: Plant Cell Physiol doi: 10.1093/pcp/pci120 contributor: fullname: Chiang – volume: 386 start-page: 553 issue: 6 year: 2005 end-page: 560 ident: CR34 article-title: Biochemical characterization of a cinnamoyl-CoA reductase from wheat publication-title: Biol Chem doi: 10.1515/BC.2005.065 contributor: fullname: Tian – volume: 160 start-page: 499 issue: 3 year: 2001 end-page: 503 ident: CR55 article-title: Characterization of anthocyanin p-coumaroyltransferase in flowers of Iris ensata publication-title: Plant Sci doi: 10.1016/S0168-9452(00)00417-9 contributor: fullname: Ino – volume: 267 start-page: 13903 issue: 20 year: 1992 end-page: 13909 ident: CR14 article-title: Regulation of intracellular pH values in higher plant cells. Carbon-13 and phosphorus-31 nuclear magnetic resonance studies publication-title: J Biol Chem contributor: fullname: Douce – volume: 17 start-page: 379 issue: 3 year: 2008 end-page: 392 ident: CR50 article-title: Lignin biosynthesis in transgenic Norway spruce plants harboring an antisense construct for cinnamoyl CoA reductase (CCR) publication-title: Transgenic Res doi: 10.1007/s11248-007-9113-z contributor: fullname: Clapham – volume: 106 start-page: 625 issue: 2 year: 1994 end-page: 632 ident: CR12 article-title: Purification and characterization of cinnamoyl-coenzyme A:NADP oxidoreductase in eucalyptus gunnii publication-title: Plant Physiol contributor: fullname: Boudet – volume: 302 start-page: 305 issue: 2 year: 2002 end-page: 312 ident: CR7 article-title: Enzymatic synthesis and purification of aromatic coenzyme a esters publication-title: Anal Biochem doi: 10.1006/abio.2001.5574 contributor: fullname: Pichersky – volume: 30 start-page: 352 issue: 3 year: 1975 end-page: 358 ident: CR45 article-title: Chemical syntheses and properties of hydroxycinnamoyl-coenzyme A derivatives publication-title: Z Naturforsch C Biosci contributor: fullname: Zenk – volume: 285 start-page: 277 issue: 1 year: 2010 end-page: 285 ident: CR8 article-title: Engineering monolignol 4-O-methyltransferases to modulate lignin biosynthesis publication-title: J Biol Chem doi: 10.1074/jbc.M109.036673 contributor: fullname: Liu – volume: 143 start-page: 163 issue: 2 year: 1999 end-page: 171 ident: CR44 article-title: Molecular cloning of cDNAs coding for three sugarcane enzymes involved in lignification publication-title: Plant Sci doi: 10.1016/S0168-9452(99)00041-2 contributor: fullname: Puigdomènech – volume: 185 start-page: 143 issue: 1 year: 2010 end-page: 155 ident: CR11 article-title: Switchgrass (Panicum virgatum) possesses a divergent family of cinnamoyl CoA reductases with distinct biochemical properties publication-title: New Phytol doi: 10.1111/j.1469-8137.2009.03018.x contributor: fullname: Dixon – volume: 103 start-page: 230 issue: 1 year: 2006 end-page: 235 ident: CR20 article-title: Cinnamoyl-CoA reductase, a key enzyme in lignin biosynthesis, is an effector of small GTPase Rac in defense signaling in rice publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0509875103 contributor: fullname: Shimamoto – volume: 161 start-page: 105 issue: 1 year: 2004 end-page: 112 ident: CR24 article-title: Molecular cloning and characterization of cDNAs encoding cinnamoyl CoA reductase (CCR) from barley (Hordeum vulgare) and potato (Solanum tuberosum) publication-title: J Plant Physiol doi: 10.1078/0176-1617-01074 contributor: fullname: Larsen – volume: 51 start-page: 139 issue: 2 year: 2002 end-page: 152 ident: CR10 article-title: Optimization of reaction conditions and stabilization of phenylalanine ammonia lyase-containing Rhodotorula glutinis cells during bioconversion of trans-cinnamic acid to L-phenylalanine publication-title: Acta Microbiol Pol contributor: fullname: El-Batal – volume: 13 start-page: 1567 issue: 7 year: 2001 end-page: 1586 ident: CR30 article-title: The last step of syringyl monolignol biosynthesis in angiosperms is regulated by a novel gene encoding sinapyl alcohol dehydrogenase publication-title: Plant Cell doi: 10.1105/tpc.13.7.1567 contributor: fullname: Chiang – volume: 11 start-page: 278 issue: 3 year: 2008 end-page: 285 ident: CR49 article-title: Lignin engineering publication-title: Curr Opin Plant Biol doi: 10.1016/j.pbi.2008.03.005 contributor: fullname: Boerjan – volume: 6 start-page: 1427 issue: 10 year: 1994 end-page: 1439 ident: CR56 article-title: An alternative methylation pathway in lignin biosynthesis in Zinnia publication-title: Plant Cell doi: 10.1105/tpc.6.10.1427 contributor: fullname: Varner – volume: 286 start-page: 38 issue: 1 year: 2000 end-page: 44 ident: CR38 article-title: Enzymatic synthesis and purification of caffeoyl-CoA, p-coumaroyl-CoA, and feruloyl-CoA publication-title: Anal Biochem doi: 10.1006/abio.2000.4760 contributor: fullname: Scheller – volume: 17 start-page: 125 issue: 2 year: 1998 end-page: 197 ident: CR4 article-title: Biosynthesis and genetic engineering of lignin publication-title: Crit Rev Plant Sci doi: 10.1016/S0735-2689(98)00360-8 contributor: fullname: Boerjan – volume: 48 start-page: 2221 issue: 20 year: 2003 end-page: 2225 ident: CR57 article-title: Cloning and expressional analyses of a cinnamoyl CoA reductase cDNA from rice seedlings publication-title: Chin Sci Bull doi: 10.1007/BF03182856 contributor: fullname: Yuxian – volume: 139 start-page: 259 issue: 2 year: 1984 end-page: 265 ident: CR43 article-title: Purification and properties of cinnamoyl-CoA reductase and cinnamyl alcohol dehydrogenase from poplar stems (Populus X euramericana) publication-title: Eur J Biochem doi: 10.1111/j.1432-1033.1984.tb08002.x contributor: fullname: Boudet – volume: 38 start-page: 671 issue: 4 year: 1998 end-page: 676 ident: CR41 article-title: Cloning and characterization of two maize cDNAs encoding cinnamoyl-CoA reductase (CCR) and differential expression of the corresponding genes publication-title: Plant Mol Biol doi: 10.1023/A:1006060101866 contributor: fullname: Grima-Pettenati – volume: 44 start-page: 605 issue: 4 year: 1997 end-page: 608 ident: CR36 article-title: Facile enzymic synthesis of caffeoyl CoA publication-title: Phytochemistry doi: 10.1016/S0031-9422(96)00594-8 contributor: fullname: Campbell – volume: 19 start-page: 3669 issue: 11 year: 2007 end-page: 3691 ident: CR27 article-title: Downregulation of cinnamoyl-coenzyme A reductase in poplar: multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure publication-title: Plant Cell doi: 10.1105/tpc.107.054148 contributor: fullname: Boerjan – volume: 275 start-page: 6537 issue: 9 year: 2000 end-page: 6545 ident: CR29 article-title: 5-hydroxyconiferyl aldehyde modulates enzymatic methylation for syringyl monolignol formation, a new view of monolignol biosynthesis in angiosperms publication-title: J Biol Chem doi: 10.1074/jbc.275.9.6537 contributor: fullname: Chiang – volume: 184 start-page: 237 issue: 1 year: 1977 end-page: 248 ident: CR21 article-title: 4-Coumarate:CoA ligase from cell suspension cultures of Petroselinum hortense Hoffm: partial purification, substrate specificity, and further properties publication-title: Arch Biochem Biophys doi: 10.1016/0003-9861(77)90347-2 contributor: fullname: Hahlbrock – volume: 157 start-page: 601 issue: 6 year: 2000 end-page: 607 ident: CR6 article-title: Induced phenylpropanoid metabolism during suberization and lignification: a comparative analysis publication-title: J Plant Physiol doi: 10.1016/S0176-1617(00)80002-4 contributor: fullname: Lewis – volume: 38 start-page: 513 issue: 4 year: 1998 end-page: 520 ident: CR37 article-title: Substrate profiles and expression of caffeoyl coenzyme A and caffeic acid O-methyltransferases in secondary xylem of aspen during seasonal development publication-title: Plant Mol Biol doi: 10.1023/A:1006071708728 contributor: fullname: Campbell – volume: 38 start-page: 305 issue: 4 year: 2003 end-page: 350 ident: CR5 article-title: Lignin: genetic engineering and impact on pulping publication-title: Crit Rev Biochem Mol Biol doi: 10.1080/10409230391036757 contributor: fullname: Boerjan – volume: 36 start-page: 6294 issue: 21 year: 1997 end-page: 6304 ident: CR47 article-title: Structural analysis of UDP-sugar binding to UDP-galactose 4-epimerase from Escherichia coli publication-title: Biochemistry doi: 10.1021/bi970025j contributor: fullname: Holden – volume: 159 start-page: 415 issue: 4 year: 2002 end-page: 422 ident: CR35 article-title: Isolation and characterization of a cinnamoyl-CoA reductase gene from perennial ryegrass (Lolium perenne) publication-title: J Plant Physiol doi: 10.1078/0176-1617-00719 contributor: fullname: Spangenberg – volume: 217 start-page: 218 issue: 2 year: 2003 end-page: 228 ident: CR13 article-title: Down-regulation of the AtCCR1 gene in Arabidopsis thaliana: effects on phenotype, lignins and cell wall degradability publication-title: Planta contributor: fullname: Jouanin – volume: 22 start-page: 3093 issue: 9 year: 2010 end-page: 3104 ident: CR18 article-title: Crystal structures of a Populus tomentosa 4-coumarate:CoA ligase shed light on its enzymatic mechanisms publication-title: Plant Cell doi: 10.1105/tpc.109.072652 contributor: fullname: Wang – volume: 52 start-page: 482 issue: 5 year: 2009 end-page: 491 ident: CR51 article-title: Identifying a cinnamoyl coenzyme A reductase (CCR) activity with 4-coumaric acid: coenzyme A ligase (4CL) reaction products in Populus tomentosa publication-title: J Plant Biol doi: 10.1007/s12374-009-9062-6 contributor: fullname: Xiang-Ning – volume: 268 start-page: 3354 issue: 11 year: 2001 end-page: 3359 ident: CR1 article-title: Benzalacetone synthase. A novel polyketide synthase that plays a crucial role in the biosynthesis of phenylbutanones in Rheum palmatum publication-title: Eur J Biochem doi: 10.1046/j.1432-1327.2001.02255.x contributor: fullname: Noguchi – ident: CR40 – volume: 23 start-page: 148 issue: 2 year: 2005 end-page: 148 ident: CR26 article-title: Biochemistry and molecular and biology of plants publication-title: Cell Biochem Funct doi: 10.1002/cbf.1131 contributor: fullname: Lazar – volume: 90 start-page: 4102 issue: 9 year: 1993 end-page: 4106 ident: CR46 article-title: Isolation and sequence of a cDNA encoding the Jerusalem artichoke cinnamate 4-hydroxylase, a major plant cytochrome P450 involved in the general phenylpropanoid pathway publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.90.9.4102 contributor: fullname: Werck-Reichhart – volume: 7 start-page: 1001 issue: 7 year: 1995 end-page: 1013 ident: CR53 article-title: Lignin biosynthesis publication-title: Plant Cell doi: 10.1105/tpc.7.7.1001 contributor: fullname: Sederoff – volume: 57 start-page: 1187 issue: 7 year: 2001 end-page: 1195 ident: CR25 article-title: Two cinnamoyl-CoA reductase (CCR) genes from Arabidopsis thaliana are differentially expressed during development and in response to infection with pathogenic bacteria publication-title: Phytochemistry doi: 10.1016/S0031-9422(01)00053-X contributor: fullname: Grima-Pettenati – volume: 117 start-page: 303 issue: 4 year: 2004 end-page: 310 ident: CR16 article-title: 4-Coumarate:coenzyme A ligase in black locust (Robinia pseudoacacia) catalyses the conversion of sinapate to sinapoyl-CoA publication-title: J Plant Res doi: 10.1007/s10265-004-0159-1 contributor: fullname: Tsutsumi – volume: 96 start-page: 10045 issue: 18 year: 1999 end-page: 10050 ident: CR19 article-title: New routes for lignin biosynthesis defined by biochemical characterization of recombinant ferulate 5-hydroxylase, a multifunctional cytochrome P450-dependent monooxygenase publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.96.18.10045 contributor: fullname: Chapple – volume: 40 start-page: 555 issue: 4 year: 1999 end-page: 565 ident: CR28 article-title: Secondary xylem-specific expression of caffeoyl-coenzyme A 3-O-methyltransferase plays an important role in the methylation pathway associated with lignin biosynthesis in loblolly pine publication-title: Plant Mol Biol doi: 10.1023/A:1006244325250 contributor: fullname: Chiang – volume: 22 start-page: 3357 issue: 10 year: 2010 end-page: 3373 ident: CR48 article-title: Functional analyses of caffeic acid O-methyltransferase and cinnamoyl-CoA-reductase genes from perennial ryegrass (Lolium perenne) publication-title: Plant Cell doi: 10.1105/tpc.109.072827 contributor: fullname: Spangenberg – volume: 58 start-page: 2011 issue: 8 year: 2007 end-page: 2021 ident: CR33 article-title: Characterization of a cinnamoyl-CoA reductase that is associated with stem development in wheat publication-title: J Exp Bot doi: 10.1093/jxb/erm064 contributor: fullname: Ma – volume: 43 start-page: 746 issue: 8 year: 2005 end-page: 753 ident: CR3 article-title: Kinetic and inhibition studies of cinnamoyl-CoA reductase 1 from Arabidopsis thaliana publication-title: Plant Physiol Biochem doi: 10.1016/j.plaphy.2005.06.003 contributor: fullname: Grima-Pettenati – volume: 157 start-page: 574 issue: 2 year: 2011 end-page: 586 ident: CR15 article-title: Functional characterization of evolutionarily divergent 4-coumarate:coenzyme a ligases in rice publication-title: Plant Physiol doi: 10.1104/pp.111.178301 contributor: fullname: Li – volume: 11 start-page: 429 issue: 3 year: 1997 end-page: 441 ident: CR23 article-title: Cinnamoyl CoA reductase, the first committed enzyme of the lignin branch biosynthetic pathway: cloning, expression and phylogenetic relationships publication-title: Plant J doi: 10.1046/j.1365-313X.1997.11030429.x contributor: fullname: Grima-Pettenati – volume: 16 start-page: 1446 issue: 6 year: 2004 end-page: 1465 ident: CR17 article-title: Silencing of hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesis publication-title: Plant Cell doi: 10.1105/tpc.020297 contributor: fullname: Legrand – volume: 59 start-page: 9 issue: 1 year: 1975 end-page: 15 ident: CR54 article-title: Purification and properties of isoenzymes of cinnamyl-alcohol dehydrogenase from soybean-cell-suspension cultures publication-title: Eur J Biochem doi: 10.1111/j.1432-1033.1975.tb02418.x contributor: fullname: Grisebach – volume: 119 start-page: 49 issue: 1 year: 1999 end-page: 56 ident: CR22 article-title: Regulation and functional expression of cinnamate 4-hydroxylase from parsley publication-title: Plant Physiol doi: 10.1104/pp.119.1.49 contributor: fullname: Hahlbrock – volume: 42 start-page: 475 issue: 5 year: 2001 end-page: 481 ident: CR2 article-title: Cloning and characterization of a hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase induced in response to UV-C and wounding from Capsicum annuum publication-title: Plant Cell Physiol doi: 10.1093/pcp/pce060 contributor: fullname: Kim – volume: 33 start-page: 1419 issue: 6 year: 1993 end-page: 1426 ident: CR9 article-title: Flavonoid biosynthesis in petals of Rhododendron simsii publication-title: Phytochemistry doi: 10.1016/0031-9422(93)85102-W contributor: fullname: Van Sumere – volume: 65 start-page: 529 issue: 2 year: 1976 end-page: 536 ident: CR52 article-title: Enzymic synthesis of lignin precursors. Purification and properties of a cinnamoyl-CoA: NADPH reductase from cell suspension cultures of soybean (Glycinemax) publication-title: Eur J Biochem doi: 10.1111/j.1432-1033.1976.tb10370.x contributor: fullname: Grisebach – volume: 42 start-page: 475 issue: 5 year: 2001 ident: 218_CR2 publication-title: Plant Cell Physiol doi: 10.1093/pcp/pce060 contributor: fullname: K Back – volume: 58 start-page: 2011 issue: 8 year: 2007 ident: 218_CR33 publication-title: J Exp Bot doi: 10.1093/jxb/erm064 contributor: fullname: QH Ma – volume: 6 start-page: 1427 issue: 10 year: 1994 ident: 218_CR56 publication-title: Plant Cell doi: 10.1105/tpc.6.10.1427 contributor: fullname: ZH Ye – volume: 7 start-page: 1001 issue: 7 year: 1995 ident: 218_CR53 publication-title: Plant Cell doi: 10.1105/tpc.7.7.1001 contributor: fullname: R Whetten – volume: 38 start-page: 305 issue: 4 year: 2003 ident: 218_CR5 publication-title: Crit Rev Biochem Mol Biol doi: 10.1080/10409230391036757 contributor: fullname: M Baucher – volume: 16 start-page: 1446 issue: 6 year: 2004 ident: 218_CR17 publication-title: Plant Cell doi: 10.1105/tpc.020297 contributor: fullname: L Hoffmann – volume: 386 start-page: 553 issue: 6 year: 2005 ident: 218_CR34 publication-title: Biol Chem contributor: fullname: QH Ma – volume: 11 start-page: 278 issue: 3 year: 2008 ident: 218_CR49 publication-title: Curr Opin Plant Biol doi: 10.1016/j.pbi.2008.03.005 contributor: fullname: R Vanholme – volume: 119 start-page: 115 issue: 1 year: 1981 ident: 218_CR32 publication-title: Eur J Biochem doi: 10.1111/j.1432-1033.1981.tb05584.x contributor: fullname: T Luderitz – volume: 275 start-page: 6537 issue: 9 year: 2000 ident: 218_CR29 publication-title: J Biol Chem doi: 10.1074/jbc.275.9.6537 contributor: fullname: L Li – volume: 159 start-page: 415 issue: 4 year: 2002 ident: 218_CR35 publication-title: J Plant Physiol doi: 10.1078/0176-1617-00719 contributor: fullname: R McInnes – volume: 117 start-page: 303 issue: 4 year: 2004 ident: 218_CR16 publication-title: J Plant Res doi: 10.1007/s10265-004-0159-1 contributor: fullname: K Hamada – volume: 38 start-page: 671 issue: 4 year: 1998 ident: 218_CR41 publication-title: Plant Mol Biol doi: 10.1023/A:1006060101866 contributor: fullname: M Pichon – volume: 157 start-page: 601 issue: 6 year: 2000 ident: 218_CR6 publication-title: J Plant Physiol doi: 10.1016/S0176-1617(00)80002-4 contributor: fullname: MA Bernards – volume: 90 start-page: 4102 issue: 9 year: 1993 ident: 218_CR46 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.90.9.4102 contributor: fullname: HG Teutsch – volume: 59 start-page: 9 issue: 1 year: 1975 ident: 218_CR54 publication-title: Eur J Biochem doi: 10.1111/j.1432-1033.1975.tb02418.x contributor: fullname: D Wyrambik – volume: 51 start-page: 139 issue: 2 year: 2002 ident: 218_CR10 publication-title: Acta Microbiol Pol contributor: fullname: AI El-Batal – volume: 13 start-page: 1567 issue: 7 year: 2001 ident: 218_CR30 publication-title: Plant Cell doi: 10.1105/tpc.13.7.1567 contributor: fullname: L Li – volume: 139 start-page: 259 issue: 2 year: 1984 ident: 218_CR43 publication-title: Eur J Biochem doi: 10.1111/j.1432-1033.1984.tb08002.x contributor: fullname: F Sarni – volume: 184 start-page: 237 issue: 1 year: 1977 ident: 218_CR21 publication-title: Arch Biochem Biophys doi: 10.1016/0003-9861(77)90347-2 contributor: fullname: K-H Knobloch – volume: 30 start-page: 352 issue: 3 year: 1975 ident: 218_CR45 publication-title: Z Naturforsch C Biosci doi: 10.1515/znc-1975-5-609 contributor: fullname: J Stockigt – volume: 57 start-page: 1187 issue: 7 year: 2001 ident: 218_CR25 publication-title: Phytochemistry doi: 10.1016/S0031-9422(01)00053-X contributor: fullname: V Lauvergeat – volume: 23 start-page: 148 issue: 2 year: 2005 ident: 218_CR26 publication-title: Cell Biochem Funct doi: 10.1002/cbf.1131 contributor: fullname: T Lazar – volume: 22 start-page: 3357 issue: 10 year: 2010 ident: 218_CR48 publication-title: Plant Cell doi: 10.1105/tpc.109.072827 contributor: fullname: Y Tu – volume: 185 start-page: 143 issue: 1 year: 2010 ident: 218_CR11 publication-title: New Phytol doi: 10.1111/j.1469-8137.2009.03018.x contributor: fullname: LL Escamilla-Trevino – volume: 48 start-page: 2221 issue: 20 year: 2003 ident: 218_CR57 publication-title: Chin Sci Bull doi: 10.1007/BF03182856 contributor: fullname: BAI Yong – volume: 217 start-page: 218 issue: 2 year: 2003 ident: 218_CR13 publication-title: Planta doi: 10.1007/s00425-003-0987-6 contributor: fullname: T Goujon – volume: 52 start-page: 482 issue: 5 year: 2009 ident: 218_CR51 publication-title: J Plant Biol doi: 10.1007/s12374-009-9062-6 contributor: fullname: D-D Wang – volume: 17 start-page: 125 issue: 2 year: 1998 ident: 218_CR4 publication-title: Crit Rev Plant Sci doi: 10.1016/S0735-2689(98)00360-8 contributor: fullname: M Baucher – volume: 17 start-page: 379 issue: 3 year: 2008 ident: 218_CR50 publication-title: Transgenic Res doi: 10.1007/s11248-007-9113-z contributor: fullname: J Wadenback – volume: 95 start-page: 12803 issue: 22 year: 1998 ident: 218_CR42 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.95.22.12803 contributor: fullname: J Ralph – volume: 143 start-page: 163 issue: 2 year: 1999 ident: 218_CR44 publication-title: Plant Sci doi: 10.1016/S0168-9452(99)00041-2 contributor: fullname: G Selman-Housein – volume: 36 start-page: 6294 issue: 21 year: 1997 ident: 218_CR47 publication-title: Biochemistry doi: 10.1021/bi970025j contributor: fullname: JB Thoden – volume: 22 start-page: 3093 issue: 9 year: 2010 ident: 218_CR18 publication-title: Plant Cell doi: 10.1105/tpc.109.072652 contributor: fullname: Y Hu – volume: 106 start-page: 625 issue: 2 year: 1994 ident: 218_CR12 publication-title: Plant Physiol doi: 10.1104/pp.106.2.625 contributor: fullname: D Goffner – volume: 286 start-page: 38 issue: 1 year: 2000 ident: 218_CR38 publication-title: Anal Biochem doi: 10.1006/abio.2000.4760 contributor: fullname: N Obel – volume: 96 start-page: 8955 issue: 16 year: 1999 ident: 218_CR39 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.96.16.8955 contributor: fullname: K Osakabe – volume: 160 start-page: 499 issue: 3 year: 2001 ident: 218_CR55 publication-title: Plant Sci doi: 10.1016/S0168-9452(00)00417-9 contributor: fullname: T Yabuya – volume: 38 start-page: 513 issue: 4 year: 1998 ident: 218_CR37 publication-title: Plant Mol Biol doi: 10.1023/A:1006071708728 contributor: fullname: H Meng – volume: 43 start-page: 746 issue: 8 year: 2005 ident: 218_CR3 publication-title: Plant Physiol Biochem doi: 10.1016/j.plaphy.2005.06.003 contributor: fullname: M Baltas – volume: 96 start-page: 10045 issue: 18 year: 1999 ident: 218_CR19 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.96.18.10045 contributor: fullname: JM Humphreys – volume: 119 start-page: 49 issue: 1 year: 1999 ident: 218_CR22 publication-title: Plant Physiol doi: 10.1104/pp.119.1.49 contributor: fullname: E Koopmann – volume: 40 start-page: 555 issue: 4 year: 1999 ident: 218_CR28 publication-title: Plant Mol Biol doi: 10.1023/A:1006244325250 contributor: fullname: L Li – volume: 268 start-page: 3354 issue: 11 year: 2001 ident: 218_CR1 publication-title: Eur J Biochem doi: 10.1046/j.1432-1327.2001.02255.x contributor: fullname: I Abe – volume: 302 start-page: 305 issue: 2 year: 2002 ident: 218_CR7 publication-title: Anal Biochem doi: 10.1006/abio.2001.5574 contributor: fullname: T Beuerle – volume: 11 start-page: 429 issue: 3 year: 1997 ident: 218_CR23 publication-title: Plant J doi: 10.1046/j.1365-313X.1997.11030429.x contributor: fullname: E Lacombe – volume: 157 start-page: 574 issue: 2 year: 2011 ident: 218_CR15 publication-title: Plant Physiol doi: 10.1104/pp.111.178301 contributor: fullname: J Gui – volume: 33 start-page: 1419 issue: 6 year: 1993 ident: 218_CR9 publication-title: Phytochemistry doi: 10.1016/0031-9422(93)85102-W contributor: fullname: L Cooman De – volume: 161 start-page: 105 issue: 1 year: 2004 ident: 218_CR24 publication-title: J Plant Physiol doi: 10.1078/0176-1617-01074 contributor: fullname: K Larsen – volume: 285 start-page: 277 issue: 1 year: 2010 ident: 218_CR8 publication-title: J Biol Chem doi: 10.1074/jbc.M109.036673 contributor: fullname: MW Bhuiya – volume: 65 start-page: 529 issue: 2 year: 1976 ident: 218_CR52 publication-title: Eur J Biochem doi: 10.1111/j.1432-1033.1976.tb10370.x contributor: fullname: H Wengenmayer – ident: 218_CR40 doi: 10.1007/s11033-012-2312-6 – volume: 103 start-page: 230 issue: 1 year: 2006 ident: 218_CR20 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0509875103 contributor: fullname: T Kawasaki – volume: 19 start-page: 3669 issue: 11 year: 2007 ident: 218_CR27 publication-title: Plant Cell doi: 10.1105/tpc.107.054148 contributor: fullname: JC Leple – volume: 267 start-page: 13903 issue: 20 year: 1992 ident: 218_CR14 publication-title: J Biol Chem doi: 10.1016/S0021-9258(19)49654-3 contributor: fullname: E Gout – volume: 44 start-page: 605 issue: 4 year: 1997 ident: 218_CR36 publication-title: Phytochemistry doi: 10.1016/S0031-9422(96)00594-8 contributor: fullname: H Meng – volume: 46 start-page: 1073 issue: 7 year: 2005 ident: 218_CR31 publication-title: Plant Cell Physiol doi: 10.1093/pcp/pci120 contributor: fullname: L Li |
SSID | ssj0045704 |
Score | 1.9977472 |
Snippet | Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44), which catalyzes the reduction of cinnamoyl-CoA esters to their respective cinnamaldehydes, is considered as a key... |
SourceID | proquest crossref springer fao |
SourceType | Aggregation Database Publisher |
StartPage | 348 |
SubjectTerms | acetic acid Biomedical and Life Sciences catalytic activity Cell Biology chromatography coumarate-CoA ligase coumaric acids enzyme activity Escherichia coli esters Life Sciences lignin methanol Original Article Plant Biochemistry polyacrylamide gel electrophoresis Populus tomentosa Protein Science Receptors |
Title | Preparation of cinnamoyl-CoA and analysis of the enzyme activity of recombinant CCR protein from Populus tomentosa |
URI | https://link.springer.com/article/10.1007/s13562-013-0218-6 https://search.proquest.com/docview/1660404380 |
Volume | 23 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwELba7YULDwHqQqmMxAnkVZyHNzmuVi0VCFQBkdqTNX6hql0HbbKH9td3JtlQlcehh-TgPJx4bM83Hs83jL0zrnKFAiuyUlVooIRcVD6fC5vYJFhrjSwoOPnLV3VS55_OirMdlv5euoiXs9Ej2U_Ud7FuGapq0ScjQLUk1C7bK4jva8L2Fh_PPx-N829ezPukgUSOJCiOcvRl_usl97TRboDmHtD8wzfaq5zjJ0MYYNszFdJOk8vZpjMze_M3j-MD_uYpe7xFoHwxdJlnbMfH52x9uvYDC3gTeRO4vYgRVs31lVg2Cw7R4TGwl9BVBI3cx5vrlecUF0HpJ6iYjOuV6bfW8OXyG-85IC4ipxAWfkqZwjYt74jyoWtaeMHq46MfyxOxTccgbFrNO1FKgFKhJe6yOeWTsCBTF0yCBSUUyhKNj8wdgE0DVElQocoMyJAaU0JwafaSTWIT_T7jJkgIibNlCFXubVYiKDNSZg7w8WCTKXs_ikX_Glg39B2_MjWdxqbT1HRaTdk-Ck7DT5wVdf09pTUcYjhFbDhlb0dpahw25AuB6JtNq6VSSd7T7U_Zh1FEejt-2_9X9upBd79mj_Bz8mHz3wGbdOuNf4MgpjOH2157iDD-vMZznS5uAQBc6xc |
link.rule.ids | 315,786,790,27955,27956,41114,41556,42183,42625,52144,52267 |
linkProvider | Springer Nature |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1La9tAEF6a9JBeSkMb4jaPDfTUsKDVYy0djWlwEjuExAbfltlXCcSrYskH59dnR7IwDumhB11WT2ZXM9_szHxDyE9lCpMJ0CzJRREcFJeywqZ9piMdOa214hkWJ0_uxGiW3syz-aaOu-qy3buQZKOpt8VuSbDVrOlGEOwSE3vkI9KpYx7fLB506jfN-k3PQORGYlhG2YUy33vEjjHac1Du4Mw3odHG4lx9IZ83UJEO2rk9JB-s_0qW90vb0nWXnpaO6ifvYVGun9mwHFDwJhwtzQieDeiOWv-yXliKBQzYJwKH0QteqCYHhg6HD7Qha3jyFGtN6D229FpVtEZuhrqs4BuZXf2eDkds0zeB6bjo1yznALkILrNJ-tj4QQOPjVNRGMghExr5dnhqAHTsoIiccEWigLtYqRyciZMjsu9Lb48JVY6Di4zOnStSq5M8oCfFeWIg3O501CO_OgHKvy09htwSIaO0ZZC2RGlL0SPHQcQS_gT1JWePMW62IBVpAHE9ctHJXYb1jUEL8LZcVZILEaUNL36PXHYTIjc_WvXvl33_r6vPycFoOhnL8fXd7Q_yKXxa2mbsnZD9ermypwF51OqsWWmv7OXQMg |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELboVkJceAhQlxYwEieQ2zhxvMlxtXQpFKoVsFI5mfGrqmCdapM9tL8eTx6qWsEBccjFeTke2zOTmfk-Ql5rW9pcgmFZIcvooHjBSicmzCQm8cYYzXMsTv58Io-W4uNpftrznNZDtvsQkuxqGhClKTQHF9YfXBe-ZVFvs5aZIOooJrfItoirVozI9vT99-PDYTMW-aRlEESkJIZFlUNg808PuaGatjxUN6zOW4HSVv_MH5AfQ8-7tJOf-5tG75urW6CO__FpD8n93jal024yPSJ3XHhM1ou16_DBq0ArT815CLCqLn-xWTWlEGw8OlwTPBvNSerC1eXKUayYQGIKbEa3e6XbpBs6m32hLTrEeaBY3EIXyCG2qWmDYBBNVcMTspwffpsdsZ6ogZm0nDSs4ACFjD66zSbINGGAp9brJDYUkEuDAD9cWACTeigTL32ZaeA-1boAb9PsKRmFKrgdQrXn4BNrCu9L4UxWRHNNc55ZiLd7k4zJm0FG6qLD41DXyMs4dCoOncKhU3JMdqIUFZzF_VItv6b4dwexT6PVOCavBtGquKAwSgLBVZtacSkT0QLxj8nbQVyqX9n131_27J-ufknuLt7N1acPJ8e75F7smegyBPfIqFlv3PNo6TT6RT-bfwMVmvXK |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Preparation+of+cinnamoyl-CoA+and+analysis+of+the+enzyme+activity+of+recombinant+CCR+protein+from+Populus+tomentosa&rft.jtitle=Journal+of+plant+biochemistry+and+biotechnology&rft.au=Han%2C+Botao&rft.au=Liu%2C+Shaoli&rft.au=Gai%2C+Ying&rft.au=Jiang%2C+Xiangning&rft.date=2014-10-01&rft.issn=0971-7811&rft.eissn=0974-1275&rft.volume=23&rft.issue=4&rft.spage=348&rft.epage=357&rft_id=info:doi/10.1007%2Fs13562-013-0218-6&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0971-7811&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0971-7811&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0971-7811&client=summon |